Mercaptophenylboronic Acid-Activated Gold Nanoparticles as Nanoantibiotics against Multidrug-Resistant Bacteria

Abstract

Multidrug-resistant (MDR) bacteria-induced infections are becoming challenging issues threatening human health and life. Current antibiotics can hardly tackle this problem. Herein, we present a strategy to prepare mercaptophenylboronic acid (MBA)activated gold nanoparticles (Au NPs) as an antibacterial agent against MDR bacteria. Both Au NPs and MBA cannot serve as antibiotics. However, when MBA attaches on Au NPs, the Au_MBA NPs show potent antibacterial activities against Gram-positive MDR clinical isolates (e.g., MDR Staphyloccocus aureus, MDR S. aureus; MDR Staphyloccocus epidermidis, MDR S. epidermidis). Furthermore, Au_MBA NPs show an extremely high median lethal dose (LD 50,i.v., 960 mg/kg), which is much higher than those of most of the clinically used antibiotics. As an application example, we dope Au_MBA NPs with electrospun poly(εcaprolactone) (PCL)/gelatin nanofibrous membranes as wound dressings, which show striking ability to remedy S. aureus-or MDR S. aureus-infected full-thickness skin wounds on rats. Our study provides a novel strategy for treating MDR bacteria-infected wounds in a simple, low-cost, and efficient way, which holds promise for broad clinical applications.

Nanofibrous dressing: Potential alternative for fighting against antibiotic‐resistance wound infections

“…Wang et al 84 modified Au NPs with mercaptophenyl boronic acid (MBA) through the sulfhydryl group and the boronic acid group. The PCL/PG/Au‐MBA electrospun membrane showed an apparent growth inhibition zone after contacting with Gram‐positive bacteria S. aureus and MRSA for 24 h. It was also used to treat rat wounds with S. aureus and DR S. aureus infections, on the 14th day after the operation, the wound was covered by new tissue, and the wound healing rate reached 98 ± 3%.…”

Nanomaterials for the Diagnosis and Treatment of Urinary Tract Infections

“…Compared with conventional antimicrobials, NP-based antimicrobials are easy to fabricate and display prolonged stability, sustained drug release, and higher therapeutic efficacy. NPs that possess antimicrobial properties themselves or increase the therapeutic efficiency of antibiotics are referred nanoantibiotics [112]. Nanoantibiotics kill pathogens through multiple mechanisms including via formation of ROS, interference with energy transduction pathway, inhibition of DNA synthesis, and degradation of the microbial cell wall [113].…”

Functional nanoparticles in electrospun fibers for biomedical applications

“…[ 48 ] In a recent study conducted by Wang et al., mercaptophenylboronic acid activated Au NPs were doped onto electrospun poly (ε‐caprolactone) (PCL)/gelatin nanofibrous membranes for designing multidrug resistant wound dressing. [ 49 ] The authors synthesized the Au NPs through one‐pot synthesis method and tested their antibacterial activities in vitro. Surprisingly, the median lethal dose (LD 50) of mercaptophenylboronic acid activated Au NPs reached to 960 mg kg ‐1 , way higher than the clinical dosage of most antibiotics.…”