Mesenchymal Stem Cell Population Isolated from the Subepithelial Layer of Umbilical Cord Tissue

Abstract

The therapeutic use of stem cells to treat diseases and injuries is a promising tool in regenerative medicine. The umbilical cord provides a rich source of stem cells; we have previously reported a population of stem cells isolated from Wharton's jelly. In this report, we aimed to isolate a novel cell population that was different than those found in Wharton's jelly. We isolated stem cells from the subepithelial layer of the umbilical cord; the cells could be expanded for greater than 90 population doubling and had mesenchymal stem cell characteristics, expressing CD9, SSEA4, CD44, CD90, CD166, CD73, and CD146 but were negative for STRO-1. The cells can be directionally differentiated and undergo osteo-, chondro-, adipo-, and cardiogenesis. In addition, we have identified for the first time that mesenchymal stem cells isolated from umbilical cord can produce microvesicles, termed exosomes. This is the first report describing a stem cell population isolated from the subepithelial layer of the umbilical cord. Given the growth capacity, multilineage potential, and most importantly the low levels of HLA-ABC, we propose that this novel cell isolated from the subepithelial layer of umbilical cord is an ideal candidate for allogeneic cell-based therapy.

Delivery of the Sox9 gene promotes chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells in an in vitro model

“…Although bone marrow is the main source, MSCs have already been isolated from various other tissues, such as adipose tissue (22) and umbilical cord (23). Choudhery et al (24) reported that confluent cultures of MSCs either from adipose tissue or cord tissue show a fibroblastic morphology.…”

Mouse Dental Pulp Stem Cells Support Human Umbilical Cord Blood-Derived Hematopoietic Stem/Progenitor Cells in Vitro

“…Many investigators have since reported the establishment of MSC-like cells from a variety of fetal (29) and adult mesenchymal tissues, including nonhematopoietic tissues (31,32,34,36). The potential of MSC-like cells isolated from nonhematopoietic tissues to support HSCs still remains unclear, while the HSC-supporting potential of BM-MSCs is well documented.…”

“…Hematopoietic stem cells (HSCs) can self-renew and differentiate into all types of blood cells (2,14,29). These HSCs are maintained in an undifferentiated state in a specialized environment, which is called the HSC niche (17,19).…”

Stable cell adhesion affects mesenchymal stem cell sheet fabrication: Effects of fetal bovine serum and human platelet lysate

“…Banked human umbilical cord mesenchymal stem cells (hUC‐MSCs; Jadi Cell LLC, Miami, USA; Patel, Vargas, Revello, & Bull, 2013) were cultured in cell culture media with Dulbecco's Modified Eagle's Medium (DMEM; Life Technologies, USA) supplemented with either 10% hPL (Jadi Cell LLC) or 10% FBS (Life Technologies), 1% Glutamax (Life Technologies), 1% MEM NEAA (Life Technologies), 1% penicillin streptomycin (Life Technologies), at 37°C in a humidified atmosphere with 5% CO 2 from passage 2 (P2) to P12. Cell culture media was changed every 2 days.…”