Mesenchymal stem cell response to UV-photofunctionalized TiO<sub>2</sub>coated CoCrMo

Logan, Niall; Cross, Alison J.; Traynor, Alison; Bozec, Laurent; Parkin, Ivan P.; Brett, Peter

doi:10.1039/c4ra11524d

Cited by 3 publications

(8 citation statements)

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“…These supplements trigger the MSCs to differentiate along their osteogenic linage to form osteoblasts and as a consequence can reduce proliferation. 19,34 Cell attachment was analyzed after 24 h in GM. It was found that significantly more cells had attached to SMO and AE compared to SLA50 and SLA250 (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Discs formed of CoCrMo (Cr 26–30%, Mo 5–7%), dimensions of Ø 15 mm, 1 mm thickness, were supplied with a machined finish which was removed as previously described to create a smooth (SMO) topography . SMO discs were then used to engineer three other topographies; acid etched (AE), sand blasted acid etched using 50 µm Al 2 O 3 grit (SLA50), and sand blasted acid etched using 250 µm Al 2 O 3 (SLA250).…”

Section: Methodsmentioning

confidence: 99%

“…Discs formed of CoCrMo (Cr 26-30%, Mo 5-7%), dimensions of Ø 15 mm, 1 mm thickness, were supplied with a machined finish which was removed as previously described to create a smooth (SMO) topography. 17,19 SMO discs were then used to engineer three other topographies; acid etched (AE), sand blasted acid etched using 50 mm Al 2 O 3 grit (SLA50), and sand blasted acid etched using 250 mm Al 2 O 3 (SLA250). AE substrates were created by following a previously reported protocol, 24 which involved acid etching in HCl 7.4% and H 2 SO 4 76% for 6 min at 1008C, followed by HNO 3 30% for 5 min at 608C.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Cobalt chromium molybdenum (CoCrMo) lacks the bioactivity of titanium, but is widely used in orthopedic applications where a mechanically superior material is required. Efforts to improve the bioactivity of CoCrMo have included chemical vapor deposition coating, sol–gel coating, ultraviolet photofunctionalization, and immobilization with bone morphogenic peptides . Surface modification of CoCrMo through topographical methods remains largely un‐investigated.…”

Section: Introductionmentioning

confidence: 99%

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Mesenchymal stem cell response to topographically modified CoCrMo

Logan

Bozec

Traynor

et al. 2015

J Biomedical Materials Res

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Surface roughness on implant materials has been shown to be highly influential on the behavior of osteogenic cells. Four surface topographies were engineered on cobalt chromium molybdenum (CoCrMo) in order to examine this influence on human mesenchymal stem cells (MSC). These treatments were smooth polished (SMO), acid etched (AE) using HCl 7.4% and H2SO4 76% followed by HNO3 30%, sand blasted, and acid etched using either 50 μm Al2O3 (SLA50) or 250 μm Al2O3 grit (SLA250). Characterization of the surfaces included energy dispersive X‐ray analysis (EDX), contact angle, and surface roughness analysis. Human MSCs were cultured onto the four CoCrMo substrates and markers of cell attachment, retention, proliferation, cytotoxicity, and osteogenic differentiation were studied. Residual aluminum was observed on both SLA surfaces although this appeared to be more widely spread on SLA50, whilst SLA250 was shown to have the roughest topography with an R a value greater than 1 μm. All substrates were shown to be largely non‐cytotoxic although both SLA surfaces were shown to reduce cell attachment, whilst SLA50 also delayed cell proliferation. In contrast, SLA250 stimulated a good rate of proliferation resulting in the largest cell population by day 21. In addition, SLA250 stimulated enhanced cell retention, calcium deposition, and hydroxyapatite formation compared to SMO (p < 0.05). The enhanced response stimulated by SLA250 surface modification may prove advantageous for increasing the bioactivity of implants formed of CoCrMo. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3747–3756, 2015.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mesenchymal stem cell response to topographically modified CoCrMo

Logan

Bozec

Traynor

et al. 2015

J Biomedical Materials Res

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“…collagen type I. Furthermore, surface hydrophilicity enhances cell interaction and adhesion to dental implants (Zhao et al, 2005, Logan et al, 2014a). …”

Section: Introductionmentioning

confidence: 99%

Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro

et al. 2017

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Since the 1980s, titanium (Ti) implants have been routinely used to replace missing teeth. This success is mainly due to the good biocompatibility of Ti and the phenomenon of osseointegration, with very early events at implant placement being important in determining good osseointegration. However, enhancing implant performance with coatings such as hydroxyapatite (HA) and calcium phosphate has proved largely unsuccessful. Human mesenchymal stem cells (hMSCs) are the first osteogenic cells to colonise implant surfaces and offer a target for enhancing osseointegration. We previously reported that small doses of bisphosphonate (BP) may play an integral role in enhancing hMSC proliferation and osteogenic differentiation. The aim of this study is to investigate whether small doses of bisphosphonates enhance proliferation and osteogenic differentiation of hMSCs on Ti surfaces, to enhance bone osseointegration and to accelerate wound healing around the implant surface. Our data suggests that treating cells with small doses of BP (100 nM & 10 nM) induces significant hMSC stimulation of osteogenic markers including calcium, collagen type I and ALP compared to control group on titanium surfaces (P < 0.05). In addition, cell proliferation and migration were significantly enhanced on titanium surfaces (P < 0.05).

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The Effect of Microcosm Biofilm Decontamination on Surface Topography, Chemistry, and Biocompatibility Dynamics of Implant Titanium Surfaces

Sousa

Mardas

Spratt

et al. 2022

IJMS

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Since the inception of dental implants, a steadily increasing prevalence of peri-implantitis has been documented. Irrespective of the treatment protocol applied for the management of peri-implantitis, this biofilm-associated pathology, continues to be a clinical challenge yielding unpredictable and variable levels of resolution, and in some cases resulting in implant loss. This paper investigated the effect of microcosm biofilm in vitro decontamination on surface topography, wettability, chemistry, and biocompatibility, following decontamination protocols applied to previously infected implant titanium (Ti) surfaces, both micro-rough -Sandblasted, Large-grit, Acid-etched (SLA)-and smooth surfaces -Machined (M). Microcosm biofilms were grown on SLA and M Ti discs. These were treated with TiBrushes (TiB), combination of TiB and photodynamic therapy (PDT), combination of TiB and 0.2%CHX/1%NaClO, plus or minus Ultraviolet-C (UV-C) radiation. Surface topography was evaluated by Scanning Electron Microscopy (SEM) and Laser Surface Profilometry. Surface function was analysed through wettability analysis. Surface chemistry evaluation of the discs was performed under SEM/Energy-dispersive X-ray spectroscopy (EDX) and X-ray photoelectron spectroscopy (XPS). Biocompatibility was tested with the cytocompatibility assay using human osteoblast-like osteosarcoma cell line (MG-63) cells. Elemental analysis of the discs disclosed chemical surface alterations resulting from the different treatment modalities. Titanium, carbon, oxygen, sodium, aluminium, silver, were identified by EDX as the main components of all the discs. Based on the data drawn from this study, we have shown that following the decontamination of Ti surfaces the biomaterial surface chemistry and topography was altered. The type of treatment and Ti surface had a significant effect on cytocompatibility (p = 0.0001). Although, no treatment modality hindered the titanium surface biocompatibility, parameters such as the use of chemical agents and micro-rough surfaces had a higher cytotoxic effect in MG-63 cells. The use of smooth surfaces, and photofunctionalisation of the TiO2 layer had a beneficial effect on cytocompatibility following decontamination.

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Mesenchymal stem cell response to UV-photofunctionalized TiO₂coated CoCrMo

Abstract: UV photofunctionalization of TiO2 coated implant surfaces enhanced markers of cell adhesion. This may prove to be advantageous for orthopaedic implants by supporting the colonisation and adhesion of cells capable of facilitating in bone healing.

Cited by 3 publications

References 43 publications

Mesenchymal stem cell response to topographically modified CoCrMo

Mesenchymal stem cell response to topographically modified CoCrMo

Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro

The Effect of Microcosm Biofilm Decontamination on Surface Topography, Chemistry, and Biocompatibility Dynamics of Implant Titanium Surfaces

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Mesenchymal stem cell response to UV-photofunctionalized TiO2coated CoCrMo

Abstract: UV photofunctionalization of TiO2 coated implant surfaces enhanced markers of cell adhesion. This may prove to be advantageous for orthopaedic implants by supporting the colonisation and adhesion of cells capable of facilitating in bone healing.

Cited by 3 publications

References 43 publications

Mesenchymal stem cell response to topographically modified CoCrMo

Mesenchymal stem cell response to topographically modified CoCrMo

Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro

The Effect of Microcosm Biofilm Decontamination on Surface Topography, Chemistry, and Biocompatibility Dynamics of Implant Titanium Surfaces

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Product

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Mesenchymal stem cell response to UV-photofunctionalized TiO₂coated CoCrMo