Meristem characteristics of genetically modified pea (<i>Pisum sativum</i>) leaf primordia

Meicenheimer, Roger D.; Muehlbauer, F. J.; Hindman, Joseph L.; Gritton, E. T.

doi:10.1139/b83-386

Cited by 56 publications

(54 citation statements)

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“…This asymmetry (ab-adaxiality) is established early in the leaf primordium and the shoot apical meristem (SAM) seems to provide positional cues for the initial establishment of this asymmetry (Hanawa, 1961;Lynn et al, 1999;Snow and Snow, 1959;Sussex, 1954;Sussex, 1955;Timmermans et al, 1998). Ab-adaxiality defective mutants have been reported in Antirrhinum (Waites and Hudson, 1995), Arabidopsis (Bohmert et al, 1998;Bowman and Smyth, 1999;Chen et al, 1999;Eshed et al, 2001;McConnell and Barton, 1998;Sawa et al, 1999b;Siegfried et al, 1999), maize (Freeling, 1992;Timmermans et al, 1998), Nicotiana (McHale, 1993a;McHale, 1993b;McHale and Marcotrigiano, 1998), tomato (Kessler et al, 2001) and pea (Meicenheimer et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Reduced leaf complexity in tomato wiry mutants suggests a role forPHANandKNOXgenes in generating compound leaves

et al. 2003

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Recent work on species with simple leaves suggests that the juxtaposition of abaxial (lower) and adaxial (upper) cell fates (dorsiventrality) in leaf primordia is necessary for lamina outgrowth. However, how leaf dorsiventral symmetry affects leaflet formation in species with compound leaves is largely unknown. In four non-allelic dorsiventrality-defective mutants in tomato, wiry, wiry3, wiry4 and wiry6, partial or complete loss of ab-adaxiality was observed in leaves as well as in lateral organs in the flower, and the number of leaflets in leaves was reduced significantly. Morphological analyses and expression patterns of molecular markers for ab-adaxiality [LePHANTASTICA (LePHAN) and LeYABBY B (LeYAB B)] indicated that ab-adaxial cell fates were altered in mutant leaves. Reduction in expression of both LeT6 (a tomato KNOX gene) and LePHAN during post-primordial leaf development was correlated with a reduction in leaflet formation in the wiry mutants. LePHAN expression in LeT6 overexpression mutants suggests that LeT6 is a negative regulator of LePHAN. KNOX expression is known to be correlated with leaflet formation and we show that LeT6 requires LePHAN activity to form leaflets. These phenotypes and gene expression patterns suggest that the abaxial and adaxial domains of leaf primordia are important for leaflet primordia formation, and thus also important for compound leaf development. Furthermore, the regulatory relationship between LePHAN and KNOX genes is different from that proposed for simple-leafed species. We propose that this change in the regulatory relationship between KNOX genes and LePHAN plays a role in compound leaf development and is an important feature that distinguishes simple leaves from compound leaves.

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Section: Introductionmentioning

confidence: 99%

Reduced leaf complexity in tomato wiry mutants suggests a role forPHANandKNOXgenes in generating compound leaves

et al. 2003

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“…previously by several authors (Meicenheimer et al, 1983;Gould et al, 1986;DeMason, 1997, 1999a). A brief SEM analysis of near-isogenic lines of these genotypes-JI 1194, JI 1197, JI 1195, and JI 1199, respectively-is presented in Figure 3 to facilitate the identification of those structures labeled in subsequent in situ hybridization sections.…”

Section: Effects Of the Uni Mutation On Leaf Developmentmentioning

confidence: 70%

“…Stipule, leaflet, and tendril primordia are initiated in an acropetal manner on the compound leaf primordium (Meicenheimer et al, 1983), which is termed a marginal blastozone (Hagemann and Gleissberg, 1996). The recessive uni mutation (Eriksson, 1929) reduces the complexity of the pea compound leaf.…”

Section: Pea Leaf Developmentmentioning

confidence: 99%

Pea Compound Leaf Architecture Is Regulated by Interactions among the Genes UNIFOLIATA, COCHLEATA, AFILA, and TENDRIL-LESS

2000

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The compound leaf primordium of pea represents a marginal blastozone that initiates organ primordia, in an acropetal manner, from its growing distal region. The UNIFOLIATA ( UNI ) gene is important in marginal blastozone maintenance because loss or reduction of its function results in uni mutant leaves of reduced complexity. In this study, we show that UNI is expressed in the leaf blastozone over the period in which organ primordia are initiated and is downregulated at the time of leaf primordium determination. Prolonged UNI expression was associated with increased blastozone activity in the complex leaves of afila ( af ), cochleata ( coch ), and afila tendril-less ( af tl ) mutant plants. Our analysis suggests that UNI expression is negatively regulated by COCH in stipule primordia, by AF in proximal leaflet primordia, and by AF and TL in distal and terminal tendril primordia. We propose that the control of UNI expression by AF , TL , and COCH is important in the regulation of blastozone activity and pattern formation in the compound leaf primordium of the pea.

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“…Many mutations affecting the process of leaf development have been described (Marx, 1983;Sinnott, 1960;Caruso, 1968;Meicenheimer et al, 1983). For example, mutations at the phantastica (phan) locus of Antirrhinum majus show loss of the dorsoventrality in leaves.…”

Section: Introductionmentioning

confidence: 99%

Dorsoventral pattern formation of tobacco leaf involves spatial expression of a tobacco homeobox gene, NTH15.

1997

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Biological function of a tobacco homeobox gene, NTH15, was studied by producing two different types of transgenic tobacco, one of which was transformed with NTH15::GUS and the other was transformed with CaMV 35S promoter::antisense NTH15. The GUS staining around shoot apical meristem (SAM) in transgenic plants with NTH15::GUS revealed that the NTH15 expression is specifically localized in the dorsal region of young leaf primordia. The transgenic plants transformed with the antisense construct lost the expression of the endogenous transcript around SAM and also showed alterations in leaf morphology. These transformants formed leaves with disorganized pinnate venation. Midribs of the malformed leaves also lost crescent-shaped vascular systems by ectopic cell vacuolization in their adaxial side. These observations suggest suppression of the NTH15 expression around SAM causes loss of dorsoventrality in midribs. Taken together with the observations of the NTH15 expression pattern, it may be important for the dorsoventral development of leaves that the NTH15 gene is expressed in the adaxial side of young leaf primordia.

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Meristem characteristics of genetically modified pea (Pisum sativum) leaf primordia

Cited by 56 publications

References 0 publications

Reduced leaf complexity in tomato wiry mutants suggests a role forPHANandKNOXgenes in generating compound leaves

Reduced leaf complexity in tomato wiry mutants suggests a role forPHANandKNOXgenes in generating compound leaves

Pea Compound Leaf Architecture Is Regulated by Interactions among the Genes UNIFOLIATA, COCHLEATA, AFILA, and TENDRIL-LESS

Dorsoventral pattern formation of tobacco leaf involves spatial expression of a tobacco homeobox gene, NTH15.

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