Mesenchymal Stem Cell Transition to Tumor-Associated Stromal Cells Contributes to Cancer Progression

Bussard, Karen M.; Spaeth, Erika L.; Mutkus, Lysette; Stumpf, Kristina; Marini, Frank C.

doi:10.1016/b978-0-12-803102-5.00011-2

Cited by 4 publications

(1 citation statement)

References 89 publications

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“…CD144 is an endothelium-specific member of the cadherin family called VE-cadherin and belongs to the adherens junctions molecules [ 18 ]. CD146 is an endothelial cell–cell adhesion molecule as well expressed in the vascular system [ 19 , 20 ]. All tree endothelial adhesion molecules investigated in here CD31, CD144, and CD146, regulate leukocyte trafficking and vascular permeability.…”

Section: Resultsmentioning

confidence: 99%

Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human

Bienert

Habib

Buck

et al. 2021

Arch Gynecol Obstet

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Endometrial receptivity is a decisive factor in human reproduction. Human chorionic gonadotropin (hCG) is one of the first embryonic signals that precedes the implantation by trophoblast invasion into the endometrium. Meta-analysis of randomized controlled trials reports a moderate-quality evidence for improved live birth rate for an intrauterine hCG dose ≥ 500 IU. Nevertheless, all hCG endometrial effects are not completely understood. We, therefore, utilized endometrial tissue from 12 patients after estradiol and progesterone treatment with or without intrauterine hCG flushing at the window of implantation (WOI) to analyze cellular composition by measuring marker proteins for stromal, endothelial, epithelial and immune cells. Flow cytometry analysis revealed that significantly more cells expressed the endothelial adhesion molecules VE-cadherin (CD144) and S-Endo-1 (CD146) after intrauterine hCG administration. In contrast, the endothelial marker CD31 and markers involved in vessel formation (VEGFR1 and VEGFR2) remained unchanged in their expression. Similarly, stroma markers (CD73, CD90 and CD105), epithelial markers (Desmocollin-2 and E-Cadherin) and immune cell markers (CD11b, CD45, CD79a and HLA-DR) displayed no alterations in their expression. This finding directs the focus on endothelial adhesion molecules as a potential mechanistically explanation of hCG conveyed increase of embryo implantation and pregnancy rates in women undergoing ART.

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Section: Resultsmentioning

confidence: 99%

Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human

Bienert

Habib

Buck

et al. 2021

Arch Gynecol Obstet

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Co‐culturing multicellular tumor models: Modeling the tumor microenvironment and analysis techniques

2021

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Advances in two-dimensional (2D) and three-dimensional (3D) cell culture over the last 10 years have led to the development of a plethora of methods for cultivating tumor models. More recently, cellular co-cultures have become a suitable testbed. The first portion of this review focuses on co-culturing methods that have been developed in recent years utilizing the multicellular tumor spheroid model. The latter portion describes techniques that are used to analyze the proteomes of mono-or co-cultured tumor models, with a focus on mass spectrometry (MS)-based analyses. Protein profiles are important indicators of the tumor heterogeneity. Therefore, there is a specific focus within this review on analysis by MS and MS imaging methods evaluating the proteomic profiles of 2D and 3D co-cultures. While these models are incredibly important for biological research, so far, they have not been widely explored on the proteomic level. With this review, we aim to introduce these systems to an analytical audience, with the goal of highlighting MS as an underutilized tool for proteomic analysis of tumor models.

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Osteosarcoma cells exhibit functional interactions with stromal cells, fostering a lung microenvironment conducive to the establishment of metastatic tumor cells

Valenzuela Alvarez,

Gutierrez,

Bayo

et al. 2024

Mol Biol Rep

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Mesenchymal Stem Cell Transition to Tumor-Associated Stromal Cells Contributes to Cancer Progression

Cited by 4 publications

References 89 publications

Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human

Intrauterine hCG application increases expression of endothelial cell–cell adhesion molecules in human

Co‐culturing multicellular tumor models: Modeling the tumor microenvironment and analysis techniques

Osteosarcoma cells exhibit functional interactions with stromal cells, fostering a lung microenvironment conducive to the establishment of metastatic tumor cells

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