Mesenchymal stem cells conditioned with glucose depletion augments their ability to repair‐infarcted myocardium

Choudhery, Mahmood S; Khan, Mohsin; Mahmood, Ruhma; Mohsin, Sadia; Akhtar, Shamim; Ali, Fatima; Khan, Shaheen N.; Riazuddin, Sheikh

doi:10.1111/j.1582-4934.2012.01568.x

Cited by 53 publications

(51 citation statements)

References 41 publications

(53 reference statements)

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“…The initial and final number of cells were recorded at each passage. The cell doublings and doubling time were calculated [11,12] by use of the following formulas:…”

Section: Cumulative Growth Indexmentioning

confidence: 99%

Effect of mild heat stress on the proliferative and differentiative ability of human mesenchymal stromal cells

et al. 2015

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“…The initial and final number of cells were recorded at each passage. The cell doublings and doubling time were calculated [11,12] by use of the following formulas:…”

Section: Cumulative Growth Indexmentioning

confidence: 99%

Effect of mild heat stress on the proliferative and differentiative ability of human mesenchymal stromal cells

et al. 2015

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“…These parameters have been widely used for assessment of cardiac function previously (Rungwerth et al, 2004;Ou et al, 2011;Choudhery et al, 2012;Leblanc et al, 2013;Mao et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Diazoxide preconditioning of endothelial progenitor cells improves their ability to repair the infarcted myocardium

Mehmood

Muhammad

Khan

et al. 2015

Cell Biology International

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Reduced survival and homing of the transplanted cells in the oxidative stressed ischemic environment limits the potential outcome of cell therapies for myocardial ischemia. Diazoxide (DZ), a highly selective mitochondrial ATP sensitive K(+) channel opener, is known to improve the survival and therapeutic ability of mesenchymal stem cells and skeletal myoblasts for the repair of heart failure. The current study explored the effect of DZ preconditioning in improving the ability of endothelial progenitor cells (EPCs) to counteract, in vitro oxidative stress, and to repair the infarcted myocardium. The EPCs were preconditioned by 30 min incubation with 200 μM DZ followed by exposure to 200 μM hydrogen peroxide (H2 O2 ) for 2 h. Non-preconditioned EPCs with and without exposure to H2 O2 were used as control. DZ preconditioning of EPCs resulted in significantly reduced cell injury as shown by reduced lactate dehydrogenase release and expression of annexin V-PE in comparison to untreated EPCs. Furthermore, DZ preconditioned EPCs exhibited upregulated expression of prosurvival genes (VEGF, SDF-1α, PCNA, and Bcl2 ), improved chemokines release (VEGF, IGF, and SDF-1α), viability, Akt phosphorylation and tube formation. In vivo experiments involved transplantation of DZ preconditioned and untreated EPCs in the left ventricle after permanent ligation of left anterior descending coronary artery in rats. The results demonstrated that DZ EPCs transplanted group showed significant reduction in infarct size along with robust cell proliferation, angiogenesis and improvement in cardiac function. The current study demonstrates that DZ preconditioning enhances EPCs survival under oxidative stress in vitro and their ability to treat myocardial infarction.

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“…High glucose concentrations may also modify the gene expression and direct the differentiation of stem cells to the adipogenic over the chondrogenic and osteogenic lineages (Kearts & Khan 2012). On the other hand, glucose depletion improves these properties due to the overall beneficial effects already described of caloric restriction for stem cells, such as delay of cell death, senescence and ageing (Choudhery et al 2012). These reports suggest that the damage caused by glucose level deregulation may affect some properties of undifferentiated cells in a long-term and on irreversible way (Bastianelli et al 2014).…”

Section: Bmmcs' Proliferationmentioning

confidence: 99%

Isolation, expansion and differentiation of mesenchymal stromal cells from rabbits' bone marrow

et al. 2016

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RESUMO.-[Isolamento, expansão e diferenciação de celulas tronco mesenquimais oriundas da medula óssea de coelhos.] A engenharia de tecidos tem sido uma técnica fundamental no campo da medicina regenerativa, uma vez que permite a criação de peças teciduais tri-dimensionais por meio da associação de células mesenquimais indiferenciadas (ou células estromais mesenquimais -CEMs) e moldes de biomateriais in vitro. Assim, muitos estudos têm sido realizados utilizando estas células oriundas de diferentes espécies animais, e os coelhos são frequentemente utilizados como um modelo animal para estudos in vivo de reparação tecidual. No entanto, a maioria das informações disponíveis sobre a coleta e caracterização de CEMs referem-se às células humanas e murinas, o que traz algumas dúvidas para pesquisadores que desejam trabalhar com coelhos em estudos de reparação de tecidos baseados em CEMs. Neste contexto, o presente estudo objetivou contribuir e aprimorar as informações disponíveis na literatura científica fornecendo uma técnica completa para o isolamento, expansão e diferenciação das MSCs de coelhos. Cé-lulas mononucleares da medula óssea (CMMOs) do úmero e fêmur de coelhos foram obtidas e, para avaliar sua taxa de proliferação, três meios de cultura diferentes foram testadas, aqui referidos como DMEM-P, DMEM'S e α-MEM. As CMMOs também foram cultivadas em meios de indução osteogênico, condrogênico, e linhagens adipogênico para Tissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells -MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and α-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media formulation to provide a better cell proliferation rate with multipotentiality preservation. provar a sua multipotencialidade...

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Mesenchymal stem cells conditioned with glucose depletion augments their ability to repair‐infarcted myocardium

Cited by 53 publications

References 41 publications

Effect of mild heat stress on the proliferative and differentiative ability of human mesenchymal stromal cells

Effect of mild heat stress on the proliferative and differentiative ability of human mesenchymal stromal cells

Diazoxide preconditioning of endothelial progenitor cells improves their ability to repair the infarcted myocardium

Isolation, expansion and differentiation of mesenchymal stromal cells from rabbits' bone marrow

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