Meristem identity gene expression during curd proliferation and flower initiation in Brassica oleracea

Duclos, Denise V.; Björkman, Thomas

doi:10.1093/jxb/erm327

Cited by 44 publications

(39 citation statements)

References 53 publications

(54 reference statements)

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“…As the distinctive commodity organ of cauliflower, curds showed abundant morphological variations, which have been intensively studied at molecular genetic levels [1]. For example, the specific curd phenotype is revealed to be governed by BoCAL gene [2,3], BoAP1 gene determines the divergence between cauliflower curd and broccoli head phenotypes [4,5], while the Or Gene encoding a plastid associated protein results in an orange curd that is rich in β-carotene [6]. Recently, a genome wide association study based on 174 randomly selected cauliflower gene bank accessions identified a total of 24 significant associations for curd-related traits [7].…”

Section: Introductionmentioning

confidence: 99%

Identification of QTLs associated with curd architecture in cauliflower

Zhao

Sheng

et al. 2020

BMC Plant Biol

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Background: Curd architecture is one of the most important characters determining the curd morphology of cauliflower. However, the genetic mechanism dissection of this complex trait at molecular level is lacking. Genes/ QTLs responsible for the morphological differences between present-day loose-curd and compact-curd cauliflower haven't been well revealed. Results: Herein, by using a common compact-curd parent and two loose-curd parents, we developed two double haploid (DH) populations including 122 and 79 lines, respectively. For each population, we decomposed the curd architecture concept into four parameters (basal diameter, stalk length, stalk angle and curd solidity), and collected corresponding phenotypic data for each parameter across two environments. The Kosambi function and composite interval mapping algorithm were conducted to construct the linkage map and analyze the QTLs associated with curd architecture parameters. A total of 20 QTLs were detected with the minimum likelihood of odd (LOD) values ranging from 2.61 to 8.38 and the percentage of the phenotypic variance explained by each QTL (PVE) varying between 7.69 and 25.10%. Of these, two QTLs controlling stalk length (qSL.C6-1, qSL.C6-2) and two QTLs controlling curd solidity (qCS.C6-1 and qCS.C6-2) were steadily expressed in both environments. Further, qSL.C6-1, qSL.C6-2, qCS.C6-1 and qCS.C6-4 fell into the same chromosomal region of the reference genome, indicating that these loci are involved in pleiotropic effects or are tightly linked. Conclusion: The current study identified a series of QTLs associated with curd architecture parameters, which might contribute essentially to the formation of present-day loose-curd cauliflower that is widely cultivated in China. These results may pave the way for intensive deciphering the molecular mechanisms of curd development and for marker-assisted selection of curd morphology in cauliflower breeding.

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Section: Introductionmentioning

confidence: 99%

Identification of QTLs associated with curd architecture in cauliflower

Zhao

Sheng

et al. 2020

BMC Plant Biol

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“…Meristem identity genes have been suggested as possible candidates involved in development arrest. However, Duclos & Björkman (2008) concluded from an expression study carried out on broccoli and cauliflower that development arrest is not caused by suppression of B. oleracea meristem identity homologues such as Bo‐AP and Bo‐LFY .…”

Section: Discussionmentioning

confidence: 99%

“…1996). However, a study on the expression of meristem identity genes including Bo‐LFY and Bo‐AP showed that arrest in development at the enlargement of inflorescence meristems (cauliflower) or flower buds (broccoli) was not controlled by homologues specifying these steps in Arabidopsis (Duclos & Björkman 2008).…”

Section: Introductionmentioning

confidence: 99%

Prediction of flowering time in Brassica oleracea using a quantitative trait loci‐based phenology model

Uptmoor

Schrag

et al. 2011

Plant Biology

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Uniformly developing plants with a predictable time to harvest or flowering under unfavourable climate conditions are a major breeding goal in crop species. The main flowering regulators and their response to environmental signals have been identified in Arabidopsis thaliana and homologues of flowering genes have been mapped in many crop species. However, it remains unclear which genes determine within and across genotype flowering time variability in Brassica oleracea and how genetic flowering time regulation is influenced by environmental factors. The goal of this study is model-based prediction of flowering time in a B. oleracea DH-line population using genotype-specific and quantitative trait loci (QTL) model input parameters. A QTL-based phenology model accounting for genotypic differences in temperature responses during vernalisation and non-temperature-sensitive durations from floral transition to flowering was evaluated in two field trials. The model was parameterised using original genotype-specific model input parameters and QTL effects. The genotype-specific model parameterisation showed accurate predictability of flowering time if floral induction was promoted by low temperature (R(2) = 0.81); unfavourably high temperatures reduced predictability (R(2) = 0.65). Replacing original model input parameters by QTL effects reduced the capability of the model to describe across-genotype variability (R(2) = 0.59 and 0.50). Flowering time was highly correlated with a model parameter accounting for vernalisation effects. Within-genotype variability was significantly correlated with the same parameter if temperature during the inductive phase was high. We conclude that flowering time variability across genotypes was largely due to differences in vernalisation response, although it has been shown elsewhere that the candidate FLOWERING LOCUS C (FLC) did not co-segregate with flowering time in the same population. FLC independent vernalisation pathways have been described for several species, but not yet for B. oleracea.

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“…QTLs conferring days to curd initiation (DCI) are influenced by ambient temperature, as exemplified by only 2 QTLs were consistently detected in a doubled haploid (DH) population and a commercial diversity panel of cauliflower, respectively, at several temperature regimes 22 , 26 . BoVRN2 and BoFLC2 were not related to temperature-regulated curd induction, and suppression of BoCAL , BoAP1-a , and BoLFY or failure to suppress BoTFL1 could maintain arrest of the inflorescence meristem but not floral initiation 26 , 30 . The genetic flowering pathway of curd induction and formation in response to temperature, especially to high temperature, is still obscure.…”

Section: Introductionmentioning

confidence: 92%

Subtropical adaptation of a temperate plant (Brassica oleracea var. italica) utilizes non-vernalization-responsive QTLs

Lin

JouYi

Tseng

et al. 2018

Sci Rep

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While many tropical plants have been adapted to temperate cultivation, few temperate plants have been adapted to the tropics. Originating in Western Europe, Brassica oleracea vernalization requires a period of low temperature and BoFLC2 regulates the transition to floral development. In B. oleracea germplasm selected in Taiwan, a non-vernalization pathway involving BoFLC3 rather than BoFLC2 regulates curd induction. In 112 subtropical breeding lines, specific haplotype combinations of BoFLC3 and PAN (involved in floral organ identity and a positional candidate for additional curd induction variation) adapt B. oleracea to high ambient temperature and short daylength. Duplicated genes permitted evolution of alternative pathways for control of flowering in temperate and tropical environments, a principle that might be utilized via natural or engineered approaches in other plants. New insight into regulation of Brassica flowering exemplifies translational agriculture, tapping knowledge of botanical models to improve food security under projected climate change scenarios.

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Meristem identity gene expression during curd proliferation and flower initiation in Brassica oleracea

Cited by 44 publications

References 53 publications

Identification of QTLs associated with curd architecture in cauliflower

Identification of QTLs associated with curd architecture in cauliflower

Prediction of flowering time in Brassica oleracea using a quantitative trait loci‐based phenology model

Subtropical adaptation of a temperate plant (Brassica oleracea var. italica) utilizes non-vernalization-responsive QTLs

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