Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers

Sfakis, Lauren; Kamaldinov, Tim; Khmaladze, Alexander; Hosseini, Zeinab F.; Nelson, Deirdre A.; Larsen, Melinda; Castracane, James

doi:10.3390/ijms19041031

Cited by 19 publications

(11 citation statements)

References 42 publications

(54 reference statements)

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“…Raman spectroscopy is often used on complex biological samples to identify chemical signatures and changes produced by alterations in the environment, such as through cell treatment [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. Inelastic Raman scattering, which occurs when there is an energy exchange between an incident photon and a molecule, results in scattered photons of different wavelengths than the original ones.…”

Section: Resultsmentioning

confidence: 99%

Telomere Length Shortening in Microglia: Implication for Accelerated Senescence and Neurocognitive Deficits in HIV

et al. 2021

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The widespread use of combination antiretroviral therapy (cART) has led to the accelerated aging of the HIV-infected population, and these patients continue to have a range of mild to moderate HIV-associated neurocognitive disorders (HAND). Infection results in altered mitochondrial function. The HIV-1 viral protein Tat significantly alters mtDNA content and enhances oxidative stress in immune cells. Microglia are the immune cells of the central nervous system (CNS) that exhibit a significant mitotic potential and are thus susceptible to telomere shortening. HIV disrupts the normal interplay between microglia and neurons, thereby inducing neurodegeneration. HIV cART contributes to the inhibition of telomerase activity and premature telomere shortening in activated peripheral blood mononuclear cells (PBMC). However, limited information is available on the effect of cART on telomere length (TL) in microglia. Although it is well established that telomere shortening induces cell senescence and contributes to the development of age-related neuro-pathologies, the effect of HIV-Tat on telomere length in human microglial cells and its potential contribution to HAND are not well understood. It is speculated that in HAND intrinsic molecular mechanisms that control energy production underlie microglia-mediated neuronal injury. TL, telomerase and mtDNA expression were quantified in microglial cells using real time PCR. Cellular energetics were measured using the Seahorse assay. The changes in mitochondrial function were examined by Raman Spectroscopy. We have also examined TL in the PBMC obtained from HIV-1 infected rapid progressors (RP) on cART and those who were cART naïve, and observed a significant decrease in telomere length in RP on cART as compared to RP’s who were cART naïve. We observed a significant decrease in telomerase activity, telomere length and mitochondrial function, and an increase in oxidative stress in human microglial cells treated with HIV Tat. Neurocognitive impairment in HIV disease may in part be due to accelerated neuro-pathogenesis in microglial cells, which is attributable to increased oxidative stress and mitochondrial dysfunction.

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Section: Resultsmentioning

confidence: 99%

Telomere Length Shortening in Microglia: Implication for Accelerated Senescence and Neurocognitive Deficits in HIV

et al. 2021

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“…The use of 3D systems allows epithelial-mesenchymal interaction and differentiation induction driven by paracrine factors synthesized and secreted by tissue specific stromal cells immersed in the biomaterial [39,40]. The use of these systems demonstrated to be effective in inducing ex vivo differentiation into different tissue types [18,41,42], although we only found a partial effect.…”

Section: Discussionmentioning

confidence: 68%

Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells

et al. 2021

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Several models of bioartificial human urothelial mucosa (UM) have been described recently. In this study, we generated novel tubularized UM substitutes using alternative sources of cells. Nanostructured fibrin–agarose biomaterials containing fibroblasts isolated from the human ureter were used as stroma substitutes. Then, human Wharton jelly mesenchymal stromal cells (HWJSC) were used to generate an epithelial-like layer on top. Three differentiation media were used for 7 and 14 days. Results showed that the biofabrication methods used here succeeded in generating a tubular structure consisting of a stromal substitute with a stratified epithelial-like layer on top, especially using a medium containing epithelial growth and differentiation factors (EM), although differentiation was not complete. At the functional level, UM substitutes were able to synthesize collagen fibers, proteoglycans and glycosaminoglycans, although the levels of control UM were not reached ex vivo. Epithelial differentiation was partially achieved, especially with EM after 14 days of development, with expression of keratins 7, 8, and 13 and pancytokeratin, desmoplakin, tight-junction protein-1, and uroplakin 2, although at lower levels than controls. These results confirm the partial urothelial differentiative potential of HWJSC and suggest that the biofabrication methods explored here were able to generate a potential substitute of the human UM for future clinical use.

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“…Its special attributes include good biocompatibility, excellent mechanical properties, regulatable biodegradability, and electrospinnability. However, due to the poor hydrophilicity of PLGA, its applications in regenerative medicine have been limited 8,9 …”

Section: Introductionmentioning

confidence: 99%

Fabrication of multilayered electrospun poly(lactic‐co‐glycolic acid)/polyvinyl pyrrolidone + poly(ethylene oxide) scaffolds and biocompatibility evaluation

Jiao

Liu

et al. 2020

J Biomedical Materials Res

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Poly(lactic‐co‐glycolic acid)/polyvinyl pyrrolidone + poly(ethylene oxide) [PLGA/(PVP + PEO)] scaffolds with different polymer concentrations were fabricated using multilayered electrospinning, and their physicochemical properties and biocompatibility were examined to screen for scaffolds with excellent performance in tissue engineering (TE). PLGA solution (15% w/v) was used as the bottom solution, and a mixed solution of 12% w/v PVP + PEO was applied as the surface layer solution. The mass ratios of PVP vs. PEO in each 10 ml surface layer mixed solution were 1.08 g: 0.12 g; 0.96 g: 0.24 g; and 0.84 g: 0.36 g. Compared to the conventional electrospinning method used to fabricate the pure PVP + PEO (0.96 g: 0.24 g, Group A) scaffold and pure PLGA (Group E) scaffold, the multilayer electrospinning technique of alternating sprays of the bottom layer solution and the surface layer solution was adopted to fabricate multilayer nanofiber scaffolds, including PLGA/(PVP + PEO) (1.08 g: 0.12 g, Group B), PLGA/(PVP + PEO) (0.96 g: 0.24 g, Group C), and PLGA/(PVP + PEO) (0.84 g: 0.36 g, Group D). The morphology and characteristics of the five scaffolds were analyzed, and the biocompatibilities of the cell‐scaffold composites were assessed through methods including Cell Counting Kit‐8 (CCK8) analysis, 4′,6‐diamidino‐2‐phenylindole (DAPI) staining, and scanning electron microscopy. Therefore, with a PVP‐to‐PEO mass ratio of 0.96 g: 0.24 g, an optimal multilayer nanofiber scaffold was fabricated by the multilayer electrospinning technique. The excellent biocompatibility and mechanical properties of the scaffold were confirmed by in vitro experiments, which demonstrated the scaffold's promising application potential in the field of TE.

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Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers

Cited by 19 publications

References 42 publications

Telomere Length Shortening in Microglia: Implication for Accelerated Senescence and Neurocognitive Deficits in HIV

Telomere Length Shortening in Microglia: Implication for Accelerated Senescence and Neurocognitive Deficits in HIV

Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells

Fabrication of multilayered electrospun poly(lactic‐co‐glycolic acid)/polyvinyl pyrrolidone + poly(ethylene oxide) scaffolds and biocompatibility evaluation

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