Mesenchymal – Stem and non-Stem – Cells: The name of the rose

Ivanović, Zoran

doi:10.1016/j.tracli.2023.03.006

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…How are effective SCs defined? SCs have high proliferation capacity and multi-directional differentiation ability [ 176 ]. For example, cells expanded by fibroblast colony-forming units produce bone tissue in nude mice [ 177 ]; however, only a portion of these cells are polyclonal [ 176 ].…”

Section: Challenges and Prospectsmentioning

confidence: 99%

“…SCs have high proliferation capacity and multi-directional differentiation ability [ 176 ]. For example, cells expanded by fibroblast colony-forming units produce bone tissue in nude mice [ 177 ]; however, only a portion of these cells are polyclonal [ 176 ]. The minimum criteria for MSCs identified by the International Society for Cell Therapy are as follows: (1) under standard culture conditions, MSCs must adhere to plastic substrates; (2) CD105-, CD73-, and CD90-positive and CD45-, CD34-, CD24-, CD11b-, CD79a- or CD19-, and HLA-DR-negative; and (3) under in vitro standard differentiation conditions, MSCs can differentiate into osteoblasts, fat cells, and chondrocytes.…”

Section: Challenges and Prospectsmentioning

confidence: 99%

“…The minimum criteria for MSCs identified by the International Society for Cell Therapy are as follows: (1) under standard culture conditions, MSCs must adhere to plastic substrates; (2) CD105-, CD73-, and CD90-positive and CD45-, CD34-, CD24-, CD11b-, CD79a- or CD19-, and HLA-DR-negative; and (3) under in vitro standard differentiation conditions, MSCs can differentiate into osteoblasts, fat cells, and chondrocytes. Therefore, most publications refer to fibroblast-like plastic adherent cells as MSCs; however, many do not meet the basic characteristics of SCs [ 176 ]. Therefore, it is not appropriate to classify this class of MSCs as SCs.…”

Section: Challenges and Prospectsmentioning

confidence: 99%

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Progress in Research on Stem Cells in Neonatal Refractory Diseases

Huang,

He,

Zhang

et al. 2023

JPM

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With the development and progress of medical technology, the survival rate of premature and low-birth-weight infants has increased, as has the incidence of a variety of neonatal diseases, such as hypoxic–ischemic encephalopathy, intraventricular hemorrhage, bronchopulmonary dysplasia, necrotizing enterocolitis, and retinopathy of prematurity. These diseases cause severe health conditions with poor prognoses, and existing control methods are ineffective for such diseases. Stem cells are a special type of cells with self-renewal and differentiation potential, and their mechanisms mainly include anti-inflammatory and anti-apoptotic properties, reducing oxidative stress, and boosting regeneration. Their paracrine effects can affect the microenvironment in which they survive, thereby affecting the biological characteristics of other cells. Due to their unique abilities, stem cells have been used in treating various diseases. Therefore, stem cell therapy may open up the possibility of treating such neonatal diseases. This review summarizes the research progress on stem cells and exosomes derived from stem cells in neonatal refractory diseases to provide new insights for most researchers and clinicians regarding future treatments. In addition, the current challenges and perspectives in stem cell therapy are discussed.

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Section: Challenges and Prospectsmentioning

confidence: 99%

Section: Challenges and Prospectsmentioning

confidence: 99%

Section: Challenges and Prospectsmentioning

confidence: 99%

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Progress in Research on Stem Cells in Neonatal Refractory Diseases

Huang,

He,

Zhang

et al. 2023

JPM

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“…Heterogeneity poses a serious issue for further research as only a small fraction of cells in MSC populations appear to fulfill functional criteria for stem cells ( Ivanovic, 2023 ). The existence of surface antigens associated with other cell types is one of the observed aspects of MSC heterogeneity.…”

Section: Introductionmentioning

confidence: 99%

Stemness properties of SSEA-4+ subpopulation isolated from heterogenous Wharton’s jelly mesenchymal stem/stromal cells

Smolinska,

Chodkowska,

Kominek

et al. 2024

Front. Cell Dev. Biol.

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Background: High heterogeneity of mesenchymal stem/stromal cells (MSCs) due to different degrees of differentiation of cell subpopulations poses a considerable challenge in preclinical studies. The cells at a pluripotent-like stage represent a stem cell population of interest for many researchers worldwide, which is worthy of identification, isolation, and functional characterization. In the current study, we asked whether Wharton’s jelly-derived MSCs (WJ-MSCs) which express stage-specific embryonic antigen-4 (SSEA-4) can be considered as a pluripotent-like stem cell population.Methods: SSEA-4 expression in different culture conditions was compared and the efficiency of two cell separation methods were assessed: Magnetic Activated Cell Sorting (MACS) and Fluorescence Activated Cell Sorting (FACS). After isolation, SSEA-4+ cells were analyzed for the following parameters: the maintenance of the SSEA-4 antigen expression after cell sorting, stem cell-related gene expression, proliferation potential, clonogenicity, secretome profiling, and the ability to form spheres under 3D culture conditions.Results: FACS allowed for the enrichment of SSEA-4+ cell content in the population that lasted for six passages after sorting. Despite the elevated expression of stemness-related genes, SSEA-4+ cells neither differed in their proliferation and clonogenicity potential from initial and negative populations nor exhibited pluripotent differentiation repertoire. SSEA-4+ cells were observed to form smaller spheroids and exhibited increased survival under 3D conditions.Conclusion: Despite the transient expression of stemness-related genes, our findings could not fully confirm the undifferentiated pluripotent-like nature of the SSEA-4+ WJ-MSC population cultured in vitro.

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