Merging methods in molecular and ecological genetics to study the adaptation of plants to anthropogenic metal‐polluted sites: implications for phytoremediation

Pauwels, Maxime; Willems, Glenda; Roosens, Nancy; Frérot, Hélène; Saumitou‐Laprade, Pierre

doi:10.1111/j.1365-294x.2007.03486.x

Cited by 44 publications

(37 citation statements)

References 73 publications

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“…Interestingly, we found M populations within two chlorotype lineages while, for example, in Arabidopsis halleri, a model pseudometallophyte, all M populations, of independent origins, come from one chlorotype lineage only (north of the Alps) (Pauwels et al 2005(Pauwels et al , 2008. Cluster 5 (EMA) Fig.…”

Section: Discussionmentioning

confidence: 92%

Chloroplast microsatellites reveal that metallicolous populations of the Mediterranean shrub Cistus ladanifer L have multiple origins

Quintela-Sabarís

Vendramin²,

Castro-Fernández

et al. 2010

Plant Soil

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Cistus ladanifer L. (Cistaceae) is a Mediterranean shrub covering different kinds of soils in the Western Mediterranean area. This species has colonised several metalliferous areas (serpentine outcrops as well as human-polluted sites) throughout its distribution range, and is therefore an interesting species to study the possible effects on genetic diversity and differentiation produced by the colonisation of areas polluted with heavy metals. The genetic structure of 33 natural populations distributed across its entire natural distribution range (Morocco, Portugal and Spain) and growing on either metalliferous or non-metalliferous soils was investigated using chloroplast microsatellites. Population genetic parameters were estimated and genetic groups were identified using Bayesian inference. In addition, we compared the genetic diversity and differentiation among metallicolous and non-metallicolous populations within each Bayesian-defined group. The cpSSR data suggested that metallicolous populations of Cistus ladanifer have arisen through multiple independent evolutionary origins within two different chloroplast lineages. Evidence that the soil type provoked genetic bottlenecks in metallicolous populations or genetic differentiation among metallicolous and non-metallicolous populations was not observed. Historical factors are the main cause of the present genetic structure of C. ladanifer. The nature of tolerance to heavy metals as a species-wide trait in this shrub is discussed.

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Section: Discussionmentioning

confidence: 92%

Chloroplast microsatellites reveal that metallicolous populations of the Mediterranean shrub Cistus ladanifer L have multiple origins

Quintela-Sabarís

Vendramin²,

Castro-Fernández

et al. 2010

Plant Soil

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“…For the analysis of the presence of all the MTP1 paralogues in accessions representing the genetic diversity within the A. halleri species, one plant was taken at random from each of the following populations: M Auby from France, M Sauerland, NM Bavarian Forest and M Harz from Germany, NM CZ8-13 from Czech Republic, NM Nord Tyrol from Austria, M Katowice-Weinowice and NM Zakopane from Poland, NM Apuseni mountains, NM Fagaras Ro-12-6 and NM Fagaras Ro-ovirensis from Romania, NM Southern Tyrol, M Lombardie and NM Tessin from Italy, where M qualifies a metallicolous population and NM a non metallicolous one according to already described criteria [13].…”

Section: Methodsmentioning

confidence: 99%

“…In particular, populations collected on metalliferous soils (known as metallicolous populations) display enhanced zinc tolerance compared to populations collected in metal uncontaminated sites (known as non metallicolous populations) [11]. Interestingly, a phylogeographic survey proposed that geographically distant metallicolous populations have been founded independently in distinct polluted areas [13] suggesting that the adaptive improvement of zinc tolerance may involve distinct genetic mechanisms in distinct metallicolous populations.…”

Section: Introductionmentioning

confidence: 99%

The Five AhMTP1 Zinc Transporters Undergo Different Evolutionary Fates towards Adaptive Evolution to Zinc Tolerance in Arabidopsis halleri

et al. 2010

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Gene duplication is a major mechanism facilitating adaptation to changing environments. From recent genomic analyses, the acquisition of zinc hypertolerance and hyperaccumulation characters discriminating Arabidopsis halleri from its zinc sensitive/non-accumulator closest relatives Arabidopsis lyrata and Arabidopsis thaliana was proposed to rely on duplication of genes controlling zinc transport or zinc tolerance. Metal Tolerance Protein 1 (MTP1) is one of these genes. It encodes a Zn2+/H+ antiporter involved in cytoplasmic zinc detoxification and thus in zinc tolerance. MTP1 was proposed to be triplicated in A. halleri, while it is present in single copy in A. thaliana and A. lyrata. Two of the three AhMTP1 paralogues were shown to co-segregate with zinc tolerance in a BC1 progeny from a cross between A. halleri and A. lyrata. In this work, the MTP1 family was characterized at both the genomic and functional levels in A. halleri. Five MTP1 paralogues were found to be present in A. halleri, AhMTP1-A1, -A2, -B, -C, and -D. Interestingly, one of the two newly identified AhMTP1 paralogues was not fixed at least in one A. halleri population. All MTP1s were expressed, but transcript accumulation of the paralogues co-segregating with zinc tolerance in the A. halleri X A. lyrata BC1 progeny was markedly higher than that of the other paralogues. All MTP1s displayed the ability to functionally complement a Saccharomyces cerevisiæ zinc hypersensitive mutant. However, the paralogue showing the least complementation of the yeast mutant phenotype was one of the paralogues co-segregating with zinc tolerance. From our results, the hypothesis that pentaplication of MTP1 could be a major basis of the zinc tolerance character in A. halleri is strongly counter-balanced by the fact that members of the MTP1 family are likely to experience different evolutionary fates, some of which not concurring to increase zinc tolerance.

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“…Pollution stress may affect allocation of resources within the affected plants (Lechowitz 1987) and provide molecular signals for differential gene expression (Pouwels et al 2008). These changes may induce phenotypic differences, referred to as adaptive phenotypic plasticity (reviewed in Barrett & Bush 1991) and recently connected to epigenetic effects (Yakovlev et al 2010).…”

Section: Var(c)mentioning

confidence: 99%

A fast screening approach for genetic tolerance to air pollution in Scots pine field tests

Danusevičius¹,

Marozas²,

Augustaitis³

et al. 2013

iForest

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© iForest -Biogeosciences and Forestry IntroductionClimatic chamber testing is the most efficient approach to identify pollution tolerant genotypes (e.g., Oleksyn & Bialobok 1986). However, limited space and high costs of maintaining the indoor tests allow testing a restricted number of genetic entries. A prescreening for tolerance under natural conditions could raise the efficiency of the indoor tests by pre-identifying the tolerant and nontolerant genotypes. Genetic field tests affected by air pollution could provide additional information on the genetic variation in the traits connected to air pollution tolerance. In our study, we benefited from a genetic test (clonal seed orchard) established nearby a nitrogen fertilizer plant and markedly affected by its emissions mainly during a major accident in the fertilizer plant .Being an issue of high public concern, a large number of studies on the effects of pollution to tree growth and tolerance mechanisms were carried out (e.g., Cape et al. 1989, Crossley & Fowler 1986, Tuomisto 1988, Kupcinskiene 2000. Though the genetic tolerance experiments are less abundant, there is a strong indication of a genetic component in the pollution stress tolerance (Bergmann & Scholz 1987b, Korshikov et al. 2002, Prus-Glowacki et al. 2006. Several independent studies reported a positive relationship between the genetic polymorphism of trees and their tolerance to air pollution (Geburek et al. 1987, Oleksyn et al. 1994, Prus-Glowacki et al. 2006). There was a significant Scots pine provenance variation in tolerance to air pollution in the vicinity of a nitrogen fertilizer plant in Poland (Oleksyn et al. 1994). Radial increment together with crown defoliation proved to be appropriate indicators of pollution tolerance in Scots pine genetic field tests (Oleksyn et al. 1994 The objective of our study was to develop a screening approach for genetic tolerance to industrial pollution in Scots pine. We assessed the relationship between the temporal variation in strength of genetic control of radial increment of the seed orchard clones and past pollutant emissions. The genetic relationship between past radial increment and present-day defoliation as well as tree diameter of the seed orchard clones was also investigated. Materials and methodsThe clonal seed orchard was located 12 km north-eastwards from the JV nitrogen fertilizer plant in central Lithuania (latitude 55° 06', longitude 24° 26', elevation 77 m a.s.l.). It consisted of 28 clones with 20 ramets each and was established in 1972 with local genetic material. The origin of the root stocks is unknown. The spacing between the clones was 8 x 8 meters. The design of the orchard was 28 tree blocks containing one individual of each clone arranged in sequential order with five clones within each row. The seed orchard was not thinned. In 2011, increment cores were sampled from each ramet of the 28 clones. At the same time, the following traits reflecting present-day health and genetic differences among the clones were assessed: tree diameter...

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Merging methods in molecular and ecological genetics to study the adaptation of plants to anthropogenic metal‐polluted sites: implications for phytoremediation

Cited by 44 publications

References 73 publications

Chloroplast microsatellites reveal that metallicolous populations of the Mediterranean shrub Cistus ladanifer L have multiple origins

Chloroplast microsatellites reveal that metallicolous populations of the Mediterranean shrub Cistus ladanifer L have multiple origins

The Five AhMTP1 Zinc Transporters Undergo Different Evolutionary Fates towards Adaptive Evolution to Zinc Tolerance in Arabidopsis halleri

A fast screening approach for genetic tolerance to air pollution in Scots pine field tests

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