Mesectodermal cell fate analysis in Drosophila midline mutants

Sonnenfeld, Margaret; Jacobs, J. Roger

doi:10.1016/0925-4773(94)90033-7

Cited by 64 publications

(38 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, we demonstrate that expression of Pvfs alone is not sufficient for this process (or even required for migration along the dorsal side of the VNC) and that the integrity of the VNC is also fundamental. We cannot exclude the loss of other chemoattractants in slit and robo1,2 mutants, but the failure of the VNC to separate from the epidermis seems a more likely explanation: first, unlike sim mutants, slit mutants maintain expression of many midline genes (Sonnenfeld and Jacobs, 1994); second, the sim and slit haemocyte phenotypes are very similar, suggesting that those genes that are lost play minor roles, if any, in the regulation of this process.…”

Section: Interdependence Of Vnc Development and Haemocyte Migrationmentioning

confidence: 93%

See 1 more Smart Citation

Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos

Evans

Skaer

et al. 2010

Development

View full text Add to dashboard Cite

SUMMARYDuring embryonic development, Drosophila macrophages (haemocytes) undergo a series of stereotypical migrations to disperse throughout the embryo. One major migratory route is along the ventral nerve cord (VNC), where haemocytes are required for the correct development of this tissue. We show, for the first time, that a reciprocal relationship exists between haemocytes and the VNC and that defects in nerve cord development prevent haemocyte migration along this structure. Using live imaging, we demonstrate that the axonal guidance cue Slit and its receptor Robo are both required for haemocyte migration, but signalling is not autonomously required in haemocytes. We show that the failure of haemocyte migration along the VNC in slit mutants is not due to a lack of chemotactic signals within this structure, but rather to a failure in its detachment from the overlying epithelium, creating a physical barrier to haemocyte migration. This block of haemocyte migration in turn disrupts the formation of the dorsoventral channels within the VNC, further highlighting the importance of haemocyte migration for correct neural development. This study illustrates the important role played by the three-dimensional environment in directing cell migration in vivo and reveals an intriguing interplay between the developing nervous system and the blood cells within the fly, demonstrating that their development is both closely coupled and interdependent.

show abstract

Section: Interdependence Of Vnc Development and Haemocyte Migrationmentioning

confidence: 93%

“…Development 137 (10) in these mutants (Sonnenfeld and Jacobs, 1994), a subset of Pvf2-expressing cells was found at the ventral surface of the VNC (Fig. 6I,J).…”

Section: Research Articlementioning

confidence: 95%

Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos

Evans

Skaer

et al. 2010

Development

View full text Add to dashboard Cite

show abstract

“…7) might not indicate a direct dendrogenic role for Slit. Even before the normal onset of aCC dendrogenesis the CNS midline collapses in these mutant embryos Rothberg et al, 1990;Sonnenfeld and Jacobs, 1994). At the time dendrogenesis would normally occur, the CNS lacks a neuropil (Fig.…”

Section: Potential Role Of Slit At Neuropilmentioning

confidence: 94%

Slit and Robo control the development of dendrites inDrosophilaCNS

et al. 2007

View full text Add to dashboard Cite

The molecular mechanisms that generate dendrites in the CNS are poorly understood. The diffusible signal molecule Slit and the neuronally expressed receptor Robo mediate growth cone collapse in vivo. However, in cultured neurons, these molecules promote dendritic development. Here we examine the aCC motoneuron, one of the first CNS neurons to generate dendrites in Drosophila. Slit displays a dynamic concentration topography that prefigures aCC dendrogenesis. Genetic deletion of Slit leads to complete loss of aCC dendrites. Robo is cell-autonomously required in aCC motoneurons to develop dendrites. Our results demonstrate that Slit and Robo control the development of dendrites in the embryonic CNS.

show abstract

“…This phenotype was originally thought to represent a defect in the specification of midline cell fate (Rothberg et al, 1988;Rothberg et al, 1990). Although a ventral displacement of midline cells results in the fusion of the longitudinal connectives shortly after commissure formation, the primary axonal defect in slit loss-offunction mutants is the inability of commissural axons to leave the midline (Sonnenfeld and Jacobs, 1994). In retrospect, this is precisely the type of defect expected for a gene that purportedly functions as a midline repellent.…”

Section: Drosophila Ventral Nerve Cordmentioning

confidence: 99%

Axon guidance at the midline choice point

Kaprielian

Runko

Imondi

2001

Developmental Dynamics

145

108

View full text Add to dashboard Cite

The central nervous system (CNS) of higher organisms is bilaterally-symmetric. The transfer of information between the two sides of the nervous system occurs through commissures formed by neurons that project axons across the midline to the contralateral side of the CNS. Interestingly, these axons cross the midline only once. Other neurons extend axons that never cross the midline; they project exclusively on their own (ipsilateral) side of the CNS. Thus, the midline is an important choice point for several classes of pathfinding axons. Recent studies demonstrate that specialized midline cells play critical roles in regulating the guidance of both crossing and non-crossing axons at the ventral midline of the developing vertebrate spinal cord and the Drosophila ventral nerve cord. For example, these cells secrete attractive cues that guide commissural axons over long distances to the midline of the CNS. Furthermore, shortrange interactions between guidance cues present on the surfaces of midline cells, and their receptors expressed on the surfaces of pathfinding axons, allow commissural axons to cross the midline only once and prevent ipsilaterally-projecting axons from entering the midline. Remarkably, the molecular composition of commissural axon surfaces is dynamically-altered as they cross the midline. Consequently, commissural axons become responsive to repulsive midline guidance cues that they had previously ignored on the ipsilateral side of the midline. Concomitantly, commissural axons lose responsiveness to attractive guidance cues that had initially attracted them to the midline. Thus, these exquisitely regulated guidance systems prevent commissural axons from lingering within the confines of the midline and allow them to pioneer an appropriate pathway on the contralateral side of the CNS. Many aspects of midline guidance are controlled by mechanistically and evolutionarily-conserved ligand-receptor systems. Strikingly, recent studies demonstrate that these receptors are modular; the ectodomains determine ligand recognition and the cytoplasmic domains specify the response of an axon to a given guidance cue. Despite rapid and dramatic progress in elucidating the molecular mechanisms that control midline guidance, many questions remain.

show abstract

Mesectodermal cell fate analysis in Drosophila midline mutants

Cited by 64 publications

References 28 publications

Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos

Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos

Slit and Robo control the development of dendrites inDrosophilaCNS

Axon guidance at the midline choice point

Contact Info

Product

Resources

About