Mesenchymal stem cells transmigrate across brain microvascular endothelial cell monolayers through transiently formed inter-endothelial gaps

Matsushita, T.; Kibayashi, Tatsuya; Katayama, Takahiro; Yamashita, Yuuki; Suzuki, Syuuichirou; Kawamata, Jun; Honmou, Osamu; Minami, Masabumi; Shimohama, Shun

doi:10.1016/j.neulet.2011.07.021

Cited by 86 publications

(62 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2D), including PLAU [21] and fibroblast growth factor (FGF) [33], which were involved in the formation of cell aggregation structures in a similar context. However, unexpectedly classical angiogenic stimulators ANGPT and vascular endothelial growth factor (VEGF) family [28,30,41,42] were not obviously induced in both cell types within this short period. The GO analysis in differential gene expression revealed significant correlation between angiogenesis and vascularization ( Fig.…”

Section: Rna-seq Revealed Differential Gene Expression Profiles and Amentioning

confidence: 90%

“…This result indicates that angiogenesis and prevascularization are involved in the cellcell communication and remodeling of the functional blood vessel development in vivo [5,[18][19][20][21][22][23][24][25][26]. Moreover, MSCs could repress ECs by regulating cytokine-induced leukocyte recruitment [27], while the activated ECs could regulate the MSCs-to-ECs transmigration in a leukocyte-like mechanism [28,29]. However, the innate mechanism of MSCsECs interaction-dependent vascularization remains elusive.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

LiJunxiang

MaYing

TengRuifang

et al. 2015

Stem Cells and Development

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) show great promise in blood vessel restoration and vascularization enhancement in many therapeutic situations. Typically, the co-implantation of MSCs with vascular endothelial cells (ECs) is effective for the induction of functional vascularization in vivo, indicating its potential applications in regenerative medicine. The effects of MSCs-ECs-induced vascularization can be modeled in vitro, providing simplified models for understanding their underlying communication. In this article, a contact coculture model in vitro and an RNA-seq approach were employed to reveal the active crosstalk between MSCs and ECs within a short time period at both morphological and transcriptional levels. The RNA-seq results suggested that angiogenic genes were significantly induced upon coculture, and this prevascularization commitment might require the NF-kB signaling. NF-kB blocking and interleukin (IL) neutralization experiments demonstrated that MSCs potentially secreted IL factors including IL1b and IL6 to modulate NF-kB signaling and downstream chemokines during coculture. Conversely, RNA-seq results indicated that the MSCs were regulated by the coculture environment to a smooth muscle commitment within this short period, which largely induced myocardin, the myogenic co-transcriptional factor. These findings demonstrate the mutual molecular mechanism of MSCs-ECs-induced prevascularization commitment in a quick response.

show abstract

Section: Rna-seq Revealed Differential Gene Expression Profiles and Amentioning

confidence: 90%

mentioning

confidence: 99%

Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

LiJunxiang

MaYing

TengRuifang

et al. 2015

Stem Cells and Development

View full text Add to dashboard Cite

show abstract

“…Whether systemically infused MSCs can actively migrate across BBB is still unclear. Rat MSCs were found to transmigrate across the BMEC monolayers in vitro through transient intercellular gaps between the BMECs [144] . However, the molecular mechanism underlying MSCs-BBB interaction is not known.…”

Section: Methods To Promote the Homing Of Mscs In The Treatment Of Cnmentioning

confidence: 99%

Strategies to improve the migration of mesenchymal stromal cells in cell therapy

Chen

et al. 2017

Transl. Neurosci. Clin.

View full text Add to dashboard Cite

KEYWORDSMSCs; migration; cell therapy; engraftment efficiency; neurological disorder Mesenchymal stromal/stem cells (MSCs) are multipotent cells under consideration as a potential new therapy for a variety of inflammatory diseases including certain neurological disorders. It is generally thought that the efficacy of cell therapy in attenuating damage after ischemia, inflammation, or injury depends on the quantity of transplanted cells recruited to the target tissue. However, only a small number of systematically infused MSCs can effectively migrate to target sites, which significantly decreases the efficacy of exogenous cell-based therapy. In this review, we discuss specific factors influencing MSC migration, and summarize current strategies that effectively promote the motility of MSCs. In addition, we describe several protocols to improve the migration of stromal cells into the nervous system and, therefore, enhance the efficiency of engraftment as means of treating neurological disorders.Citation Li G, Hu Y, Chen Y, Tang Z. Strategies to improve the migration of mesenchymal stromal cells in cell therapy. Transl. Neurosci. Clin. 2017, 3(3): 159-175.

show abstract

“…This results in alterations in the relative expression levels of pro and anti-inflammatory cytokines. Modulation of the immune system combined with the release of a range of trophic factors contributes to the capacity of MSCs to promote functional recovery in a number of settings and clinical trials are currently ongoing or planned using MSCs in the treatment of graft-vs-host disease, heart failure [7] and within the CNS, stroke, spinal cord injury and MS [11,23,24,39].…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Translating stem cell therapies to the clinic

Miller

Bai

2012

Neuroscience Letters

View full text Add to dashboard Cite

Mesenchymal stem cells transmigrate across brain microvascular endothelial cell monolayers through transiently formed inter-endothelial gaps

Cited by 86 publications

References 23 publications

Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

Strategies to improve the migration of mesenchymal stromal cells in cell therapy

Translating stem cell therapies to the clinic

Contact Info

Product

Resources

About