Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region

Vieira, José Cavalcante Souza; Braga, Camila Pereira; Oliveira, Grasieli de; Padilha, Cilene do Carmo Federici; Moraes, Paula Martin de; Zara, Luiz Fabrício; Leite, Aline de Lima; Buzalaf, Marília Afonso Rabelo; Padilha, Pedro de Magalhães

doi:10.1007/s12011-017-1129-5

Cited by 20 publications

(8 citation statements)

References 30 publications

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“…Besides, in jaraqui ( Semaprochilodus spp. ), a species of Amazonian fish, researchers found approximately two atoms of Hg per RPS27a molecule in hepatic tissue (J. C. S. Vieira et al, 2018). Moreover, in the hepatic and muscular tissue of filhote ( Brachyplatystoma filamentosum ), a giant catfish in the Amazonian rivers, Hg can also bind to RPS27a (de Queiroz et al, 2019).…”

Section: Rps27a: a Mercury Carrier And Biomarkermentioning

confidence: 99%

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Multifaceted functions of RPS27a: An unconventional ribosomal protein

Luo

Zhao

Chen

et al. 2022

Journal Cellular Physiology

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The ribosomal protein S27a (RPS27a) is cleaved from the fusion protein ubiquitin–RPS27a (Ub–RPS27a). Generally, Ub and RPS27a are coexpressed as a fusion protein but function independently after Ub is cleaved from RPS27a by a deubiquitinating enzyme. As an RP, RPS27a assembles into ribosomes, but it also functions independently of ribosomes. RPS27a is involved in the development and poor prognosis of various cancers, such as colorectal cancer, liver cancer, chronic myeloid leukemia, and renal carcinoma, and is associated with poor prognosis. Notably, the murine double minute 2/P53 axis is a major pathway through which RPS27a regulates cancer development. Moreover, RPS27a maintains sperm motility, regulates winged aphid indirect flight muscle degeneration, and facilitates plant growth. Additionally, RPS27a is a metalloprotein and mercury (Hg) biomarker. In the present review, we described the origin, structure, and biological functions of RPS27a.

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Section: Rps27a: a Mercury Carrier And Biomarkermentioning

confidence: 99%

“…Besides, in jaraqui (Semaprochilodus spp. ), a species of Amazonian fish, researchers found approximately two atoms of Hg per RPS27a molecule in hepatic tissue (J. C. S Vieira et al, 2018)…”

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confidence: 99%

Multifaceted functions of RPS27a: An unconventional ribosomal protein

Luo

Zhao

Chen

et al. 2022

Journal Cellular Physiology

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“…Uma importante aplicabilidade desta técnica é a identificação e caracterização de potenciais biomarcadores, como por exemplo, proteínas associadas ao mercúrio e zinco. [79][80][81][82][83] Em função de seu tamanho e carga, as moléculas vão se mover através do gel em diferentes direções ou em diferentes velocidades, o que permite que sejam separadas umas das outras. As moléculas maiores na amostra se movem mais lentamente que as menores, de modo que moléculas de vários tamanhos moleculares se separam em faixas distintas no meio de gel.…”

Section: Eletroforese Em Gelunclassified

Metallomics: Methodologies and Applications in Environmental Studies

Silva¹,

Silva²

2020

Rev. Virtual Quim.

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Metallomics is an interdisciplinary area that has grown in recent years thanks to the development and application of techniques such as electrophoresis, chromatography, and spectrometry. Advances in this area, have allowed studies of functional and structural characterization of proteins linked to metals in different organisms and tissues, and thus, led to the understanding of the complex processes involving metalloproteins, and with this, their application for the realization of environmental monitoring studies. In this article, a review of the main metallomic techniques and their applications in environmental studies was carried out from 2008 to 2018, based on the search for scientific articles in the "Scopus" database. A metalômica é uma área interdisciplinar que tem crescido nos últimos anos, em virtude ao desenvolvimento e aplicação de técnicas como eletroforese, cromatografia e espectrometria. Avanços nessa área, têm permitido estudos de caracterização funcional e estrutural de proteínas ligadas a metais em diferentes organismos e tecidos, e desta forma, levado à compreensão, dos complexos processos que envolvem as metaloproteínas, e com isto, garantido sua aplicação para a realização de estudos de monitoramento ambiental. No presente artigo, foi realizada uma revisão das principais técnicas metalômicas e de suas aplicações em estudos ambientais no período de 2008 a 2018, a partir da busca de artigos científicos na base de dados "Scopus".

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“…The fish species Brachyplatystoma filamentosum is also the most consumed by the riverside population of the Madeira River. Thus, consumption of Brachyplatystoma filamentosum may pose a risk to the health of these coastal communities from the Brazilian Amazon [3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…This new area of scientific knowledge has contributed to elucidating the physiological and functional aspects of proteins responsible for transporting metal ions in living organisms [8]. The elucidation of mercurial species complexed by proteins and enzymes using the metallomics allow detailed study of an increase or decrease in the expression of these metalloproteins, compared to changing limnological characteristics and the availability of mercury species in the environment of the Amazon Rivers [2,4,9]. Thus, metalloproteins and metal-binding proteins characterized under these study conditions may be used as biomarkers of mercury exposure in fish from the Amazon region.…”

Section: Introductionmentioning

confidence: 99%

Identification of Biomarkers of Mercury Contamination in Brachyplatystoma filamentosum of the Madeira River, Brazil, Using Metalloproteomic Strategies

Queiroz

Vieira

Oliveira

et al. 2018

Biol Trace Elem Res

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Predator fish can accumulate high levels of mercury, which qualifies them as potential indicators of this toxic metal. The predatory species Brachyplatystoma filamentosum, popularly known as filhote, is among the most consumed species in the Brazilian Amazon. Continuing the metalloproteomic studies of mercury in Amazonian fishes that have been developed in the last 5 years, the present paper provides the data of protein characterization associated with mercury in muscle and liver samples of filhote (Brachyplatystoma filamentosum) collected in the Madeira River, Brazilian Amazon. The mercury concentration in the muscle and liver samples was determined by graphite furnace atomic absorption spectrometry (GFAAS). The protein fraction was extracted in an aqueous medium, and later, a fractional precipitation procedure was performed to obtain the protein pellets. Then, the proteome of the tissue samples of this fish species was separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and a mercury mapping of the protein spots was carried out by GFAAS after acid digestion. Protein spots that had mercury were characterized by mass spectrometry with electrospray ionization in sequence (ESI-MS/MS) after tryptic digestion. It was possible to characterize 11 mercury-associated protein spots that presented biomarker characteristics and could be used to monitor mercury in fish species of the Amazon region. Thus, the metalloproteomic strategies used in the present study allowed us to characterize 11 mercury-associated protein spots. It should be noted that the protein spots identified as GFRP, TMEM186, TMEM57B, and BHMT, which have coordination sites for elements with characteristics of soft acids, such as mercury, can be used as biomarkers of mercury contamination in monitoring studies of this toxic metal in fish species from the Amazon region.

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Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region

Cited by 20 publications

References 30 publications

Multifaceted functions of RPS27a: An unconventional ribosomal protein

Multifaceted functions of RPS27a: An unconventional ribosomal protein

Metallomics: Methodologies and Applications in Environmental Studies

Identification of Biomarkers of Mercury Contamination in Brachyplatystoma filamentosum of the Madeira River, Brazil, Using Metalloproteomic Strategies

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