Mesenchymal Stem Cell Properties of Periodontal Ligament Cells From Deciduous and Permanent Teeth

Abstract

These findings demonstrate that highly purified mesenchymal progenitor cell subsets can be obtained from the PDLs of both deciduous and permanent teeth, and further indicate phenotype dissimilarities that may have an impact on their clinical applications.

Osteogenic potential of periodontal ligament stem cells are unaffected after exposure to lipopolysaccharides

“…The PDL progenitor cell populations were previously characterized by our group as being pluripotent mesenchymal progenitor cells by expression of stem cell markers such as CD105, CD166, OCT-4, and STRO-1. 11 Studies have shown that the TLRs family is involved in the recognition of bacterial cell wall components 20 and a current study described that PgLPS triggers an inflammatory response by binding to TLR2 and TLR4. 10 In the present study, TLR2 expression in PDLSC populations was verified, emphasizing that these cells can recognize bacterial virulence factors.…”

“…Three populations of mesenchymal progenitor cells (STRO1 + , CD105 + , CD34 -, and CD45 -cells) from periodontal ligament (PDL) of permanent teeth were obtained and characterized in a previous study. 11 Briefly, CD105 + -enriched cell subsets from PDL were isolated by magnetic cell sorting, and characterized by flow cytometry and immunostaining. All three PDLSC populations were cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM) supplemented with 10% fetal bovine serum, 1% L glutamine and 1% penicillin/streptomycin (GIBCO BRL, Life Technologies, Carlsbad, USA) (standard media) at 37 o C in atmosphere containing 5% CO 2 , frozen with Recovery™ Cell Culture Freezing Medium (Gibco BRL) and kept in liquid nitrogen for subsequent experiments.…”

Tooth-derived stem cells: Update and perspectives

“…PDLSCs are more proliferative and clonogenic than BMSCs [34] . PDLSCs can also differentiate into adipocytes [34,73] and chondrocytes [34] , as well as osteoblasts/cementoblasts [34,60,73] . Although PDLSCs are able to form mineralized tissue in vitro when osteoblastic/cementoblastic differentiation is induced, they form fewer mineralized nodules than BMSCs [34,73] .…”

“…Subsequently, several studies were performed to identify other sources and to understand how these cells can give rise to distinct cell types, for the purpose of using these cells in regenerative procedures [39][40][41][42][43] . In this context, dental tissues have also been investigated as niches of MSCs, and many toothderived SCs (TDSCs) have been identified and characterized, including dental pulp SCs (DPSCs) [44][45][46][47][48] , SCs from human exfoliated deciduous teeth (SHED) [49][50][51][52][53] , periodontal ligament SCs (PDLSCs), dental follicle progenitor cells (DFPCs) [54][55][56] , SCs from apical papilla (SCAP) [19,[56][57][58][59] , and periodontal ligament of deciduous teeth SCs (DePDL) [50,51,[60][61][62] (Figure 1). Dental tissues are an accessible source of MSCs that can be obtained with limited morbidity and without additional risks to the donor, as extracted/exfoliated teeth represent a waste product of dental procedures [13,[63][64][65] .…”

Stem Cells in Deciduous Teeth – Their Potential use in Medicine

“…Besides SHED, there are also periodontal ligament cells from deciduous teeth (dePDLSCs) that have been isolated and characterized. Compared to the same cell type from permanent teeth (pePDLSCs), both are typical MSCs having similar characteristics [17]. dePDLSCs appear to be more proliferative and osteogenic while pePDLSCs secrete more neurotropic factors [18,19].…”