Mercuric-Ion-Induced Gene Expression in<i>Arabidopsis thaliana</i>

Heidenreich, Bernd; Seidlitz, Harald K.; Ernst, Dieter; Sandermann, Heinrich

doi:10.1080/15226519908500013

Cited by 11 publications

(18 citation statements)

References 46 publications

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“…Similarly, stress‐induced cDNA libraries have been constructed from short‐term exposed plant material. In Arabidopsis thaliana , long‐term growth on mercuric chloride resulted in an enhanced level of metallothionein mRNA, whereas pathogenesis‐related protein transcripts could not be detected (Heidenreich et al . 1999).…”

Section: Introductionmentioning

confidence: 99%

Mercury‐induced genes in Arabidopsis thaliana: identification of induced genes upon long‐term mercuric ion exposure

Heidenreich¹,

Mayer²,

Sandermann³

et al. 2001

Plant Cell & Environment

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Mercuric-ion-induced gene expression was studied in Arabidopsis thaliana Columbia wild type. Rosettes of plants grown for 21 d on agar medium supplemented with 20, 30 and 40 µ µ µ µ M HgCl 2 were pooled and used to isolate cDNAs of induced genes by suppression subtractive hybridization. Of the 576 clones isolated initially, 31 turned out to be mercury-induced by Northern hybridization. However, kinetic studies using cDNA arrays clearly showed that seven genes were exclusively mercuric-ion-induced, 14 were induced by mercury but also affected by a diurnal rhythm, and 10 clones were only modulated by the day-night cycle. The expression levels of the metal-induced genes increased from 1·5-fold to 10-fold. Functional classification resulted in genes encoding proteins for the photosynthetic apparatus and for the antioxidative system. In addition, unexpected genes, whose connection to mercury ion stress is not evident, were identified.

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Section: Introductionmentioning

confidence: 99%

Mercury‐induced genes in Arabidopsis thaliana: identification of induced genes upon long‐term mercuric ion exposure

Heidenreich¹,

Mayer²,

Sandermann³

et al. 2001

Plant Cell & Environment

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“…This overlap is not surprising considering that in plants stress related transcripts are often not speciWc and they are likely to be aVected by other stress factors such as heavy metals and salts (Heidenreich et al 1999;Reymond et al 2000;Brosche et al 2002). This could be due to cross-talk of diVerent stress factors leading to activation of identical genes (Sandermann et al 1998).…”

Section: Discussionmentioning

confidence: 81%

“…The discovery of genes for phytoremediation may beneWt from the naturally selected metal hyperaccumulation in plants (Krä-mer 2005). The identiWcation of gene transcripts regulated diVerentially by heavy-metal-induced stress can provide clues to stress networks inXuenced by other factors such as ozone, UV-B radiation, heavy metal and elicitor treatments (Heidenreich et al 1999;Langebartels et al 2000;Logemann et al 2000;Säven-strand et al 2000). The SSH is a powerful technique that enables cloning of ESTs representing genes that are diVerentially expressed in two diVerent populations.…”

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confidence: 98%

Identification of lead-regulated genes by suppression subtractive hybridization in the heavy metal accumulator Sesbania drummondii

Srivastava

Venkatachalam

Raghothama

et al. 2006

Planta

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Heavy metal contamination of soils is of widespread occurrence as a result of human, agricultural and industrial activities. Among heavy metals, lead is a potential pollutant that readily accumulates in soils and sediments. Although lead is not an essential element for plants, it gets easily absorbed and accumulated in Sesbania drummondii, which exhibits a significant level of tolerance to lead. The response of a metal tolerant plant to heavy metal stress involves a number of biochemical and physiological pathways. To investigate the overall molecular response of a metal-tolerant plant to lead exposure, suppression subtractive hybridization (SSH) was used to construct a cDNA library enriched in lead induced mRNA transcripts from lead-tolerant Sesbania. Screening the library by reverse Northern analysis revealed that between 20 and 25% of clones selected from the library were differentially regulated in lead treated plants. After differential screening, we isolated several differentially expressed cDNA clones, including a type 2 metallothionein (MT) gene which is involved in detoxification and homeostasis and shown to be differentially regulated in lead treated plants. The data from the reverse Northern analysis was further confirmed with conventional Northern analysis of a select group of genes including MT, ACC synthase/oxidase, cold-, water stress-, and other abiotic stress-induced genes, which are up-regulated rapidly in response to lead treatment. The mRNA levels of MT increased substantially after lead treatment indicating a potential role for it under lead stress in Sesbania. The present results show that SSH can serve as an effective tool for isolating genes induced in response to lead heavy metal tolerance in Sesbania. A better understanding of lead induced gene expression in Sesbania should help select candidates associated with remediation of heavy metal toxicity. The possible link between this result and the heavy-metal response of plants is discussed.

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“…Furthermore, the uptake of Hg(II) by the roots may be restricted as concluded from flux measurements on xylem sap of forest-grown pine and spruce with background Hg exposure (Bishop et al 1998). In contrast, experiments with 1-year-old aspen growing under controlled-environment conditions on soil of high Hg concentration (£ 12 mg kg -1 ) support the assumption that plants may act as a conduit for Hg to move from soil into the air (Ericksen & Gustin 2004), and studies with Arabidopsis thaliana grown on agar supplemented with 8 mg Hg(II) L -1 showed a leaves/medium bioconcentration up to 12 (based on leaves' wet weight; Heidenreich et al 1999). Thus, the transport of mercury with the transpiration flow appears to depend on the plant species and on the external Hg(II) concentration near the roots.…”

Section: Discussionmentioning

confidence: 96%

“…1998). In contrast, experiments with 1‐year‐old aspen growing under controlled‐environment conditions on soil of high Hg concentration (≤ 12 mg kg −1 ) support the assumption that plants may act as a conduit for Hg to move from soil into the air (Ericksen & Gustin 2004), and studies with Arabidopsis thaliana grown on agar supplemented with 8 mg Hg(II) L −1 showed a leaves/medium bioconcentration up to 12 (based on leaves’ wet weight; Heidenreich et al . 1999).…”

Section: Discussionmentioning

confidence: 99%