Mesenchymal stem cells enhance GABAergic transmission in co-cultured hippocampal neurons

Mauri, Mario; Lentini, D; Gravati, Marta; Foudah, Dana; Biella, Gerardo; Costa, Barbara; Toselli, Mauro; Parenti, Marco; Coco, Silvia

doi:10.1016/j.mcn.2012.02.004

Cited by 28 publications

(27 citation statements)

References 56 publications

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“…Lim et al, 2011;Ma et al, 2012;Makar et al, 2008;Makar et al, 2009). Furthermore, modest endogenous levels of BDNF protein in mesenchymal and neural stem cells (Chekhonin et al, 2011;Li et al, 2006;Mauri et al, 2012) have also been shown to be beneficial on co-cultured neurons (Mauri et al, 2012). Hence, the 8-fold overexpression of functional BDNF-GFP we achieved here is of physiological relevance.…”

Section: Expression Level Of Bdnf-gfp In Escsmentioning

confidence: 69%

“…Interestingly, mesenchymal stem cells (MSCs) can be epigenetically modified to endogenously express modest levels of BDNF (Somoza et al, 2010). Furthermore, it has been shown that, in a co-culture system with hippocampal neurons, MSCs induce glial-dependent neuronal survival, presumably through the secretion of BDNF (Mauri et al, 2012). Because BDNF enhances differentiation, these stem cells potentially show the capacity to support their own differentiation in an autocrine fashion.…”

Section: Introductionmentioning

confidence: 99%

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Stably BDNF-GFP expressing embryonic stem cells exhibit a BDNF release-dependent enhancement of neuronal differentiation

Leschik

Eckenstaler

Nieweg

et al. 2013

Journal of Cell Science

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SummaryBrain-derived neurotrophic factor (BDNF) is known to be a crucial regulator of neuronal survival and synaptic plasticity in the mammalian brain. Furthermore, BDNF positively influences differentiation of embryonic neural precursors, as well as that of neural stem cells from adult neurogenic niches. To study the impact of cell-released BDNF on neural differentiation of embryonic stem cells (ESCs), which represent an attractive source for cell transplantation studies, we have generated mouse ESC clones overexpressing BDNF-GFP by use of knock-in technology. After neural differentiation in vitro, we observed that ESC clones overexpressing BDNF-GFP gave rise to an increased number of neurons as compared to control ESCs. Neurons derived from BDNF-GFP-expressing ESCs harbored a more complex dendritic morphology and differentiated into the GABAergic lineage more than controls. Moreover, we show that ESC-derived neurons released BDNF-GFP in an activity-dependent manner and displayed similar electrophysiological properties as cortical neurons. Thus, our study describes the generation of ESCs stably overexpressing BDNF-GFP, which are ideally suited to investigate the ameliorating effects of BDNF in cell transplantation studies of various neuropathological conditions.

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Section: Expression Level Of Bdnf-gfp In Escsmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Stably BDNF-GFP expressing embryonic stem cells exhibit a BDNF release-dependent enhancement of neuronal differentiation

Leschik

Eckenstaler

Nieweg

et al. 2013

Journal of Cell Science

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“…Cells were visualized using an inverted microscope (Nikon TE 200) and the signals were recorded as described previously (Onorati et al, 2011). Ligand-gated currents, elicited by the local application of GABA (20 M), and GABAergic spontaneous postsynaptic currents (sPSCs) were derived as described (Mauri et al, 2012). To study dopaminergic modulation of GABA-elicited currents, 100 M dopamine was co-applied with GABA.…”

Section: Electrophysiologymentioning

confidence: 99%

Developmentally coordinated extrinsic signals drive human pluripotent stem cell differentiation toward authentic DARPP-32+ medium-sized spiny neurons

et al. 2013

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SUMMARYMedium-sized spiny neurons (MSNs) are the only neostriatum projection neurons, and their degeneration underlies some of the clinical features of Huntington's disease. Using knowledge of human developmental biology and exposure to key neurodevelopmental molecules, human pluripotent stem (hPS) cells were induced to differentiate into MSNs. In a feeder-free adherent culture, ventral telencephalic specification is induced by BMP/TGF inhibition and subsequent SHH/DKK1 treatment. + MSNs. Similar to mature MSNs, these neurons carry dopamine and A2a receptors, elicit a typical firing pattern and show inhibitory postsynaptic currents, as well as dopamine neuromodulation and synaptic integration ability in vivo. When transplanted into the striatum of quinolinic acid-lesioned rats, hPS-derived neurons survive and differentiate into DARPP-32 + neurons, leading to a restoration of apomorphine-induced rotation behavior. In summary, hPS cells can be efficiently driven to acquire a functional striatal fate using an ontogeny-recapitulating stepwise method that represents a platform for in vitro human developmental neurobiology studies and drug screening approaches.

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“…They are believed to be responsible for growth, wound healing, and replacing cells that are lost Because of these functions, they have been shown to be effective in the treatment of stroke. Due to immunological properties of MSCs, they are now considered to be foremost candidate cells for regenerative therapy especially in neurological disorders [13,14]. In recent studies by Suda et al [15], Zhao et al [16], Kaengkan et al [17] found that combination therapy with neuroprotective agents could enhances the amelioration of ischemic brain damage in rats.…”

Section: Res Med Eng Scimentioning

confidence: 99%

Role of Mesenchymal Stem Cells (MSC) in the Management of Brain Stroke

Teegala¹

2017

RMES

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Cerebro vascular Accident (CVA) also commonly known as brain stroke leads to death of brain cells. There are limitations in the effective management of stroke due to lack of definitive treatment measures. In selective cases, interventional radiological and surgical procedure may reduce or prevent the progression of disease. Medical management mainly aimed at long term prevention of stroke. There are no proven medical or surgical measures to rescue the damaged neurons of CNS origin. Mesenchymal stem cells (MSCs) have emerged as a promising approach for the replacement of lost neurons by multiple unique properties of MSCs like neo angiogenesis, stimulating endogenous neurogenesis, autocrine effect, migration, inflammatory milieu and reducing glial scar formation. MSCs are heterogeneous cells with fibroblast-like nature. They can be isolated from nearly all human tissues, including, but not limited to, placenta, umbilical cord, adipose tissue, bone marrow, skeletal muscle and heart. In this mini review, we summarize importance of Mesenchymal stem cells in the Management of Brain Stroke.

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Mesenchymal stem cells enhance GABAergic transmission in co-cultured hippocampal neurons

Cited by 28 publications

References 56 publications

Stably BDNF-GFP expressing embryonic stem cells exhibit a BDNF release-dependent enhancement of neuronal differentiation

Stably BDNF-GFP expressing embryonic stem cells exhibit a BDNF release-dependent enhancement of neuronal differentiation

Developmentally coordinated extrinsic signals drive human pluripotent stem cell differentiation toward authentic DARPP-32+ medium-sized spiny neurons

Role of Mesenchymal Stem Cells (MSC) in the Management of Brain Stroke

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