Mesenchymal stem cells and nanofibers as scaffolds for the regeneration of thyroid cartilage

Jotz, Geraldo Pereira; Soster, Paula Rigon da Luz; Kunrath, Seno O.; Steffens, Daniela; Braghirolli, Daikelly Iglesias; Zettler, Cláudio Galleano; Beck, Carlos Afonso de Castro; Muccillo, Marcelo de Souza; Lopes, Rui F. F.; Mastella, Bernardo; Pranke, Patrícia

doi:10.1002/lary.24805

Cited by 11 publications

(8 citation statements)

References 15 publications

(11 reference statements)

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“…1,2 Primary laryngeal reconstruction could range from a single cartilage, such as the thyroid cartilage, to the entire larynx; thus knowledge about each laryngeal cartilaginous component is required to enable the development of suitable scaffolding materials to engineer these cartilages. There have been some initial attempts to tissue engineer the hemilarynx, using either decellularized matrix 3 or synthetic materials 1,2,[4][5][6][7][8] but there have been issues with incomplete cellularization and inadequate mechanical properties. Because of the roles of these cartilages in keeping the airway patent, it is critical that the cartilaginous structures, especially the cricoid and thyroid cartilages, are functional immediately upon placement into the patient.…”

Section: Introductionmentioning

confidence: 99%

Mechanical, Cellular, and Proteomic Properties of Laryngotracheal Cartilage

2018

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The larynx sometimes requires repair and reconstruction due to cancer resection, trauma, stenosis, or developmental disruptions. Bioengineering has provided some scaffolding materials and initial attempts at tissue engineering, especially of the trachea, have been made. The critical issues of providing protection, maintaining a patent airway, and controlling swallowing and phonation, require that the regenerated laryngotracheal cartilages must have mechanical and material properties that closely mimic native tissue. These properties are determined by the cellular and proteomic characteristics of these tissues. However, little is known of these properties for these specific cartilages. This review considers what is known and what issues need to be addressed.

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Section: Introductionmentioning

confidence: 99%

Mechanical, Cellular, and Proteomic Properties of Laryngotracheal Cartilage

2018

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“…Exposure to retained molecular cues on the ECM are known to promote cellular attachment and integration (Baiguera et al, ; Conconi et al, ; Sutherland, Baskin, Hayward, & Cunha, ) resulting in a positive outcome for implanted tissue engineered airway graft. Although the use of seeded de‐cellularised scaffolds has been reported favourably in the literature and may be beneficial for large airway replacements (Jotz et al, ), there is no clear consensus. Implantation of nonseeded laryngeal scaffold has also shown tissue regeneration (Grevemeyer et al, ; Kitamura et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…It is hypothesised that seeded cells may act as a “feeder layer” by activating local progenitor cells (Zhang et al, ). Following the creation of a defect in the thyroid cartilage in pigs, cartilaginous neo‐formation could be significantly improved with the application of MSCs (Jotz et al, ). MSCs are commonly used in the treatment of traumatic articular cartilage defect because they enhance the repair of hyaline cartilage (Bornes, Adesida, & Jomha, ).…”

Section: Discussionmentioning

confidence: 99%

“…Whether to seed or not to seed, a scaffold prior to implantation is still under considerable debate, however, the majority of studies favour seeded constructs. For example Jotz et al () compared laryngeal defect closure in a porcine model using a naïve scaffold (nanofibers) and a seeded scaffold (with mesenchymal stem cells, MSCs). The seeded scaffold displayed a significant advantage with the formation of neo‐cartilage.…”

Section: Introductionmentioning

confidence: 99%

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In vivo implantation of a tissue engineered stem cell seeded hemi‐laryngeal replacement maintains airway, phonation, and swallowing in pigs

Herrmann

Ansari

Southgate

et al. 2017

J Tissue Eng Regen Med

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Laryngeal functional impairment relating to swallowing, vocalisation, and respiration can be life changing and devastating for patients. A tissue engineering approach to regenerating vocal folds would represent a significant advantage over current clinical practice. Porcine hemi-larynx were de-cellularised under negative pressure. The resultant acellular scaffold was seeded with human bone marrow derived mesenchymal stem cells and primary human epithelial cells. Seeded scaffolds were implanted orthotopically into a defect created in the thyroid cartilage in 8 pigs and monitored in vivo for 2 months. In vivo assessments consisted of mucosal brushing and bronchoscopy at 1, 2, 4, and 8 weeks post implantation followed by histological evaluation post termination. The implanted graft had no adverse effect on respiratory function in 6 of the 8 pigs; none of the pigs had problems with swallowing or vocalisation. Six out of the 8 animals survived to the planned termination date; 2 animals were terminated due to mild stenosis and deep tissue abscess formation, respectively. Human epithelial cells from mucosal brushings could only be identified at Weeks 1 and 4. The explanted tissue showed complete epithelialisation of the mucosal surface and the development of rudimentary vocal folds. However, there was no evidence of cartilage remodelling at the relatively early censor point. Single stage partial laryngeal replacement is a safe surgical procedure. Replacement with a tissue engineered laryngeal graft as a single procedure is surgically feasible and results in appropriate mucosal coverage and rudimentary vocal fold development.

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“…Whether to seeding remains controversial; however, the majority of research has demonstrated partiality toward seeded scaffolds. Jotz et al reported that MSC scaffolds demonstrated a significant advantage in forming laryngeal neo‐cartilage in a porcine model 61 . The seeded cells may act as a “feeder layer” by activating local progenitor cells and accelerating the process of tissue integration 62 .…”

Section: Future Workmentioning

confidence: 99%

Tissue engineering of the larynx: A contemporary review

Chen¹,

Shen²,

Shen³

et al. 2020

Clinical Laboratory Analysis

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Objective Tissue engineering has been a topic of extensive research in recent years and has been applied to the regeneration and restoration of many organs including the larynx. Currently, research investigating tissue engineering of the larynx is either ongoing or in the preclinical trial stage. Methods A literature search was performed on the Advanced search field of PubMed using the keywords: “(laryncheal tissue engineering) AND (cartilage regeneration OR scaffolds OR stem cells OR biomolecules).” After applying the selection criteria, 65 articles were included in the study. Results The present review focuses on the rapidly expanding field of tissue‐engineered larynx, which aims to provide stem cell–based scaffolds combined with biological active factors such as growth factors for larynx reconstruction and regeneration. The trend in recent studies is to use new techniques for scaffold construction, such as 3D printing, are developed. All of these strategies have been instrumental in guiding optimization of the tissue‐engineered larynx, leading to a level of clinical induction beyond the in vivo animal experimental phase. Conclusions This review summarizes the current progress and outlines the necessary basic components of regenerative laryngeal medicine in preclinical fields. Finally, it considers the design of scaffolds, support of growth factors, and cell therapies toward potential clinical application.

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Mesenchymal stem cells and nanofibers as scaffolds for the regeneration of thyroid cartilage

Abstract: N/A.

Cited by 11 publications

References 15 publications

Mechanical, Cellular, and Proteomic Properties of Laryngotracheal Cartilage

Mechanical, Cellular, and Proteomic Properties of Laryngotracheal Cartilage

In vivo implantation of a tissue engineered stem cell seeded hemi‐laryngeal replacement maintains airway, phonation, and swallowing in pigs

Tissue engineering of the larynx: A contemporary review

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