Mesenchymal stem cell: Basic research and potential applications in cattle and buffalo

Gugjoo, Mudasir Bashir; Amarpal,; Fazili, M. R.; Shah, Riaz Ahmad; Sharma, G. Taru

doi:10.1002/jcp.27846

Cited by 27 publications

(14 citation statements)

References 170 publications

(253 reference statements)

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“…Among all domesticated species, bovine has crucial importance in the economics of the livestock industry. Many researchers have established bovine MSCs from various tissues and examined the appropriate culture conditions necessary [48]. House et al evaluated techniques to demonstrate FMDV in bovine tongue epithelium, and compared sensitivities among cell lines [49].…”

Section: Discussionmentioning

confidence: 99%

Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells

Lee

Byeon

et al. 2020

Bioscience Reports

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Mesenchymal stem cells (MSCs) possess the ability to differentiate into multiple cell lineages, and thus, confer great potential for use in regenerative medicine and biotechnology. In the present study, we attempted to isolate and characterize bovine tongue tissue epithelium-derived MSCs (boT-MSCs) and investigate the culture conditions required for long-term culturing of boT-MSCs. boT-MSCs were successfully isolated by the collagenase digestion method and their proliferative capacity was maintained for up to 20 or more passages. We observed a significant increase in the proliferation of boT-MSCs during the 20 consecutive passages under low-glucose Dulbecco’s modified Eagle’s medium culture condition among the three culture conditions. These boT-MSCs presented pluripotency markers (octamer-binding transcription factor 3/4 (Oct3/4) and sex determining region Y-box2 (Sox2)) and cell surface markers, which included CD13, CD29, CD44, CD73, CD90, CD105, CD166, and major histocompatibility complex (MHC) class I (MHC-I) but not CD11b, CD14, CD31, CD34, CD45, CD80, CD86, CD106, CD117, and MHC-II at third passage. Moreover, these boT-MSCs could differentiate into mesodermal (adipocyte, osteocyte, and chondrocyte) cell lineages. Thus, the present study suggests that the tongue of bovines could be used as a source of bovine MSCs.

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Section: Discussionmentioning

confidence: 99%

Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells

Lee

Byeon

et al. 2020

Bioscience Reports

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“…Additional in vitro studies showed that the secretome from fetal cow MSC reduced the growth of S. aureus (Cahuascanco et al, 2019) and promoted endothelial cells to form tubules, an in vitro proxy for angiogenic potential (Jervis et al, 2019). Based on currently available data, the cow MSC secretome has been proposed as a treatment for mastitis, wound healing, nerve injuries, degenerative joint diseases and other diseases of the skeletal system, as well as diabetes mellitus (Gugjoo et al, 2019;Hill et al, 2019).…”

Section: Naïve Large Animal Mscsmentioning

confidence: 99%

Translational Animal Models Provide Insight Into Mesenchymal Stromal Cell (MSC) Secretome Therapy

Harman

Marx

Walle

2021

Front. Cell Dev. Biol.

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The therapeutic potential of the mesenchymal stromal cell (MSC) secretome, consisting of all molecules secreted by MSCs, is intensively studied. MSCs can be readily isolated, expanded, and manipulated in culture, and few people argue with the ethics of their collection. Despite promising pre-clinical studies, most MSC secretome-based therapies have not been implemented in human medicine, in part because the complexity of bioactive factors secreted by MSCs is not completely understood. In addition, the MSC secretome is variable, influenced by individual donor, tissue source of origin, culture conditions, and passage. An increased understanding of the factors that make up the secretome and the ability to manipulate MSCs to consistently secrete factors of biologic importance will improve MSC therapy. To aid in this goal, we can draw from the wealth of information available on secreted factors from MSC isolated from veterinary species. These translational animal models will inspire efforts to move human MSC secretome therapy from bench to bedside.

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“…Tissues like bone marrow, adipose tissue, dental pulp, embryonic tissue, endometrium, gingiva, hair follicle, lungs, limbal epithelium, muscle, ovary, periodontal ligament, peripheral blood, periosteum, synovial fluid and membrane, umbilical cord, Wharton’s Jelly, etc. have successfully been explored as MSC sources as reviewed by Gugjoo and co-workers [ 3 , 7 , 8 , 12 , 15 ]. However, bone marrow and adipose tissue are commonly employed sources for cell harvesting due to ease of harvesting and possessing a good number of cellular concentrations [ 25 ].…”

Section: Mesenchymal Stem Cell Sources and Characterizationmentioning

confidence: 99%

“…However, limited understanding of the cellular physiology and effects of the microenvironment restricts their definitive therapeutic applications [ 8 , 9 ]. Among various stem cell types, Mesenchymal Stem Cells (MSCs) are mostly considered for preclinical and/ or clinical trials [ 10 - 12 ]. Even MSCs have been genetically modified for particular therapeutic effects (Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cell-Mediated Immuno-Modulatory and Anti- Inflammatory Mechanisms in Immune and Allergic Disorders

Gugjoo

Hussain

Amarpal

et al. 2020

IAD

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Background: Mesenchymal Stem Cells (MSCs) are present in almost all the tissues of the body and act as the backbone of the internal tissue homeostasis. Among their various characteristic features, immuno-modulatory and/ anti-inflammatory properties play an important role in therapeutics. Objective: The current topic focuses on the characterization and immuno-modulatory and/ antiinflammatory properties of MSCs. To present and discuss the current status of MSCs immunomodulatory properties. Methods: Available literature on MSCs properties and patents have been detailed, critically interpreted, and discussed based upon available literature. The main focus has been on their characteristic immunomodulatory and anti-inflammatory properties though some of the basic characterization markers have also been detailed. The databases searched for the literature include PubMed, Med Line, PubMed Central, Science Direct and a few other scientific databases. Results: MSCs are present in a very limited concentration in the tissues, and as such their culture expansion becomes imperative. MSCs immuno-modulatory and anti-inflammatory roles are achieved through direct cell-cell contact and / by the release of certain factors. Such properties are controlled by micro-environment upon which currently very limited control can be exerted. Besides, further insights in the xeno-protein free culture media as against the fetal bovine serum is required. Conclusions: MSCs have been well-isolated, cultured and characterized from numerous tissues of the body. The majority of the studies have shown MSCs as immuno-compromised with immunomodulatory and / or anti-inflammatory properties except some of the latest studies that have failed to achieve the desired results and thus, demand further research. Further research is required in the area to translate the results into clinical application.

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Mesenchymal stem cell: Basic research and potential applications in cattle and buffalo

Cited by 27 publications

References 170 publications

Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells

Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells

Translational Animal Models Provide Insight Into Mesenchymal Stromal Cell (MSC) Secretome Therapy

Mesenchymal Stem Cell-Mediated Immuno-Modulatory and Anti- Inflammatory Mechanisms in Immune and Allergic Disorders

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