Mesenchymal Stem Cell-Cardiomyocyte Interactions under Defined Contact Modes on Laser-Patterned Biochips

Ma, Zhen; Yang, Huaxiao; Liu, Honghai; Xu, Meifeng; Runyan, Raymond B.; Eisenberg, Carol A.; Markwald, Roger R.; Borg, Thomas K.; Gao, Bruce Z.

doi:10.1371/journal.pone.0056554

Cited by 37 publications

(22 citation statements)

References 48 publications

(52 reference statements)

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“…We report increased gene expression levels in CMWs of key cardiac maturation markers, including genes implicated in sarcomere structure, as well BNP, which increases during the fetal heart gene program when organogenesis commences (40). We speculate that there may be high expression of integrins and adhesion proteins in the CD90+ cells that promotes ECM remodeling and, as a direct result, bring cells together during tissue contraction to promote cell-cell contact, which has been shown to facilitate maturation signaling (41)(42)(43). Putatively fibroblasts, these CD90+ cells may also secrete growth factors such as bFGF and VEGF.…”

Section: Discussionmentioning

confidence: 83%

Design and formulation of functional pluripotent stem cell-derived cardiac microtissues

Thavandiran

Dubois

Mikryukov

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

254

248

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Significance Robust and predictive in vitro models of human cardiac tissue function could have transformative impact on our ability to test new drugs and understand cardiac disease. Despite significant effort, the generation of high-fidelity adult-like human cardiac tissue analogs remains challenging. In this paper, we systematically explore the design criteria for pluripotent stem cell-derived engineered cardiac tissue. Parameters such as biomechanical stress during tissue remodeling, input-cell composition, electrical stimulation, and tissue geometry are evaluated. Our results suggest that a specified combination of a 3D matrix-based microenvironment, uniaxial mechanical stress, and mixtures of cardiomyocytes and fibroblasts improves the performance and maturation state of in vitro engineered cardiac tissue.

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Section: Discussionmentioning

confidence: 83%

Design and formulation of functional pluripotent stem cell-derived cardiac microtissues

Thavandiran

Dubois

Mikryukov

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

254

248

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“…The use of this method provided unique information in modern cell biology. For instance, bivalent dumbbell-shaped cells, inductors of new subpopulation of pluripotent cells, and initiators of new immune memory T cells were thus identifi ed [6,8,21,23,28,32]. The dynamics of cell migration and 3D-interactions that allow histogenesis programming in the spheres of through of E-cadherin clusters and axial actin networks were studied [7,18].…”

mentioning

confidence: 99%

3D-Technology of the Formation and Maintenance of Single Dormant Microspheres from 2000 Human Somatic Cells and Their Reactivation In Vitro

et al. 2014

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We developed an original reproducible 3D-technology for preparation of single dormant microspheres consisting of 2000 somatic cells. The dynamics of microsphere assembly from mesenchymal and epithelial cells of retinal pigment epithelium was traced using time-lapse microscopy: formation of a loose aggregate over 24 h followed by its gradual consolidation and formation of a compact viable microsphere with a diameter of 100-150 μ by day 7. The cell number in the formed microspheres remains unchanged. Reactivation observed upon fusion of epithelial and/or mesenchymal microspheres results in the formation of a united compact microtissue. The fusion dynamics reproduces spherogenesis irrespective of the initial amount of co-cultured microspheres. Reactivation via two-step induced angiogenesis opens new prospects for production of vascularized microspheres and microtissues.

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“…Neonatal CMs were isolated from 3-day-old SD rats using the two-day protocol we previously reported (16). In brief, ten neonatal rats were dissected, and the hearts were collected and minced in Moscona's saline.…”

Section: Methodsmentioning

confidence: 99%

Interactive relationship between basement-membrane development and sarcomerogenesis in single cardiomyocytes

Yang

Borg

Liu

et al. 2015

Experimental Cell Research

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The cardiac basement membrane (BM), the highly organized layer of the extracellular matrix (ECM) on the external side of the sarcolemma, is mainly composed of laminin and collagen IV, which assemble a dense, well-organized network to surround the surface of each adult cardiomyocyte. The development of the cardiac BM plays a key role in organogenesis of the myocardium through interactions between sarcomeres and integrins. Because of the complicated structure of cardiac muscle fibers and lack of a proper investigation method, the detailed interactions among BM development, sarcomeric growth, and integrin expression remain unclear. In this study, freshly isolated 3-day neonatal cardiomyocytes (CMs) were cultured on aligned collagen, which mimics the in vivo ECM structure and induces neonatal CMs to grow into rod-like shapes. Then double fluorescence-immunostained laminin and α-actinin or integrin β1 on neonatal CMs cultured 4-72 h were imaged using a confocal microscope, and the spatial relationship between laminin deposition and α-actinin expression was evaluated by colocalization analysis. At 4 h, laminin was deposited around Z-bodies (dot-shaped α-actinin) and integrins; from 18-to-72 h, its gradual colocalization with Z-lines (line-shaped α-actinin) and integrins increased Pearson's coefficient; this indicates that development of the BM network from the neonatal stage to adulthood is closely related to sarcomeric formation via integrin-mediated interactions.

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Mesenchymal Stem Cell-Cardiomyocyte Interactions under Defined Contact Modes on Laser-Patterned Biochips

Cited by 37 publications

References 48 publications

Design and formulation of functional pluripotent stem cell-derived cardiac microtissues

Design and formulation of functional pluripotent stem cell-derived cardiac microtissues

3D-Technology of the Formation and Maintenance of Single Dormant Microspheres from 2000 Human Somatic Cells and Their Reactivation In Vitro

Interactive relationship between basement-membrane development and sarcomerogenesis in single cardiomyocytes

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