Mesenchymal stem cells rejuvenate cardiac muscle through regulating macrophage polarization

Zhang, Busheng; Zhao, Naishi; Zhang, Jing; Liu, Yu; Zhu, Dan; Kong, Ye

doi:10.18632/aging.102009

Cited by 17 publications

(19 citation statements)

References 33 publications

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“…44 Both arms of the inflammatory response are required for repair in many systems, such as heart, skeletal muscle, and the central nervous system. 32,41,45,46 Unregulated proinflammatory signals result in premature initiation of the anti-inflammatory cell signals, which can disrupt effective tissue healing. 41 For example, skeletal muscle regeneration is impaired when M F are prematurely activated by treatment with the anti-inflammatory cytokine IL-10.…”

Section: Inflammation and Tissue Regenerationmentioning

confidence: 99%

The Use of Pulsed Electromagnetic Field to Modulate Inflammation and Improve Tissue Regeneration: A Review

et al. 2019

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Pulsed electromagnetic field (PEMF) is emerging as innovative treatment for regulation of inflammation, which could have significant effects on tissue regeneration. PEMF modulates inflammatory processes through the regulation of pro-and anti-inflammatory cytokine secretion during different stages of inflammatory response. Consistent outcomes in studies involving animal and human tissue have shown promise for the use of PEMF as an alternative or complementary treatment to pharmaceutical therapies. Thus, PEMF treatment could provide a novel nonpharmaceutical means of modulating inflammation in injured tissues resulting in enhanced functional recovery. This review examines the effect of PEMF on immunomodulatory cells (e.g., mesenchymal stem/ stromal cells [MSCs] and macrophages [M F ]) to better understand the potential for PEMF therapy to modulate inflammatory signaling pathways and improve tissue regeneration. This review cites published data that support the use of PEMF to improve tissue regeneration. Our studies included herein confirm anti-inflammatory effects of PEMF on MSCs and M F .

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Section: Inflammation and Tissue Regenerationmentioning

confidence: 99%

The Use of Pulsed Electromagnetic Field to Modulate Inflammation and Improve Tissue Regeneration: A Review

et al. 2019

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“…Macrophages are hypothesized to play a critical role in inflammation by contributing to the exacerbation or reduction of inflammation. Macrophages can differentiate into M1- or M2-like macrophages, which are classically activated macrophages or alternatively activated macrophages [ 8 ] that have polarized phenotypes: pro-inflammatory (M1) or anti-inflammatory (M2). M1 macrophages initiate an inflammatory response and promote T helper cell type 1 (Th1) immunity, which is characterized by the release of proinflammatory cytokines such as tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-12, IL-6, and arginase (ARG)-1 [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cells Decrease M1/M2 Ratio and Alleviate Inflammation to Improve Limb Ischemia in Mice

Song

Zhang²,

et al. 2020

Med Sci Monit

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Background Limb ischemia (LI) is the underlying pathology of peripheral artery disease (PAD). Macrophages play a critical role in inflammation and can contribute to the exacerbation or reduction of inflammation. Transplantation of mesenchymal stem cells (MSCs) is an emerging therapeutic strategy for PAD. However, the mechanism by which human placenta-derived mesenchymal stem cells (PMSCs) regulate macrophage differentiation in ischemic tissue remains unclear. Material/Methods Placentas were obtained from healthy donors with normal 38- to 40-week gestation, and PMSCs were isolated from the placentas and cultured. A mouse model of hind-limb ischemia was established. Ischemic limbs were injected intramuscularly with about 5×10 6 PMSCs in the PMSCs group or a placebo solution (phosphate-buffered saline) in the control group at 4 different sites 1 day after the procedure. The blood perfusion of hind-limbs and the histological morphology were observed at day 1, 7, and 14 after the surgical procedure. Macrophages were detected by flow cytometry. The expression of serum tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA). The expression of CD31 and smooth muscle α-actin (α-SMA) in frozen muscle samples were detected by immunofluorescence staining. Results In the PMSCs group, blood perfusion was gradually recovered and ischemic injury was markedly alleviated. The percentage of M2-like macrophages was increased dramatically, while the M1/M2 macrophage ratio was reduced. The expression of TNF-α and IL-6 was reduced, while the IL-10 level was elevated. The expression and density of CD31- and α-SMA-positive vessels were both significantly increased. Conclusions Transplanted PMSCs alleviated inflammation, promoted neovascularization, and improved hind limb ischemia through regulating macrophage differentiation toward the M2 phenotype and cytokine secretion. Cytokine manipulation of macrophage phenotypes may have potential therapeutic benefits in injured ischemic limbs.

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“…While BMSCs show their therapeutic effects on cardiac remodeling process through paracrine effects, studies have also indicated that immunomodulatory effects can also show their protective effects [9][10][11][12][13][14], such as modulating the function of regulatory T cells (Tregs) and reducing the proliferation of T cells. T cells, a central player in the adaptive immune system, take an important role in affecting acute MI and cardiac remodeling via their proliferation and differentiation [15].…”

Section: Immuno-and Inflammatory Modulation By Bmscs Therapy In Revermentioning

confidence: 99%

“…), with decreased interferongamma (IFN-γ) production, to an anti-inflammatory state with an increase in interleukin 10 (IL-10) production by Tregs [10]. Moreover, BMSCs release antiinflammatory cytokines to affect the macrophage M2 polarization in MI [11]. Of note, inflammation resolution is an important phase for reducing scar formation and protecting cardiac function after MI [16].…”

Section: Immuno-and Inflammatory Modulation By Bmscs Therapy In Revermentioning

confidence: 99%

The involving progress of MSCs based therapy in atherosclerosis

2020

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Atherosclerosis is a chronic progressive vascular inflammation characterized by lipid deposition and plaque formation, for which vascular cell dysfunction and impaired immune responses are involved. Up to now, lipidlowering drugs remain the main therapy for treating atherosclerosis; however, the surgical or interventional therapy is often applied, and yet, morbidity and mortality of such cardiovascular disease remain high worldwide. Over the past decades, an anti-inflammatory approach has become an important therapeutic target for dealing with atherosclerosis, as altered immune responses have been regarded as an essential player in the pathological process of vascular abnormality induced by hyperlipidemia. Interestingly, mesenchymal stem cells, one type of stem cells with the capabilities of self-renewal and multi-potential, have demonstrated their unique immunomodulatory function in the various pathological process, especially in atherosclerosis. While some controversies remain regarding their therapeutic efficacy and working mechanisms, our present review aims to summarize the current research progress on stem cell-based therapy, focusing on its immunomodulatory effects on the pathogenesis of atherosclerosis and how endothelial cells, smooth muscle cells, and other immune cells are regulated by MSCbased therapy.

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Mesenchymal stem cells rejuvenate cardiac muscle through regulating macrophage polarization

Cited by 17 publications

References 33 publications

The Use of Pulsed Electromagnetic Field to Modulate Inflammation and Improve Tissue Regeneration: A Review

The Use of Pulsed Electromagnetic Field to Modulate Inflammation and Improve Tissue Regeneration: A Review

Mesenchymal Stem Cells Decrease M1/M2 Ratio and Alleviate Inflammation to Improve Limb Ischemia in Mice

The involving progress of MSCs based therapy in atherosclerosis

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