Mesenchymal stem versus stromal cells: International Society for Cell &amp; Gene Therapy (ISCT®) Mesenchymal Stromal Cell committee position statement on nomenclature

Viswanathan, Sowmya; Shi, Yufang; Galipeau, Jacques; Krampera, Mauro; LeBlanc, Katarina; Martin, Ivan; Nolta, Jan A.; Phinney, Donald G.; Sensebé, Luc

doi:10.1016/j.jcyt.2019.08.002

Cited by 494 publications

(384 citation statements)

References 60 publications

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“…1), suggesting that the cells must have undergone e Quantitative analysis of the relative protein expression (n = 3). *P < 0.05, **P < 0.01 vs. the control group; # P < 0.05, ## P < 0.01 vs. the H-EV group some changes in response to inflammation [48,49]. Indeed, the periodontium closely connects to the pulpdentin complex by three interconnected routes, namely, the apical foramen, the dentinal tubules, and the lateral canals, which means that once periodontitis involves a certain tooth, the dental pulp may be negatively affected by bacteria, especially by their byproducts residing in the periodontitis pocket [50,51].…”

Section: Discussionmentioning

confidence: 90%

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

Zhou

Yin

et al. 2020

Stem Cell Res Ther

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Background: Although dental pulp stem cells (DPSCs) isolated from periodontally compromised teeth (P-DPSCs) have been demonstrated to retain pluripotency and regenerative potential, their use as therapeutics remains largely unexplored. In this study, we investigated the proangiogenic effects of extracellular vesicles (EVs) secreted by P-DPSCs using in vitro and in vivo testing models. Methods: Patient-matched DPSCs derived from periodontally healthy teeth (H-DPSCs) were used as the control for P-DPSCs. Conditioned media (CMs) derived from H-DPSCs and P-DPSCs (H-CM and P-CM), CMs derived from both cell types pretreated with the EV secretion blocker GW4869 (H-GW and P-GW), and EVs secreted by H-DPSCs and P-DPSCs (H-EVs and P-EVs) were prepared to test their proangiogenic effects on endothelial cells (ECs). Cell proliferation, migration, and tube formation were assessed using the Cell Counting Kit-8 (CCK-8), transwell/scratch wound healing, and Matrigel assays, respectively. Specifically, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and western blot analysis were used to examine the expression levels of angiogenesis-related genes/proteins in ECs in response to EV-based incubation. Finally, a full-thickness skin defect model was applied to test the effects of EVs on wound healing and new vessel formation.

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Section: Discussionmentioning

confidence: 90%

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

Zhou

Yin

et al. 2020

Stem Cell Res Ther

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“…The stem cell character of hPDLCs was verified by investigating the expression of mesenchymal and hematopoietic stem cell surface markers (Table ), according to the International Society for Cell and Gene Therapy (Dominici et al, 2006; Viswanathan et al, 2019). Our single‐parameter analysis showed that more than 95% of hPDLCs were positively stained for CD29, CD73, CD90 and CD105.…”

Section: Resultsmentioning

confidence: 99%

“…Human periodontal ligament cells (hPDLCs) fulfil the minimal criteria of mesenchymal stem cells (MSCs) (Seo et al, 2004), including the expression of specific surface markers and a multilineage differentiation potential (Viswanathan et al., 2019). This heterogeneous population of fibroblast‐like cells participates in tissue regeneration by cell proliferation, differentiation into tissue‐specific cells and modulating immune and inflammatory responses (Racz et al, 2014; Wada, Gronthos, & Bartold, 2013; Xiao & Nasu, 2014).…”

Section: Introductionmentioning

confidence: 99%

Pleiotropic effects of vitamin D₃ on CD4⁺ T lymphocytes mediated by human periodontal ligament cells and inflammatory environment

Behm

Blufstein

Gahn

et al. 2020

J Clinic Periodontology

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Aims: Both, vitamin D 3 and human periodontal ligament cells (hPDLCs) possess immunosuppressive properties, but their combined effect on immune cells has never been investigated. Here, we analysed the impact of vitamin D 3 on the immunosuppressive properties of hPDLCs towards CD4 + T lymphocytes. Material and Methods:Allogenic CD4 + T lymphocytes were activated by phytohemagglutinin either in monoculture or co-culture with hPDLCs, in the presence or absence of IFN-γ and 1,25(OH) 2 D 3 . After 5 days, CD4 + T-lymphocyte proliferation, CD4 + CD25 + FoxP3 + regulatory T lymphocytes (T regs ) proportion and IL-10, TGF-β1 and IL-17A production were analysed. Results:In monoculture, 1,25(OH) 2 D 3 suppressed CD4 + T-lymphocyte proliferation, increased the percentage of CD4 + FoxP3 + CD25 + FoxP3 + T regs and enhanced IL-10 and TGF-β1 production. In the presence of IFN-γ treated hPDLCs, 1,25(OH) 2 D 3 significantly increased CD4 + T-lymphocyte proliferation and decreased the percentage of CD4 + CD25 + FoxP3 + T regs . IL-10 and IL-17A expression was significantly diminished by 1,25(OH) 2 D 3 , whereas TGF-β1 was slightly increased. The effects of 1,25(OH) 2 D 3 in co-culture were reversed by inhibition of indoleamine-2,3-dioxygenase-1, prostaglandin-endoperoxide synthase and programmed cell death 1 ligand 1. 1,25(OH) 2 D 3 also suppressed the expression of these proteins in hPDLCs. Conclusion:Effects of vitamin D 3 on CD4 + T lymphocyte are modified by hPDLCs depending on the microenvironment. K E Y W O R D SCD4-Positive T Lymphocytes, co-culture, Immunomodulation, Periodontal Ligament, Vitamin D

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“…Mesenchymal stromal cells (MSCs) are multipotent cells which are recognized for being a subset of non-hematopoietic adult stem cells originating from the mesoderm layer, with fibroblast-like morphology and multipotent potential [1,2]. MSCs are capable of differentiating into mesodermal lineages, such as adipocytes, osteocytes, or chondrocytes, and also into endodermic and neuroectodermic lineages, such as alveolar endothelial cells or neurons [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Role of Mesenchymal Stromal Cells as Therapeutic Agents: Potential Mechanisms of Action and Implications in Their Clinical Use

Jimenez-Puerta

Marchal

López‐Ruiz

et al. 2020

JCM

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Due to the great therapeutic interest that involves the translation of mesenchymal stromal cells (MSCs) into clinical practice, they have been widely studied as innovative drugs, in order to treat multiple pathologies. MSC-based cell therapy involves the administration of MSCs either locally or systemically into the receptor body where they can traffic and migrate towards the affected tissue and participate in the process of healing. The therapeutic effects of MSCs compromise of different mechanisms such as the functional integration of differentiated MSCs into diseased host tissue after transplantation, their paracrine support, and their impact on the regulation of both the innate and the acquired immune system. Here, we establish and provide recent advances about the principal mechanisms of action through which MSCs can perform their activity and effect as a therapeutic tool. The purpose of this review is to examine and discuss the MSCs capacity of migration, their paracrine effect, as well as MSC-mediated modifications on immune cell responses.

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Mesenchymal stem versus stromal cells: International Society for Cell & Gene Therapy (ISCT®) Mesenchymal Stromal Cell committee position statement on nomenclature

Cited by 494 publications

References 60 publications

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

Pleiotropic effects of vitamin D₃ on CD4⁺ T lymphocytes mediated by human periodontal ligament cells and inflammatory environment

Role of Mesenchymal Stromal Cells as Therapeutic Agents: Potential Mechanisms of Action and Implications in Their Clinical Use

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Mesenchymal stem versus stromal cells: International Society for Cell & Gene Therapy (ISCT®) Mesenchymal Stromal Cell committee position statement on nomenclature

Cited by 494 publications

References 60 publications

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth

Pleiotropic effects of vitamin D3 on CD4+ T lymphocytes mediated by human periodontal ligament cells and inflammatory environment

Role of Mesenchymal Stromal Cells as Therapeutic Agents: Potential Mechanisms of Action and Implications in Their Clinical Use

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Pleiotropic effects of vitamin D₃ on CD4⁺ T lymphocytes mediated by human periodontal ligament cells and inflammatory environment