Merocyanine 540 mediated photolysis of normal bone marrow, committed hemopoietic progenitors and neoplastic cells. Implications for bone marrow purging

Lydaki, E.; Dimitriou, Helen; Papazoglou, Theodore G.; Liu, Wen Qing; Kalmanti, Maria

doi:10.1016/s0145-2126(97)00023-4

Cited by 11 publications

(9 citation statements)

References 34 publications

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“…Autologous HSCT (Auto-HSCT) enjoys several advantages over allogeneic HSCT (Allo-HSCT): easy transplantation of grafts, without the restriction of age and the need for an HLA-matched donor, the absence of graft-versus-host disease (GVHD) and a small percentage of life-threatening infections. [1][2][3][4] It has been proven that auto-HSCT is a promising approach for leukemia therapy. However, it still has its limitation of high relapse rate, as compared with Allo-HSCT and the limitation is ascribed to the risk of reinfusing residual leukemic cells at the time of harvest.…”

Section: Introductionmentioning

confidence: 99%

Purging of murine erythroblastic leukemia by ZnPcS2P2-based-photodynamic therapy

Huang

Chen

et al. 2005

Bone Marrow Transplant

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A key point for successful transplantation of autologous hematopoietic stem cells in the treatment of leukemia is the purging technique, of which photodynamic therapy (PDT) proved effective and promising. The aim of this study was to evaluate the purging effect of a novel amphipathic photosensitizer, di-sulfo-di-phthalimidomethyl phthalolcyanine zinc (ZnPcS 2 P 2 )-based PDT (ZnPcS 2 P 2 -PDT) on murine erythroblastic leukemic EL9611 cells. Bone marrow cells (BMC), harvested from normal BALB/c mice, were contaminated with variable EL9611 cells. Cell suspensions were incubated with 4 lg/ml ZnPcS 2 P 2 for 5 h and then exposed to 2.1 J/cm 2 irradiation by a semiconductor laser 670 nm. Lethally irradiated recipient BALB/c mice (7 Gy) received syngeneic bone marrow transplantation with purged or nonpurged cell mixtures of 10 7 BMC contaminated with variable numbers (10 2 -10 5 ) of EL9611 cells. All of the irradiated controls died due to sepsis. All of the mice injected with nonpurged cell mixtures developed leukemia and died, whereas the mice transplanted with ZnPcS 2 P 2 -PDT-treated mixtures had a longer survival time, and the fewer leukemic cells there were in the cell mixtures, the higher the leukemia-free survival rate. We conclude that ZnPcS 2 P 2 -PDT could purge leukemic cells from bone marrow autografts but could retain sufficient progenitor cells for the hematopoietic activity.

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Section: Introductionmentioning

confidence: 99%

Purging of murine erythroblastic leukemia by ZnPcS2P2-based-photodynamic therapy

Huang

Chen

et al. 2005

Bone Marrow Transplant

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“…In summary, this experimental study draws the following conclusions: (1) dark incubation with ALA can accumulate some PpIX in HL60 cell and PBMC can synthesize significantly less PpIX and (2) when HL60 cells (1 × 10 5 /mL) were incubated with ALA (1 mmol/L) in dark for 4 h, and then irradiated with 410 nm (4 mW/cm 2 , 60 min) for 14.4 J/cm 2 , the maximum photodamage to membrane and mitochondrial function of HL60 cell would result. As to the normal granulocytes, such response was not detected.…”

Section: Discussionmentioning

confidence: 61%

“…7). The concentration of the dots in four quadrants shows four distinct cell populations: (1) fraction of cells whose membrane was photodamaged, these cells have low FITC and high PI signal; (2) fraction of secondary necrotic cells which have high FITC and high PI signal; (3) the viable cells which were not damaged during collection and have low FITC and low PI signal; (4) the apoptotic cells which have high FITC and low PI signal. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Several photosensitizers in PDT have so far been applied to eradicate residual tumor cells in autografts and to reduce contamination of tumor cells in hemopathy (1–3). However, all the exogenous photosensitizers have a limited selective accumulation in tumor cells, and a limited selective photodynamic destruction of the tumor cells thus inevitably ensues.…”

Section: Introductionmentioning

confidence: 99%

“…Because many researchers applied PDT to eradicate residual tumor cells in autografts (purging) and to reduce contamination of tumor cells (1–3), they have got exciting results: a higher uptake of photosensitizer in malignant blood cells and subsequent light exposure selectively kills these cells with minimal toxicity to progenitor cells or stem cells. However, the effect of PDT may be modulated by the leukemia cell type; the photosensitizer's type, dose, dark incubation time; the light waveband, dosage.…”

Section: Introductionmentioning

confidence: 99%

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5‐Aminolevulinic Acid‐Based Photodynamic Therapy in Leukemia Cell HL60^¶

Zhang¹,

Zhang²

2004

Photochem & Photobiology

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A study to explore the optimal experimental parameters and the photosensitization of 5‐aminolevulinic acid (ALA)‐based photodynamic therapy (PDT) in promyelocytic leukemia cell HL60 has been conducted, in which HL60 cells and their control groups, peripheral blood mononuclear cell (PBMC), first are incubated with different concentrations of ALA in dark for different periods of time and then followed by irradiating with different wavebands for different fluences. Fluorescence microscope and spectrofluorometer have been used to detect the fluorescence of protoporphyrin IX (PpIX) endogenously produced by ALA. The response of the cells to ALA‐PDT was evaluated by the 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2‐5‐diphenyl‐2H‐tetrazolium bromide (MTT) assay (interval between irradiation and the MTT assay is 24 h) and by flow cytometry (the length of time between irradiation and the flow assay is 30 min). MTT results will reflect the relative number of metabolically active mitochondria in the population. Propidium iodide uptake in flow cytometry will test for membrane damage. The results of parameter experiments were obtained: 1 × 105/mL HL60 cell was first incubated with 1 mmol/L ALA in dark for 4 h and the maximum fluorescence of PpIX level appeared; then irradiated with 410 nm (4 mW/cm2) for 14.4 J/cm2 and maximum photodamage to membrane and mitochondrial function of HL60 cell resulted. With the normal granulocytes, such response was not detected. Therefore a hypothetical idea can be brought forward that ALA‐based PDT can be used for inactivation of leukemia cell HL60 and these optimal parameters may be useful for clinical application.

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Femtosecond laser induced photodynamic therapy on 5-ALA treated SKMEL-30 cells: An efficient theranostic strategy to combat melanoma

Kars

Kara

Gündoğdu

et al. 2014

Biomedicine & Pharmacotherapy

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Merocyanine 540 mediated photolysis of normal bone marrow, committed hemopoietic progenitors and neoplastic cells. Implications for bone marrow purging

Cited by 11 publications

References 34 publications

Purging of murine erythroblastic leukemia by ZnPcS2P2-based-photodynamic therapy

Purging of murine erythroblastic leukemia by ZnPcS2P2-based-photodynamic therapy

5‐Aminolevulinic Acid‐Based Photodynamic Therapy in Leukemia Cell HL60^¶

Femtosecond laser induced photodynamic therapy on 5-ALA treated SKMEL-30 cells: An efficient theranostic strategy to combat melanoma

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Merocyanine 540 mediated photolysis of normal bone marrow, committed hemopoietic progenitors and neoplastic cells. Implications for bone marrow purging

Cited by 11 publications

References 34 publications

Purging of murine erythroblastic leukemia by ZnPcS2P2-based-photodynamic therapy

Purging of murine erythroblastic leukemia by ZnPcS2P2-based-photodynamic therapy

5‐Aminolevulinic Acid‐Based Photodynamic Therapy in Leukemia Cell HL60¶

Femtosecond laser induced photodynamic therapy on 5-ALA treated SKMEL-30 cells: An efficient theranostic strategy to combat melanoma

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Product

Resources

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5‐Aminolevulinic Acid‐Based Photodynamic Therapy in Leukemia Cell HL60^¶