Meristem and Shoot‐Tip Culture for Propagation, Pathogen Elimination, and Germplasm Preservation

Styer, D. J.; Chin, Chee‐Kok

doi:10.1002/9781118060728.ch5

Cited by 11 publications

(9 citation statements)

References 186 publications

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“…The recovery of the plant growth suggests that the number of virus particles was decreased or eliminated. Therefore, explants are optimally able to absorb nutrients from the media and maximize metabolic processes to synthesize the elements needed for vegetative growth (Styer and Chin, 1983).…”

Section: Tection Using Rt-pcrmentioning

confidence: 99%

Elimination of shallot bulb viruses through heat treatment

2017

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Shallot (Allium cepa L. Aggregatum group) is usually cultivated vegetatively. As a result, viruses tend to accumulate within the host plants and spread to healthy plants every crop cycle, reducing yield and bulb quality. There are a very limited number of studies about the elimination of shallot viruses through heat treatment. The objective of this research was to eliminate shallot viruses through heat treatment to produce virus-free plantlets.The leaves of Biru Lancor with specific visual virus symptoms were detected by Reverse TranscriptionPolymerase Chain Reaction (RT-PCR). Then bulbs of Biru Lancor that were positively infected by viruses were used as materials for heat treatment. The treatments were a control (without treatment), electric treatment at 15 mA for 10 minutes, heat treatment in an incubator at 37°C for 4 weeks, heat treatment in a waterbath at 45°C for 60 minutes, and combination of heat treatment in an incubator at 37°C for 4 weeks and heat treatment in a waterbath at 45°C for 60 minutes. After being subjected to heat treatment, the pseudo stem were cultivated in the MS Medium + 1 mg/L BAP + 1 mg/L IBA.Virus detection by RT-PCR was conducted 28 days after planting using samples of leaves from each plantlet.The results of this research showed that the treatments of electric treatment at 15 mA for 10 minutes and combination of heat treatment in the incubator at 37°C for 4 weeks and heat treatment in the waterbath at 45°C for 60 minutes could suppress the incidence of Shallot latent virus (SLV) until 100%. Heat treatment might have an important role in the degradation of virus particles by boosting Virus-Induced Gene Silencing (VIGS) as plant responses to virus infection.

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Section: Tection Using Rt-pcrmentioning

confidence: 99%

Elimination of shallot bulb viruses through heat treatment

2017

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“…And, the shoot-tip explants are usually taken from the tender tip of the growing shoot measuring 2 cm or less in length. Styer and Chin (1983) stated that all explants from shoot tips differ in size and in the number of leaf primordia, and apical meristerns without any leaf primordia have the highest probability of producing plants lacking detectable levels of viruses, and thus, they are the explants most often used for the production of. disease-indexed plants.…”

Section: Discovery Of Growth Regulatorsmentioning

confidence: 99%

“…In general, a wide range of light intensities is suitable for various cultures. Intensities below 1,000 lux are used only occasionally, while intensities between 1,000 and 4,000 lux are the most common (Styer and Chin 1983). Light intensity has been shown to affect the type of growth in culture.…”

Section: ) Light Intensitymentioning

confidence: 99%

“…10,000 lux were requi red before being transferred to soil to enhance their ability to survive in the greenhouse (Murashige 1974, Hughes 1982, Styer and Chin 1983, and Hasegawa et . §l.…”

Section: ) Light Intensitymentioning

confidence: 99%

“…He also stated that the best photoperiod for a given tissue culture is dependent upon the light used, and probably other factors. Many workers have commomly used 14 to 18 hr photoperiods for tissue cultures (Styer and Chin 1983, Dabiski et al 1979, Kerbauy 1984, Stoltz 1984, Blazich and Novitzky 1984, Stephens et ..Q.l. 1985, Ettinger and Preece 1985, and Econanou and Spanoudaki 1985.…”

Section: ) Light Intensitymentioning

confidence: 99%

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