Mercury determination by FI-CV-AAS after the degradation of organomercurials with the aid of an ultrasonic field: The important role of the hypochlorite ion

Capelo, José Luís; Rivas, Maria G.; Oliveira, Leônidas; Vilhena, C.; Santos, Ana Paula de Oliveira; Valada, Tatiana; Galesio, M.; Oliveira, Paulo Roberto de; Silva, M.D.R. Gomes da; Gaspar, E.; Alves, Sheila; Fernandez, C.; Vaz, Carlos

doi:10.1016/j.talanta.2005.06.004

Cited by 24 publications

(14 citation statements)

References 6 publications

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“…The mercury line at 253.7 nm and a slit width of 0.5 nm were used for measurements. The mercury adsorbed to the biomass was determined using a modification to the method described by Capelo et al [19]. In this procedure, the solution was composed of 2 M HCl, 3% (v v −1 ) NaClO and the sludge sample, and stored in a closed vessel for 24 h. This solution, containing the Hg in both the liquid and solid phases, was then analysed by AAS, as described above.…”

Section: Mercury Analysis In the Liquid And Solid Phasesmentioning

confidence: 99%

“…However, the analytical technique used for analysis of Hg in the solid + liquid phases [19] also incorporates the analysis of organomercurials such as MeHg. One critical component of the analytical procedure necessary for MeHg detection is ultrasonication.…”

Section: Methylmercury Analysismentioning

confidence: 99%

“…One critical component of the analytical procedure necessary for MeHg detection is ultrasonication. Samples were tested using an identical procedure as described in Capelo et al [19] with and without sonication, in order to detect if MeHg was produced by the mixed cultures.…”

Section: Methylmercury Analysismentioning

confidence: 99%

“…Furthermore, the potential of MeHg formation by the mixed cultures was evaluated (see Section 2.5.3), where ultrasonication was employed (according to [19]) to detect if MeHg was formed in samples from each culture. However, it was found that the total Hg concentrations in the solid and liquid phases were virtually identical with and without sonication (data not shown).…”

Section: Mercury Removal In Mixed Microbial Culturesmentioning

confidence: 99%

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The effect of carbon source on the biological reduction of ionic mercury

Oehmen

Fradinho

Serra

et al. 2009

Journal of Hazardous Materials

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Section: Mercury Analysis In the Liquid And Solid Phasesmentioning

confidence: 99%

Section: Methylmercury Analysismentioning

confidence: 99%

Section: Methylmercury Analysismentioning

confidence: 99%

Section: Mercury Removal In Mixed Microbial Culturesmentioning

confidence: 99%

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The effect of carbon source on the biological reduction of ionic mercury

Oehmen

Fradinho

Serra

et al. 2009

Journal of Hazardous Materials

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“…Conventional analytical methods for detecting mercury are based on atomic absorption spectrometry (AAS) and atomic fluorescence spectrometry (AFS) integrated with cold vapor generation system [5][6][7]. However, the limitation of complicated and expensive instrumentation cannot be ignored, as well as enzyme-linked immunosorbent assays (ELISA) [8], fluorescent detection [9,10].…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive electroluminescence immunoassay for Hg(II) ions based on the use of CdSe quantum dots, the methylmercury-6-mercaptonicotinic acid-ovalbumin conjugate, and a specific monoclonal antibody

et al. 2014

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We have designed a rapid and ultrasensitive electrochemiluminescent (ECL) competitive immunoassay for the determination of mercury(II) ion. It is based on the use of CdSe quantum dots (QDs), methylmercury-6-mercaptonicotinic acid-ovalbumin as coating antigen and specific monoclonal antibodies (mAbs) against Hg(II). The latter is quite selective for Hg(II). The coating antigen was immobilized on the surface of a gold electrode via reaction between the functional groups of cysteamine and glutaraldehyde. The mercury(II) ions in a sample and the coating antigen compete for binding sites of QD-labeled monoclonal antibody which binds specifically to Hg(II) ions. The ECL of the system decreases with increasing concentration of Hg(II) because less QD-labeled mAbs are present on the surface of the electrode. Under optimal conditions, the decrease of ECL intensity is linearly related to the logarithm of the Hg(II) concentration in the range from 0.02 to 100 ng mL −1 , with a detection limit of 6.2 pg mL −1 . As far as we know, this is the first report on an ECL immunoassay for Hg(II) based on a specific monoclonal antibody. The favorable results obtained when this method was applied to real samples indicate that this detection scheme can widely enlarge the applicability of detecting heavy metal ions by exploiting the ECL of QDs for immunoassays.

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