Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine Wound Healing Model

Abstract

Wound healing of acute full-thickness injuries and chronic non-healing ulcers leads to delayed wound closure, prolonged recovery period and hypertrophic scarring, generating a demand for an autologous cell therapy and a relevant pre-clinical research models for wound healing. In this study, an immunocompetent model for wound healing was employed using a syngeneic murine cell line of mesenchymal stem cells cultured from the mouse whisker hair follicle outer root sheath (named moMSCORS). moMSCORS were isolated using an air-liquid interface method, expanded in vitro and characterized according to the MSC definition criteria - cell viability, in vitro proliferation, MSC phenotype and multi-lineage differentiations. Moreover, upon applying moMSCORS in an in vivo full-thickness wound model in the syngeneic C57BL/6 mice, the treated wounds displayed different morphology to that of the untreated wound beds. Quantitative evaluation of angiogenesis, granulation and wound closure involving clinical scoring and software-based quantification indicated a lower degree of inflammation in the treated wounds. Histological staining of treated wounds by the means of H&E, Alcian Blue, PicroSirius Red and αSMA immune labelling showed lower cellularity, less collagen filaments as well as thinner dermal and epidermal layers compared with the untreated wounds, indicating a general reduction of hypertrophic scars. The decreased inflammation, accelerated wound closure and non-hypertrophic scarring, which were facilitated by moMSCORS, hereby address a common problem of hypertrophic scars and non-physiological tissue properties upon wound closure, and additionally offer an in vivo model for the autologous cell-based wound healing. Graphic Abstract

Double Cross‐Linked Biomimetic Hyaluronic Acid‐Based Hydrogels with Thermo‐Stimulated Self‐Contraction and Tissue Adhesiveness for Accelerating Post‐Wound Closure and Wound Healing

“…Compared to PNI‐1.0HA, a larger area of dark‐blue staining (indicating more collagen deposition) was found in the control and PNI groups, signifying the higher rate of scar tissue formation. [ 69 ] This suggests that the introduction of HA contributes to normalized skin regeneration rather than excessive fibroblast proliferation for scar formation.…”

Flexible, biodegradable ultrasonic wireless electrotherapy device based on highly self-aligned piezoelectric biofilms

“…For H&E and Masson’s trichrome staining, mice implanted with biofilms were euthanized on days 6 and 12, and all tissue samples were fixed with 4% paraformaldehyde and embedded in paraffin. H&E and Masson’s trichrome staining was then carried out in accordance with the established protocols ( 51 ), and the representative slices were photographed with an optical microscope (Olympus digital camera, Olympus, Japan).…”

Novel recombinant R-spondin1 promotes hair regeneration by targeting the Wnt/β-catenin signaling pathway

“…mHFSCs were isolated and cultured following a previously published protocol [ 18 , 19 ] . Briefly, hair follicles were microdissected from a skin sample under an MZ62 dissecting microscope (Mshot, Guangzhou, China).…”