Mesenchymal stem cell transplantation to the mouse cochlea as a treatment for childhood sensorineural hearing loss

Kasagi, Hiromi; Kuhara, Takatoshi; Okada, Hiroko; Sueyoshi, Noriyoshi; Kurihara, Hidetake

doi:10.1016/j.ijporl.2013.03.011

Cited by 32 publications

(23 citation statements)

References 33 publications

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“…To the best of our knowledge, this is the first study that has been undertaken to detect luciferase-expressing cells in the intact cochlea of guinea pigs using bioluminescence imaging. Although other authors have tracked greenfluorescent stem cells in histological sections of the cochlea from various rodent species using fluorescence microscopy (Regala et al, 2005;Hu and Ulfendahl, 2006;Sekiya et al, 2006;Bogaerts et al, 2008;Ogita et al, 2009;Kasagi et al, 2013), the present study is the first that uses molecular optical imaging for the non-destructive in situ detection of bioluminescent stem cells injected into an anatomically complex structure such as the cochlea.…”

Section: Discussionmentioning

confidence: 98%

Imaging Bioluminescent Exogenous Stem Cells in the Intact Guinea Pig Cochlea

Schomann

Mezzanotte²,

Groot

et al. 2019

The Anatomical Record

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Stem‐cell‐based therapy may be used to replace damaged or lost neurons in the cochlear nerve of patients suffering from severe‐to‐profound sensorineural hearing loss. In order to achieve functional recovery in future clinical trials, knowledge about survival of grafted cells and their differentiation into functional neurons is a prerequisite. This calls for non‐invasive in vivo visualization of cells and long‐term monitoring of their survival and fate after cochlear transplantation. We have investigated if molecular optical imaging enables visualization of exogenous cells in the intact cochlea of guinea pig cadaver heads. Transduced (stem) cells, stably co‐expressing fluorescent (copGFP) and bioluminescent (Luc2) reporter molecules, were injected into the internal auditory meatus or directly into the cochlea through the round window. After injection of the cells into the internal auditory meatus, a bright bioluminescent signal was observed in the cavum conchae of the auricle, indicating that light generated by Luc2 is passing through the tympanic membrane and the external auditory meatus. Similar results were obtained after injection of the cells through the round window membrane, either directly into the scala tympani or in Rosenthal's canal within the modiolus of the basal cochlear turn. Imaging of the auditory bulla demonstrated that the bioluminescent signal passes through the tympanic membrane and crevices in the bony wall of the bulla. After opening the auditory bulla, the bioluminescent signal was emanating from the round window. This is the first study demonstrating that bioluminescence imaging enables visualization of luciferase‐expressing cells injected into the intact guinea pig cochlea. Anat Rec, 303:427–440, 2020. © 2019 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.

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Section: Discussionmentioning

confidence: 98%

Imaging Bioluminescent Exogenous Stem Cells in the Intact Guinea Pig Cochlea

Schomann

Mezzanotte²,

Groot

et al. 2019

The Anatomical Record

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“…However, Zheng et al [67] isolated cochlear SCs from newborn rats and transplanted them into the scala tympani of gentamicindeafened rats, showing a migration of transplanted HC-like cells from the injection site to the basal membrane and organ of Corti with hearing recovery. Other studies showed that transplanted SCs could survive at the injection site, regardless of the presence of an external acoustic damage [68][69][70]. Kasagi et al [68] investigated the ability of young and old mice to accept mouse BM-MSC transplantation into the perilymph, showing a positive engraftment only in young individuals, with no adverse effects on auditory brainstem response (ABR) test in both groups.…”

Section: In Vivo Regeneration By Scsmentioning

confidence: 99%

Regenerative medicine in hearing recovery

Simoni

Orsini²,

Chicca

et al. 2017

Cytotherapy

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Hearing loss, or deafness, affects 360 million people worldwide of which about 32 million are children. Deafness is irreversible when it involves sensory hair cell death because the regenerative ability of these cells is lost in mammals after embryo development. The therapeutic strategies for deafness include hearing aids and/or implantable devices. However, not all patients are eligible or truly benefit from these medical devices. Regenerative medicine based on stem cell application could play a role in both improvement of extant medical devices and in vivo recovery of auditory function by regeneration of inner ear cells and neurons. A review of recent literature on the subject indicates that two promising approaches to renewal and differentiation of cochlear tissues are transplantation of stem cells and in situ administration of growth factors. Rather than directly regenerating dead cells, these procedures apparently induce, through various pathways, differentiation of resident cochlear cells. More studies on the possible adverse effects of transplanted cells and the recovery of tonotopic sensorineural activity or required. To date, no reliable clinical results have been obtained in the field of cochlear regeneration.

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“…The BMSCs have been fruitfully transplanted into young mice cochlea via the perilymphatic perfusion technique and further differentiated into fibrocyte-like (SLFlike) cells 35 . The hearing recovery after transplantation of MSCs into the inner ear cells may be occurred through two mechanisms.…”

Section: Hearing Recovery After Transplantation Of Mscsmentioning

confidence: 99%

Comparison of Three Types of Mesenchymal Stem Cells (Bone Marrow, Adipose Tissue, and Umbilical Cord-Derived) as Potential Sources for Inner Ear Regeneration

Mahmoudian-Sani

Mehri-Ghahfarrokhi

Hashemzadeh‐Chaleshtori

et al. 2017

The International Tinnitus Journal

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In this review, we compared the potential of mesenchymal stem cells derived from bone marrow, adipose tissue and umbilical cord as suitable sources for regeneration of inner ear hair cells and auditory neurons. Our intensive literature search indicates that stem cells in some of adult mammalian tissues, such as bone marrow, can generate new cells under physiological and pathological conditions. Among various types of stem cells, bone marrow-derived mesenchymal stem cells are one of the most promising candidates for cell replacement therapy. Mesenchymal stem cells have been reported to invade the damaged area, contribute to the structural reorganization of the damaged cochlea and improve incomplete hearing recovery. We suggest that bone marrow-derived mesenchymal stem cells would be more beneficial than other mesenchymal stem cells.

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Mesenchymal stem cell transplantation to the mouse cochlea as a treatment for childhood sensorineural hearing loss

Cited by 32 publications

References 33 publications

Imaging Bioluminescent Exogenous Stem Cells in the Intact Guinea Pig Cochlea

Imaging Bioluminescent Exogenous Stem Cells in the Intact Guinea Pig Cochlea

Regenerative medicine in hearing recovery

Comparison of Three Types of Mesenchymal Stem Cells (Bone Marrow, Adipose Tissue, and Umbilical Cord-Derived) as Potential Sources for Inner Ear Regeneration

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