Meropenem as an Alternative Antibiotic Agent for Suppression of Agrobacterium in Genetic Transformation of Orchid

Cao, Ying; Niimi, Yoshiyuki; Hu, Shanglian

doi:10.1016/s1671-2927(06)60133-8

Cited by 9 publications

(5 citation statements)

References 29 publications

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“…Meropenem was practically well tolerated except for the concentration of 20 mg/l in slowly growing line 28. Similar effects of meropenem were described on somatic embryogenesis of orchids (Cao et al 2006;Ogava, Mii 2007). Kanamycin was tolerated by the embryogenic tissue of all lines only in the concentration of 10 mg/l.…”

Section: Discussionsupporting

confidence: 67%

Tolerance of Norway spruce (Picea abies [L.] Karst.) embryogenic tissue to penicillin, carbapenem and aminoglycoside antibiotics

Malá¹,

Pavingerová²,

Cvrčková³

et al. 2009

J. For. Sci.

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Somatic embryogenesis is conveniently utilized for the preparation of Norway spruce (Picea abies [L.] Karst.) transgenic clones by means of Agrobacterium. The establishment of successful transformation protocol requires to determine the tolerance of growing embryogenic tissue to antibiotics in culture and selective media. In 5 Norway spruce lines (genotypes) differences in the tolerance of embryogenic tissues to penicillin antibiotics (amoxicillin, carbenicillin, and ticarcillin), carbapenem antibiotic (meropenem) used for the Agrobacterium growth prevention, and aminoglycoside antibiotic (kanamycin) used in selective media were determined. Of the penicillin derivatives, amoxicillin was optimally tolerated in all lines and, in addition, its highest concentration accelerated growth in more rapidly growing lines. Ticarcillin was similarly tolerated but no growth acceleration was observed in any line. As regards carbenicillin, only the lowest concentration was observed to be well tolerated by all lines whereas all concentrations of meropenem were well tolerated in all lines except for slowly growing line 28, the growth of which was retarded by the concentration of 20 mg/l. The aminoglycoside antibiotic kanamycin was well tolerated by the embryonic tissue of all lines in the concentration of 10 mg/l and less in the concentration of 25 mg/l. The concentrations of 50 mg/l and 100 mg/l appeared as intolerable in all lines. Toxicity of kanamycin manifested at first in the browning and later in the growth cessation of embryogenic tissue.

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Section: Discussionsupporting

confidence: 67%

Tolerance of Norway spruce (Picea abies [L.] Karst.) embryogenic tissue to penicillin, carbapenem and aminoglycoside antibiotics

Malá¹,

Pavingerová²,

Cvrčková³

et al. 2009

J. For. Sci.

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“…To begin our characterization, we first assessed the susceptibility of A. tumefaciens to different concentrations of ampicillin near the MIC reported for A. tumefaciens on solid and liquid media ( 70 ). We found that cells grown in LB medium with 25 or 50 μg/mL ampicillin (AMP 25 or AMP 50, respectively) for 24 h displayed slow growth in liquid medium in comparison to cells grown in LB medium without ampicillin (LB No AMP) ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Induction of AmpC-Mediated β-Lactam Resistance Requires a Single Lytic Transglycosylase in Agrobacterium tumefaciens

Figueroa-Cuilan

Howell

Richards

et al. 2022

Appl Environ Microbiol

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“…Enhanced disease resistance [16] Dendrobium Phalaenopsis Agrobacterium-mediated transformation of gusA, nptII, hptII marker genes Successful insertion of genes in transgenic orchid [17] Phalaenopsis sp. Phalaenopsis sp.…”

Section: A Tumefaciens-mediated Transformation Of the Orchidmentioning

confidence: 99%

Advances in Orchid Biology: Biotechnological Achievements, Translational Success, and Commercial Outcomes

Tiwari,

Sharma,

Bose

et al. 2024

Horticulturae

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Orchids constitute the largest and most diverse group of flowering plants and are classified in the family Orchidaceae. Exhibiting significance as the most exotic and ubiquitous flowering plant, the cultivation of orchids on a commercial level is gaining momentum worldwide. In addition to its ornamental and aesthetic value, the orchid industry has successfully generated employment for people in developing countries. Recent advances in biotechnological interventions in orchids have substantially contributed to the development of exotic varieties with novel traits, not to forget the inputs of traditional plant breeding methods and tissue culture approaches. In addition, the scientific developments in orchid biology have remarkably bridged the knowledge gaps in areas of orchid classification, phytochemistry, and cultivation strategies. This has facilitated the commercialization of novel varieties, opening new avenues in the orchid industry, and their global marketing as cut flowers and artificially propagated plants. Orchids constitute the first floriculture crops that revolutionized the orchid industry; however, they also hold several challenges in the natural propagation and conservation of several species that are on the verge of extinction. International organizations like CITES have come forward to address challenges associated with illegal global trade and indiscriminate use of orchid varieties, aiming for conservation and legal commercial goals. This thematic review is one-of-a-kind in providing comprehensive insights into the emerging momentum of orchid biology and how its globalization projects to considerably impact the orchid industry in the coming times. However, it is imperative to understand the challenges in the cultivation and conservation of orchid varieties and ensure legislative guidelines both on domestic and global levels to ensure a multipronged approach to the conservation and commercialization of orchids.

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Meropenem as an Alternative Antibiotic Agent for Suppression of Agrobacterium in Genetic Transformation of Orchid

Cited by 9 publications

References 29 publications

Tolerance of Norway spruce (Picea abies [L.] Karst.) embryogenic tissue to penicillin, carbapenem and aminoglycoside antibiotics

Tolerance of Norway spruce (Picea abies [L.] Karst.) embryogenic tissue to penicillin, carbapenem and aminoglycoside antibiotics

Induction of AmpC-Mediated β-Lactam Resistance Requires a Single Lytic Transglycosylase in Agrobacterium tumefaciens

Advances in Orchid Biology: Biotechnological Achievements, Translational Success, and Commercial Outcomes

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