Mesenchymal Stem Cells from Human Dental Pulp: Isolation, Characteristics, and Potencies of Targeted Differentiation

Полтавцева, Р. А.; Nikonova, Yu. A.; Селезнева, И. И.; Yaroslavtseva, A. K.; Степаненко, В. Н.; Есипов, Р. С.; Павлович, С. В.; Klimantsev, I. V.; Tyutyunnik, N. V.; Grebennik, T. K.; Nikolaeva, A. V.; Сухих, Г. Т.

doi:10.1007/s10517-014-2714-7

Cited by 32 publications

(20 citation statements)

References 10 publications

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“…Stirred tank bioreactor culture combined with serum-/ xenogeneic-free culture medium enables an efficient expansion of umbilical cord-derived mesenchymal stem/stromal cells MSC are multipotent stem cells and constitute a heterogeneous subset of stromal stem cells that can be isolated from a variety of tissues such as bone marrow (BM) [2], umbilical cord matrix (UCM) [3], adipose tissue (AT) [4], placenta [5] and dental pulp [6]. Although BM is the most commonly used and well-studied MSC source, in the last decade the therapeutic potential of MSC isolated from neonatal tissues, namely UCM have also been demonstrated [7,8].…”

Section: Research Articlementioning

confidence: 99%

Stirred tank bioreactor culture combined with serum‐/xenogeneic‐free culture medium enables an efficient expansion of umbilical cord‐derived mesenchymal stem/stromal cells

Mizukami

Fernandes‐Platzgummer

Carmelo

et al. 2016

Biotechnology Journal

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Mesenchymal stem/stromal cells (MSC) are being widely explored as promising candidates for cell-based therapies. Among the different human MSC origins exploited, umbilical cord represents an attractive and readily available source of MSC that involves a non-invasive collection procedure. In order to achieve relevant cell numbers of human MSC for clinical applications, it is crucial to develop scalable culture systems that allow bioprocess control and monitoring, combined with the use of serum/xenogeneic (xeno)-free culture media. In the present study, we firstly established a spinner flask culture system combining gelatin-based Cultispher(®) S microcarriers and xeno-free culture medium for the expansion of umbilical cord matrix (UCM)-derived MSC. This system enabled the production of 2.4 (±1.1) x10(5) cells/mL (n = 4) after 5 days of culture, corresponding to a 5.3 (±1.6)-fold increase in cell number. The established protocol was then implemented in a stirred-tank bioreactor (800 mL working volume) (n = 3) yielding 115 million cells after 4 days. Upon expansion under stirred conditions, cells retained their differentiation ability and immunomodulatory potential. The development of a scalable microcarrier-based stirred culture system, using xeno-free culture medium that suits the intrinsic features of UCM-derived MSC represents an important step towards a GMP compliant large-scale production platform for these promising cell therapy candidates.

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Section: Research Articlementioning

confidence: 99%

Stirred tank bioreactor culture combined with serum‐/xenogeneic‐free culture medium enables an efficient expansion of umbilical cord‐derived mesenchymal stem/stromal cells

Mizukami

Fernandes‐Platzgummer

Carmelo

et al. 2016

Biotechnology Journal

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“…Dental pulp cells (DPCs) with self-renewal, colony forming efficiency, and multilineage differentiation capability are promising cell source for dental tissue regeneration [1, 2]. It is verified that DPCs were capable of differentiating into odontoblasts, adipocytes, chondrocytes, and so forth [1].…”

Section: Introductionmentioning

confidence: 99%

“…It is verified that DPCs were capable of differentiating into odontoblasts, adipocytes, chondrocytes, and so forth [1]. Since DPCs are easily obtained from extracted teeth, they may be ideal cell resource to repair injured tooth structures.…”

Section: Introductionmentioning

confidence: 99%

Effect of Luteolin and Apigenin on the Expression of Oct-4, Sox2, and c-Myc in Dental Pulp Cells withIn VitroCulture

Liu

Peng

et al. 2015

BioMed Research International

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Introduction. Dental pulp cells (DPCs) are promising cell source for dental tissue regeneration. Recently, small molecules which optimize microenvironment or activate the reprogramming network provide a new way to enhance the pluripotency. Two promising bioflavonoids luteolin and apigenin were reported to enhance reprogramming efficiency in mouse embryonic fibroblast (MEF). However, their effect and underlying mechanism in cell fate determination of human DPCs remain unclear. Methods. To elucidate the effect of luteolin and apigenin on the cell fate determination of DPCs, we explored the cell proliferation, cell cycle, senescence, apoptosis, expression of pluripotency markers Oct-4, Sox2, and c-Myc, and multilineage differentiation capability of DPCs with luteolin or apigenin treatment. Results. We demonstrated that luteolin and apigenin inhibited cell proliferation, arrested DPCs in G2/M and S phase, and upregulated PI value and apoptosis. Moreover, luteolin and apigenin increased telomerase activity, maintained DPCs in a presenescent state, and activated the expression of Oct-4, Sox2, and c-Myc at a dose- and time-dependent pattern in DPCs even at late passages, albeit repressed lineage-specific differentiation. Conclusions. Addition of luteolin and apigenin in the culture medium might provide an effective way to maintain DPCs in an undifferentiated stage and inhibit lineage-specific differentiation.

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“…Human tooth postnatal DPSCs were isolated from the rudiment of the third molar extracted for orthodontic indications, as described earlier in [ 23 ]. The cells were grown in the DMEM medium with the addition of 10% Fetal Bovine Serum (FBS, HyClone) in a humidified incubator (Binder, Tuttlingen, Germany) at 37 °C and 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

In Vitro Properties of Manganese-Substituted Tricalcium Phosphate Coatings for Titanium Biomedical Implants Deposited by Arc Plasma

et al. 2020

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Bioactive manganese (Mn)-doped ceramic coatings for intraosseous titanium (Ti) implants are developed. Arc plasma deposition procedure is used for coatings preparation. X-ray Diffraction, Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy, and Electron Paramagnetic Resonance (EPR) methods are applied for coatings characterization. The coatings are homogeneous, composed of the main phase α-tricalcium phosphate (α-TCP) (about 67%) and the minor phase hydroxyapatite (about 33%), and the Mn content is 2.3 wt%. EPR spectroscopy demonstrates that the Mn ions are incorporated in the TCP structure and are present in the coating in Mn2+ and Mn3+ oxidation states, being aggregated in clusters. The wetting contact angle of the deposited coatings is suitable for cells’ adhesion and proliferation. In vitro soaking in physiological solution for 90 days leads to a drastic change in phase composition; the transformation into calcium carbonate and octacalcium phosphate takes place, and no more Mn is present. The absence of antibacterial activity against Escherichia coli, Enterococcus faecalis, and Pseudomonas aeruginosa bacteria strains is observed. A study of the metabolic activity of mouse fibroblasts of the NCTC L929 cell line on the coatings using the MTT (dye compound 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test demonstrates that there is no toxic effect on the cell culture. Moreover, the coating material supports the adhesion and proliferation of the cells. A good adhesion, spreading, and proliferative activity of the human tooth postnatal dental pulp stem cells (DPSC) is demonstrated. The developed coatings are promising for implant application in orthopedics and dentistry.

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Mesenchymal Stem Cells from Human Dental Pulp: Isolation, Characteristics, and Potencies of Targeted Differentiation

Cited by 32 publications

References 10 publications

Stirred tank bioreactor culture combined with serum‐/xenogeneic‐free culture medium enables an efficient expansion of umbilical cord‐derived mesenchymal stem/stromal cells

Stirred tank bioreactor culture combined with serum‐/xenogeneic‐free culture medium enables an efficient expansion of umbilical cord‐derived mesenchymal stem/stromal cells

Effect of Luteolin and Apigenin on the Expression of Oct-4, Sox2, and c-Myc in Dental Pulp Cells withIn VitroCulture

In Vitro Properties of Manganese-Substituted Tricalcium Phosphate Coatings for Titanium Biomedical Implants Deposited by Arc Plasma

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