Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton’s jelly

Trivanović, Drenka; Kocić, Jelena; Mojsilović, Slavko; Krstić, Aleksandra; Ilić, Vesna; Djordjević, Ivana; Santibáñez, Juan F.; Jovčić, Gordana; Terzić, Milan; Bugarski, Diana

doi:10.2298/sarh1304178t

Cited by 64 publications

(51 citation statements)

References 30 publications

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“…Conversely, the use of MSCs is free of such ethical concerns and requires less stringent criteria for transplantation [1]. MSCs are stem cells of stromal origin characterized by their multipotency with the ability to self-renew [2] [3] and differentiate to diverse cell phenotypes such as osteoblasts, chondrocytes, adipocytes, fibroblasts, tenocytes [4] [5], hepatocytes, neural cells and/or epithelial cells [6]. Due to their crucial regenerative, reparative, angiogenic and immunomodulatory properties, MSCs have generated increased interest in a variety of biomedical disciplines and several areas of experimental and clinical applications [2].…”

Section: Introductionmentioning

confidence: 99%

“…MSCs are stem cells of stromal origin characterized by their multipotency with the ability to self-renew [2] [3] and differentiate to diverse cell phenotypes such as osteoblasts, chondrocytes, adipocytes, fibroblasts, tenocytes [4] [5], hepatocytes, neural cells and/or epithelial cells [6]. Due to their crucial regenerative, reparative, angiogenic and immunomodulatory properties, MSCs have generated increased interest in a variety of biomedical disciplines and several areas of experimental and clinical applications [2]. Surely, marrow MSCs (BM MSCs) have been the most widely studied; however MSCs can be also isolated, with similar but not identical features, from different tissues including umbilical blood cord (UCB MSCs), placenta, adipose tissue (AT MSCs), trabecular bone, dental pulp and peripheral blood (PB MSCs) [7]- [12].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of Multipotent and Pluripotent Stem Cells from Human Peripheral Blood

Gargiulo¹,

Pham²,

Hai³

et al. 2015

SCD

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Stem cells are commonly classified based on the developmental stage from which they are isolated, although this has been a source of debate amongst stem cell scientists. A common approach classifies stem cells into three different groupings: Embryonic Stem Cells (ESCs), Umbilical Cord Stem Cells (UCBSCs) and Adult Stem Cells (ASCs), which include stem cells from bone marrow (BM), fat tissue (FT), engineered induced pluripotent (IP) and peripheral blood (PB). By definition stem cells are progenitor cells capable of self-renewal and differentiation hypothetically "ab infinitum" into more specialized cells and tissue. The main intent of this study was to determine and characterize the different sub-groups of stem cells present within the human PB-SCs that may represent a valid opportunity in the field of clinical regenerative medicine. Stem cells in the isolated mononucleated cells were characterized using a multidisciplinary approach that was based on morphology, the expression of stem cell markers by flowcytometry and fluorescence analysis, RT-PCR and the capacity to self-renew or proliferate and differentiate into specialized cells. This approach was used to identify the expression of hematopoietic, mesenchymal, embryonic and neural stem cell markers. Both isolated adherent and suspension mononucleated cells were able to maintain their stem cell properties during in-vitro culture by holding their capacity for proliferation and differentiation into osteoblast cells, respectively, when exposed to the appropriate induction medium.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Multipotent and Pluripotent Stem Cells from Human Peripheral Blood

Gargiulo¹,

Pham²,

Hai³

et al. 2015

SCD

View full text Add to dashboard Cite

show abstract

“…In our laboratory MSCs from adipose tissue, dental pulp of deciduous teeth, peripheral blood and Wharton's jelly of umbilical cord were recently isolated and characterized exhibiting typical MSC morphology, clonogenic potential, immunophenotype and multipotent capacity to differentiate along mesenchymal lineages (osteogenic, chondrogenic, adipogenic and myogenic) in vitro [10,11]. It is known that MSCs isolated from different tissues have similar but not identical capacity to modulate immune response.…”

Section: Discussionmentioning

confidence: 99%

“…MCSs from human dental pulp of deciduous teeth (DP-MSCs), peripheral blood (PB-MSCs), umbilical cord Wharton's jelly (UC-MSCs), as well as from adipose tissue (AT-MSCs), were isolated and their MSCs identity confirmed as previously described [10,11].…”

Section: Mscs Isolation and Culturementioning

confidence: 99%

Immunomodulatory capacity of human mesenchymal stem cells isolated from adipose tissue, dental pulp, peripheral blood and umbilical cord Wharton’s jelly

Trivanović

Mojsilović²,

Ilić³

et al. 2013

cejoi

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“…These cells continue to have their normal karyotype after multiple cycles of cell division (Mitchell et al 2003;Can and Karahuseyinoglu 2007). These cells could also differentiate in to other cells including osteoblasts, adipocyte, myocyte, and endothelial progenitor cells (Shen et al 2013;Trivanović et al 2013). Researches showed that MSCs obtained from this region have the potential to express neuronal markers and could be a suitable source for treating the symptoms of Parkinson disease (Fu et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Induction of human umbilical Wharton’s jelly-derived mesenchymal stem cells toward motor neuron-like cells

Bagher

Ebrahimi‐Barough

Azami

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

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The most important property of stem cells from different sources is the capacity to differentiate into various cells and tissue types. However, problems including contamination, normal karyotype, and ethical issues cause many limitations in obtaining and using these cells from different sources. The cells in Wharton's jelly region of umbilical cord represent a pool source of primitive cells with properties of mesenchymal stem cells (MSCs). The aim of this study was to determine the potential of human Wharton's jelly-derived mesenchymal stem cells (WJMSCs) for differentiation to motor neuron cells. WJMSCs were induced to differentiate into motor neuron-like cells by using different signaling molecules and neurotrophic factors in vitro. Differentiated neurons were then characterized for expression of motor neuron markers including nestin, PAX6, NF-H, Islet 1, HB9, and choline acetyl transferase (ChAT) by quantitative reverse transcription PCR and immunocytochemistry. Our results showed that differentiated WJMSCs could significantly express motor neuron biomarkers in RNA and protein levels 15 d post induction. These results suggested that WJMSCs can differentiate to motor neuron-like cells and might provide a potential source in cell therapy for neurodegenerative disease.

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Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton’s jelly

Abstract: The results obtained demonstrated that both MSCs represent good alternative sources of adult MSCs that could be used in cell therapy applications.

Cited by 64 publications

References 30 publications

Isolation and Characterization of Multipotent and Pluripotent Stem Cells from Human Peripheral Blood

Isolation and Characterization of Multipotent and Pluripotent Stem Cells from Human Peripheral Blood

Immunomodulatory capacity of human mesenchymal stem cells isolated from adipose tissue, dental pulp, peripheral blood and umbilical cord Wharton’s jelly

Induction of human umbilical Wharton’s jelly-derived mesenchymal stem cells toward motor neuron-like cells

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