Mercuric chloride releases preformed mediators from mast cells of the mouse and rat

Dastych, Jarosław; Kolago, B; Michoń, T; Zwolinska, Anna; Wyczółkowska, J

doi:10.1007/s000110050386

Cited by 2 publications

(1 citation statement)

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“…Previous studies with HgCl 2 have shown either enhancement of mediator release from activated rat peritoneal mast cells (Oliveira et al 1995;Wolfreys and Oliveira 1997), direct activation of rat and murine mast cells (Dastych et al 1999a, b), or an inhibitory action on mast cell activation in the HMC-1 cell line (Wabrick et al 1995). These differences were explained as possible effects associated with different experimental conditions.…”

Section: Discussionmentioning

confidence: 96%

Effect of methylmercury on histamine release from rat mast cells

Graevskaya

Yasutake

Aramaki

et al. 2003

Archives of Toxicology

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Methylmercury chloride (MeHgCl) is well known as a significant environmental hazard, particularly as a modulator of the immune system. As it is acknowledged that the critical effector cells in the host response participating in various biological responses are mast cells, we tried to define the possible contribution of mast cells in the development of methylmercury-evoked effects. We investigated the effects of methylmercury on the rat mast cell degranulation induced by non-immunological stimuli (the selective liberator of histamine, compound 48/80, and calcium ionophore A23187) both in vivo and in vitro. Using the cells prepared from methylmercury-intoxicated rats through a 5-day treatment of MeHgCl (10 mg/kg/day), we observed the suppression of calcium ionophore A23187- and 48/80-induced histamine release, which was enhanced with time after treatment. Similar suppression was observed in the ionophore-stimulated release, when cells were prepared from rat with a single treatment of MeHgCl (20 mg/kg). It should be noted that when cells from the control rat were pre-incubated with methylmercury in vitro at a 10(-8) M concentration for 10 min, A23187 and compound 48/80-stimulated histamine release was significantly enhanced. However, when the pre-incubation period was prolonged to 30 min, the release was suppressed. An increase in the methylmercury concentration to 10(-6) M also suppressed the histamine release. These results show that methylmercury treatment can modify mast cell function depending on concentration and time, and might provide an insight into the role of mast cells in the development of methylmercury-stimulated effects.

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