Mesenchymal Stem Cells Enhance Immune Response and Protectmice Against Lethal Herpes Viral Infection

Климова, Р. Р.; Momotyuk, Ekaterina; Demidova, N. A.; Yarigina, E. I.; Кущ, А А

doi:10.18821/0507-4088-2018-63-6-261-267

Cited by 5 publications

(5 citation statements)

References 22 publications

(22 reference statements)

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“…The immunological activity of these cytokines can be judged by their antiviral action. Previously, it was shown that the culture fluids from the MSCs on the fourth passage, containing the maximum concentrations of IL-6 and TNF-α, showed the maximum effect of suppressing the infectious activity of the herpes simplex virus (HSV-1) in vitro [ 22 ]. Therefore, to study the immunostimulatory activity of MSCs in vivo, we injected mice with MSCs collected at 3–4 passages of cultivation, when the functional activity and proinflammatory properties of the MSCs were high.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cells Can Both Enhance and Inhibit the Cellular Response to DNA Immunization by Genes of Nonstructural Proteins of the Hepatitis C Virus

Масалова

Леснова

Климова

et al. 2021

IJMS

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Despite extensive research, there is still no vaccine against the hepatitis C virus (HCV). The aim of this study was to investigate whether MSCs can exhibit adjuvant properties during DNA vaccination against hepatitis C. We used the pcNS3-NS5B plasmid encoding five nonstructural HCV proteins and MSCs derived from mice bone marrow. Five groups of DBA mice were immunized with the plasmid and/or MSCs in a different order. Group 1 was injected with the plasmid twice at intervals of 3 weeks; Group 2 with the plasmid, and after 24 h with MSCs; Group 3 with MSCs followed by the plasmid the next day; Group 4 with only MSCs; and Group 5 with saline. When the MSCs were injected prior to DNA immunization, the cell immune response to HCV proteins assessed by the level of IFN-γ synthesis was markedly increased compared to DNA alone. In contrast, MSCs injected after DNA suppressed the immune response. Apparently, the high level of proinflammatory cytokines detected after DNA injection promotes the conversion of MSCs introduced later into the immunosuppressive MSC2. The low level of cytokines in mice before MSC administration promotes the high immunostimulatory activity of MSC1 in response to a DNA vaccine. Thus, when administered before DNA, MSCs are capable of exhibiting promising adjuvant properties.

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Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cells Can Both Enhance and Inhibit the Cellular Response to DNA Immunization by Genes of Nonstructural Proteins of the Hepatitis C Virus

Масалова

Леснова

Климова

et al. 2021

IJMS

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“…Cell cultures . The primary culture of MSCs was obtained from the red bone marrow of mice, as described previously [ 23 ]. We have shown [ 22 , 23 ] that in terms of adhesive ability, surface markers, and differentiation potential, these cells meet the criteria for determination as MSCs by the International Society for Cellular Therapy [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

“…Detection of antibodies to HSV-1 . Titers of HSV-1-specific antibodies were determined in mouse sera by ELISA, as described previously [ 23 ]. To detect HSV specific immunoglobulins of the IgG1 and IgG2a subclasses, 96-well plates were sensitized with iHSV at a volume of 100 μL/well at a concentration of 50 μg/mL overnight at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Preventive Vaccination with Mesenchymal Stem Cells Protects Mice from Lethal Infection Caused by Herpes Simplex Virus 1

et al. 2021

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Herpes simplex viruses 1 and 2 (HSV-1 and HSV-2) infect almost all organs and tissues, cause genital herpes—the most common sexually transmitted disease—disorders of the central nervous system (CNS), and lead to severe complications in children. Despite the available drugs, the incidence of HSV-1/2 continues to rise. None of the prophylactic vaccine candidates have shown a protective effect in trials nor approval for use in clinical practice. We have investigated the protective properties of mesenchymal stem cells (MSC) isolated from the bone marrow of mice. A comparative analysis of the protective response to the introduction of primary and modified MSCs (mMSC) was carried out using the plasmid containing gene of the HSV and an inactivated virus in a model of lethal HSV-1 infection in mice. mMSCs were obtained by transfection of the Us6 gene encoding glycoprotein D (gD) of the HSV, the plasmid contained the same gene. After twofold immunization with primary MSCs, the formation of antibodies interacting with the viral antigen (according to enzyme immunoassay data) and neutralizing the infectious activity of HSV-1 in the reaction of biological neutralization was observed in the peripheral blood of mice. In addition, the introduction of primary MSCs induced the production of interferon gamma (INF-γ) which is detected in the peripheral blood of mice. After infection with HSV-1, the immunized mice showed significantly increased titers of virus-specific antibodies, an increased level of IFNγ, and were completely protected from lethal HSV-1 infection. The protective effect of the other three immunogens was lower and did not exceed 50–65%. Considering the wide availability of MSCs, the proven safety of intravenous administration, and the results obtained in this work on the ability to induce innate, adaptive and protective immunity to HSV-1, MSCs can be considered a promising basis for the development of new cellular vaccines for the prevention of herpesvirus and other viral infections.

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“…Also, it is worth to mention that exosome derived from MSCs (EX-MSCs) has tested for its protection as well as therapeutic potential in some viral diseases as Hepatitis C Virus (HCV), Herpes Virus (HSV), and influenza virus at in vitro and in vivo [34][35][36]. Moreover, exosomes can play a significant role in rejuvenating degenerated lung tissues, secondary to SARS-CoV-2, as it possesses antioxidant, antifibrotic, anti-inflammatory, anti-apoptotic, matrix integrity, and other regeneration cues [37].…”

Section: Methods/source Of Datamentioning

confidence: 99%

Cell Therapy as an Alternative approach for COVID-19 Infection Consequences: A Non-Systematic Review

Elkhenany

Gupta²,

Abdelhai

2021

IJCV

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The current uncontrollable outbreak of novel coronavirus (COVID-19) has unleashed severe global consequences in all aspects of life and society, bringing the whole world to a complete halt and has modeled significant threats to the global economy. The COVID-19 infection manifests with flu-like symptoms such as cough, cold, and fever resulting in acute respiratory distress syndrome (ARDS), lung dysfunction, and other systemic complications in critical patients are creating panic across the globe. However, the licensed vaccine has started to show up; some resulted in side effects that would limit its possibility in some circumstances as allergic personnel, for example. Moreover, the production and approval of new drugs is a very complicated process and takes a long time. On the other hand, stem cells have gone the extra mile and intensively investigated at preclinical and clinical studies in various degenerative diseases, including infectious ones. Stem cells are proposed as a broad-spectrum therapeutic agent, which may suppress the exaggerated immune response and promote endogenous repair by enhancing COVID-19 infected lung microenvironment. Also, stem cells have different application manners, either direct transplantation, exosome transplantation, or drug delivery of specific cytokines or nanoparticles with antiviral property by engineering stem cells. This review discusses and summarizes the possible emerging role of cell-based therapy, especially stem cell therapy, as an alternative promising therapeutic option for the treatment and control of novel COVID-19 and its potential role in tissue rejuvenation after COVID-19 infection.

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Mesenchymal Stem Cells Enhance Immune Response and Protectmice Against Lethal Herpes Viral Infection

Cited by 5 publications

References 22 publications

Mesenchymal Stem Cells Can Both Enhance and Inhibit the Cellular Response to DNA Immunization by Genes of Nonstructural Proteins of the Hepatitis C Virus

Mesenchymal Stem Cells Can Both Enhance and Inhibit the Cellular Response to DNA Immunization by Genes of Nonstructural Proteins of the Hepatitis C Virus

Preventive Vaccination with Mesenchymal Stem Cells Protects Mice from Lethal Infection Caused by Herpes Simplex Virus 1

Cell Therapy as an Alternative approach for COVID-19 Infection Consequences: A Non-Systematic Review

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