Mesenchymal stem cells‐derived extracellular vesicles containing miR‐378a‐3p inhibit the occurrence of inflammatory bowel disease by targeting GATA2

Abstract

This study sought to determine whether mesenchymal stem cells‐derived extracellular vesicles (MSCs‐EVs) carrying microRNA‐378a‐3p (miR‐378a‐3p) could affect the pathogenesis of inflammatory bowel disease (IBD) by regulating the GATA‐binding protein 2 (GATA2)/aquaporin‐4 (AQP4)/peroxisome proliferator‐activated receptor α (PPAR‐α) axis. Initially, colon mucosa biopsy tissues were harvested from healthy controls and patients with IBD for qRT‐PCR and immunohistochemistry analysis. EVs harvested from MSCs and lipopolysaccharide (LPS) were used to stimulate the M064 cells to establish an in vitro inflammation cell model. Besides, 2,4,6‐trinitrobenzene sulfonic acid intracolon administration was performed to establish in vivo IBD mouse models. After loss‐ and gain‐of‐function assays, the regulatory role of MSCs‐derived EVs loaded with manipulated miR‐378a‐3p in IBD in relation to GATA2/AQP4/PPAR‐α were explored. Upregulation of GATA2 was identified in the colon tissue of IBD patients. GATA2, which was a target gene of miR‐378a‐3p, transcriptionally upregulated AQP4. After silencing of GATA2, LPS‐induced apoptosis of M064 cells was reduced by the downregulation of AQP4. Decreased AQP4 contributed to PPAR‐α pathway inactivation and weakened the LPS‐induced apoptosis of M064 cells. MSCs‐EVs delivering miR‐378a‐3p suppressed the GATA2/AQP4/PPAR‐α pathway, which reduced LPS‐induced apoptosis of M064 cells and the occurrence of IBD in mice. Altogether, the current study illustrated that MSCs‐EVs transfer miR‐378a‐3p to reduce the GATA2 expression, which downregulates AQP4 to block the PPAR‐α signalling pathway, thus suppressing the occurrence of IBD.

Comprehensive analysis to identify the influences of SARS-CoV-2 infections to inflammatory bowel disease

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Identification of hub genes and key pathways in pediatric Crohn’s disease using next generation sequencing and bioinformatics analysis

“…Finally, we got hub genes, miRNAs and TFs of CD. CDKN2B [766], hsa-mir-629-5p [767], hsa-mir-146a [768], ESR1 [769], PRDM1 [770] and GATA2 [771] have been discovered to be involved in the CD. CDKN2B [772], hsa-mir-146a [768] and ESR1 [773] have been thought of as a specific and exclusive biomarkers for ulcerative colitis.…”

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“…Transfection with commercial reagents and electroporation‐based techniques are other methods extensively used for loading smaller molecules, such as noncoding RNAs (Izco & Alvarez‐Erviti, 2021, Krishn et al., 2022, Li et al., 2022). Electroporation‐based loading of ITGB6‐targeting siRNA into EVs to deliver it to PC3 cells, specifically downregulated expression of β6 subunit (Izco & Alvarez‐Erviti, 2021).…”