Mercaptoethanol-Stable Antibody Test Predicting Hemolytic
Disease of the Newborn Due to ABO Incompatibility

Hasekura, H.

doi:10.1159/000467786

Cited by 3 publications

(5 citation statements)

References 8 publications

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“…The identification of red cell antibodies as IgM or IgG immunoglobulins often provides useful information in blood banking (Hasekura, 1974;Moor and Steane, 1976). IgM immunoglobulin may be inactivated by treatment with sulphydryl compounds such as 2-mercaptoethanol (2-ME).…”

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confidence: 99%

Evaluation of dithiothreitol (DTT) for inactivation of IgM antibodies.

Okuno¹,

Kondelis²

1978

J Clin Pathol

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The identification of red cell antibodies as IgM or IgG immunoglobulins often provides useful information in blood banking (Hasekura, 1974; Moor and Steane, 1976). IgM immunoglobulin may be inactivated by treatment with sulphydryl compounds such as 2-mercaptoethanol (2-ME). The drawbacks are its obnoxious odour and the need for dialysis of the specimen in some cases (Pirofsky and Rosner, 1974; Moor and Steane, 1976 (Webb, 1972).DTT was obtained from Sigma (St. Louis, Missouri). The procedure used was essentially the same as that described by Pirofsky and Rosner. Briefly, 0O01 M DTT is prepared in pH 7-4 phosphate buffered saline (PBS). After addition of 025 ml DTT in PBS to 025 ml of test serum, resulting in a final concentration of 0O005 M DTT, the mixture is incubated at 370C for two hours. For evaluation of the optimal conditions of DTT inactivation of IgM antibodies, changes of molarity of DTT were made by appropriately diluting the stock solution in phosphate buffered saline. The pH of the solution was altered by changing appropriately the ratio of Na2HPO and KH2PO4.The titration of antibodies was scored according to the procedure set out by the American Association of Blood Banks 1974. ResultsThe effects of incubation time, incubation temperature, and concentrations and pH of DTT were evaluated by using high-titre group 0 sera in the saline phase. The pretreatment titration of the sera ranged from 32 to 128. The effects of incubation temperature and incubation time on the reduction of titration scores by DTT are shown in Figure 1. A marked reduction was seen after a 15-minute 1152 copyright.

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Evaluation of dithiothreitol (DTT) for inactivation of IgM antibodies.

Okuno¹,

Kondelis²

1978

J Clin Pathol

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“…(a) In our study the titre of the IgG antibodies was determined after delivery, while in Hasekura's study [2] this was done in late pregnancy, and Hasekura observed that in some patients post-partum titres may become lower in relation to those found before delivery.…”

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confidence: 78%

“…Numerous techniques for the detection of IgG antibodies anti-A or -B have been proposed but those based on the neutralization of IgM antibodies by 2-mercaptoethanol seem to give the best results [1][2][3][4][5]. The method described by Reesink et al [4] is particularly attractive since it is simple and less time-consuming than other techniques, while it has proved to be as reliable as other methods.…”

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confidence: 99%

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The Titre of IgG Anti-A/B in the Serum of Group O Mothers of Incompatible Infants with Early Neonatal Jaundice

Luca¹,

Pacioni²,

Tonelli³

1976

Vox Sanguinis

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The titre of IgG anti-A/B was determined by means of a 2-mercaptoethanol test in the serum of 80 blood group O mothers, who gave birth at term to A/B incompatible infants, who developed early neonatal jaundice with a serum bilirubin level of over 10 mg/100 ml. The titres were compared with the results of the direct antiglobulin test performed on the red cells from the cord blood and the necessity for exchange transfusion. Only in mothers of group A infants, a significant correlation was found between the titre of IgG anti-A and the occurrence of a positive antiglobulin test. In no case the need for an exchange transfusion was correlated with the IgG anti-A/B titre.

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“…IgG anti-A and anti-B titers in the maternal sera were titrated in two-fold serial dilutions by IAT using 2-mercaptoethanol-treated sera accord ing to Hasekura's [10] method.…”

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confidence: 99%

IgG Subclasses of Anti-A and Anti-B Antibodies Bound to the Cord Red Cells in ABO Incompatible Pregnancies

Ukita¹,

Takahashi²,

Nunotani³

et al. 1989

Vox Sanguinis

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IgG subclasses were determined in 138 A or B infants weighing over 2,500g, born to O mothers. Direct antiglobulin test (DAT) was positive in 43 infants and negative in 95 with anti-A and/or anti-B antibodies detected by heat elution test. In 59 out of 131 infants without ABO hemolytic disease (ABO-HDN), no IgG subclass was detectable. In the 72 others, IgG1 was found in 29/72, IgG2 in 63/72, and IgG3 was not detected. In 7 infants with ABO-HDN, DAT was positive in 4 and negative in 3. In conclusion, in DAT-positive infants without HDN, IgG1 or IgG2 may be bound to erythrocytes, but the amount of IgG1 is too small to cause hemolysis. In DAT-positive ABO-HDN the amount of IgGl is sufficient to cause hemolysis. In DAT-negative ABO-HDN, IgG3 is responsible for hemolysis, even though undetectable by DAT.

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Mercaptoethanol-Stable Antibody Test Predicting Hemolytic Disease of the Newborn Due to ABO Incompatibility

Cited by 3 publications

References 8 publications

Evaluation of dithiothreitol (DTT) for inactivation of IgM antibodies.

Evaluation of dithiothreitol (DTT) for inactivation of IgM antibodies.

The Titre of IgG Anti-A/B in the Serum of Group O Mothers of Incompatible Infants with Early Neonatal Jaundice

IgG Subclasses of Anti-A and Anti-B Antibodies Bound to the Cord Red Cells in ABO Incompatible Pregnancies

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