Mesencephalic dopaminergic neurons in primary cultures express functional neurotensin receptors

Brouard, Aline; Pélaprat, Didier; Dana, C; Vial, Micheline; Lhiaubet, Anne-Marie; Rostène, William

doi:10.1523/jneurosci.12-04-01409.1992

Cited by 49 publications

(32 citation statements)

References 32 publications

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“…Furthermore, high-affinity NT receptors in this area have been shown to be selectively associated with dopaminergic (DA) neurons in both rodent and primate brain (Palacios and Kuhar, 1982;Sadoul et al, 1984;Quirion et al, 1987;Szigethy and Beaudet, 1989). In keeping with these results, rat embryonic DA neurons were found to be enriched with functional NT receptors in primary cell cultures from the rat midbrain (Chabry et al, 1990;Dana et al, 1991;Brouard et al, 1992). Pure glial cultures were taken from the neocortex of neonatal animals, because this region was reported to express among the highest levels of low-affinity NT receptors in the adult rat brain (Chalon et al, 1996;Mazella et al, 1996).…”

Section: Abstract: Confocal Microscopy; Immunohistochemistry; Tyrosimentioning

confidence: 54%

Differential Binding Profile and Internalization Process of Neurotensin via Neuronal and Glial Receptors

et al. 1997

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Two G-protein-coupled receptors for the tridecapeptide neurotensin (NT) have been identified and cloned in mammalian brain: a high-affinity (K d ϭ 0.3 nM) receptor, sensitive to the antagonist SR 48692 but insensitive to levocabastine, and a lower-affinity (K d ϭ 2-4 nM) receptor, sensitive to levocabastine but with poor affinity for SR 48692. Although there is good evidence that the high-affinity site is predominantly expressed in neurons, little is known of the cellular localization of the low-affinity receptor. In the present study, we identify by confocal microscopy selective levocabastine-sensitive, SR 48692-resistant binding of a fluorescent derivative of NT (fluo-NT) to a subpopulation of glial fibrillary acidic protein-immunoreactive glial cells grown in culture from the midbrain and cerebral cortex of embryonic and neonatal rats, respectively. We also demonstrate, by combining fluo-NT detection with tyrosine hydroxylase immunofluorescence, that these glial binding sites are differentially regulated from the SR 48692-sensitive NT receptor expressed in the same cultures by mesencephalic dopamine neurons. Whereas the latter undergoes rapid ligandinduced internalization followed by centripetal mobilization of ligand-receptor complexes from processes to perikarya and from perikaryal periphery to cell center, the former induces the formation of cell-surface clusters that fail to internalize. It is concluded that NT may exert its effects on both neurons and astrocytes in the CNS. Whereas NT neural signaling is exerted through high-affinity receptors and may be partly effected through internalization of receptor-ligand complexes, glial signaling is exerted through low-affinity NT receptors and appears to be transduced exclusively at the level of the plasma membrane.

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Section: Abstract: Confocal Microscopy; Immunohistochemistry; Tyrosimentioning

confidence: 54%

Differential Binding Profile and Internalization Process of Neurotensin via Neuronal and Glial Receptors

et al. 1997

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“…In connection with such findings, previous studies combining in vitro autoradiography of NT binding sites and TH immunocytochemistry on tissue sections (Szigethy and Beaudet, 1989) or on primary rat mesencephalic cells in culture (Brouard et al, 1992) reported that about 20% of DAergic neurons of the mesencephalon did not seem to express the NTR. Finally, NTR but not TH mRNA-expressing cells were detected in the lateral part of the supramammillary nucleus, on the border of the ventral tegmental area.…”

Section: Discussionmentioning

confidence: 78%

Differential expression of neurotensin receptor mRNA in the dopaminergic cell groups of the rat diencephalon and mesencephalon

Nicot

Rostène

Bérod

1995

J of Neuroscience Research

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Several lines of evidence support interactions between neurotensin (NT) and dopaminergic (DAergic) neurons in the brain. In order to obtain further knowledge about the anatomical substrate for such interactions, the distribution of cells expressing the cloned neurotensin receptor (NTR) mRNA was examined in relation to tyrosine hydroxylase (TH) mRNA-expressing cells within different subnuclei of the diencephalon and ventral mesencephalon of the male rat. In situ hybridization was performed on consecutive sections labeled with 33P-labeled oligonucleotide probes. In the hypothalamus, NTR mRNA signals were mostly found in the suprachiasmatic, dorsomedial, dorsal premammillary, and supramammillary nuclei. On the other hand, DAergic cells of the periventricular nucleus of the hypothalamus and dorsal aspect of the arcuate nucleus, revealed by TH in situ hybridization, did not exhibit NTR mRNA even though dense NT binding sites have been previously described in this nuclei. In the zona incerta, TH mRNA-containing cells were concentrated in the medial part, with little overlap with NTR mRNA-expressing cells located mainly in its mediolateral extent. In contrast, the distribution of both markers was superimposable within the different subdivisions of the ventral tegmental area and substantia nigra, as previously suggested, but also in the retrorubral field. These anatomical data further support the NT-dopamine interactions on both mesocorticolimbic and nigrostriatal DAergic systems. Moreover, the results suggest that diencephalic DAergic neurons do not synthesize the cloned NTR mRNA or express it at considerably lower levels than DAergic mesencephalic cells.

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“…The NTSR1 gene encodes a high-affinity neurotensin receptor, NTSR1. In the central nervous system, NT and NTSR1 are highly expressed in dopaminergic neurons (Brouard et al, 1992;Studler et al, 1988), which allows functional interactions between the NT and the DA systems at the cellular level (Binder et al, 2001). For example, animal studies suggested that NT enhances DA neuron firing (Jomphe et al, 2006;St-Gelais et al, 2004) and extracellular release of DA (Petkova-Kirova et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

The NTSR1 gene modulates the association between hippocampal structure and working memory performance

Jin¹,

Lei²,

Wang³

et al. 2013

NeuroImage

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The genetic and neural basis of working memory (WM) has been extensively studied. Many dopamine (DA) related genes, including the NTSR1 gene (a DA modulator gene), have been reported to be associated with WM performance. The NTSR1 protein is predominantly expressed in the cerebral cortex and the hippocampus, the latter of which is closely involved in WM processing based on both lesion and fMRI studies. Thus far, however, no study has examined the joint effects of NTSR1 gene polymorphism and hippocampal morphology on WM performance. Participants of the current study were 330 healthy Chinese college students. WM performance was measured with a 2-back WM paradigm. Structural MRI data were acquired and then analyzed using an automated procedure with atlas-based FreeSurfer segmentation software (v 4.5.0) package. Linear regression analyses were conducted with a NTSR1 C/T polymorphism which was previously reported to be associated with WM (rs4334545), hippocampal volume, and their interaction as predictors of WM performance, with gender and intracranial volume (ICV) as covariates. Results showed a significant interaction between NTSR1 genotype and hippocampal volume (p b .05 for both the left and right hippocampi). Further analysis showed that the correlation between hippocampal volume and WM scores was significant for carriers of the NTSR1 T-allele (p b .05 for both hippocampi), but not for CC homozygotes. These results indicate that the association between hippocampal structure and WM performance was modulated by variation in the NTSR1 gene, and suggest that further studies of brain-behavior associations should take genetic background information into account.

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Mesencephalic dopaminergic neurons in primary cultures express functional neurotensin receptors

Cited by 49 publications

References 32 publications

Differential Binding Profile and Internalization Process of Neurotensin via Neuronal and Glial Receptors

Differential Binding Profile and Internalization Process of Neurotensin via Neuronal and Glial Receptors

Differential expression of neurotensin receptor mRNA in the dopaminergic cell groups of the rat diencephalon and mesencephalon

The NTSR1 gene modulates the association between hippocampal structure and working memory performance

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