Mensurações ultrassonográficas da cisterna da glândula mamária de caprino transgênico

Melo, Cynthia de Freitas; Sousa, Francisco Carlos de; Filho, Araújo; Moura, R.R.; Albuquerque, Erica S.; Pereira, Alexsandra Fernandes; Melo, Luciana Magalhães; Freitas, V. J. F.; Teixeira, Dárcio Ítalo Alves

doi:10.1590/s0102-09352012000200033

Cited by 7 publications

(9 citation statements)

References 5 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This typical image was a result of connective tissue with higher and gland parenchyma with lesser echogenic density. Similar ultrasonographic appearance for lactating goat mammary gland was recently described by our group (7) .…”

Section: Resultssupporting

confidence: 82%

“…B-mode ultrasonography has been used to evaluate the mammary gland in cows (10) and goats (7) . According to Franz et al (11) , ultrasonography of the udder parenchyma is a diagnostic aid in mastitis, enlargement of the udder without clinical signs of mastitis (eg.…”

Section: Resultsmentioning

confidence: 99%

“…The examination was performed in non-sedated does in standing position. The ultrasonographic technique used for mammary gland evaluation was in direct contact (7) . The transducer was placed on the caudal surface of the tested teat, in a vertical position, at an angle of approximately 30°, pointing to the gland cistern.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Mammary Biopsy in Induced Lactating Goats: Assessment of Post-Biopsy Milk Properties and Ultrasonographic Appearance

et al. 2017

View full text Add to dashboard Cite

The aim of this study was to evaluate the effects of mammary biopsy procedures on gland function of goats submitted to hormonal lactation. Ten female Caninde goats, at the age of around 3 years, were used, and lactation was induced by using estrogen, progesterone, and prednisolone. Ultrasonographic examinations of mammary gland, milking, and analysis of physicochemical properties of milk were performed immediately before (0 h) and 24, 48, and 72 h after each biopsy procedure. Surgical mammary gland biopsies were obtained at days 5 and 26 of the lactation. Even after drying initiation, all animals produced milk for five months. No abnormalities were observed on the ultrasonographic appearance of biopsied glands. All 20 biopsies were technically successful and any inflammation or infection was associated with biopsy procedures. Regarding the physicochemical properties and volumes of milk produced, no significant differences (P > 0.05) were observed between right and left glands nor in the comparison of the results from time 0 with 24, 48, and 72 h for biopsied teats. The mammary biopsy did not induce neither severe changes in production and physicochemical properties of the milk nor in ultrasonographic appearance of mammary gland of goats in hormonal lactation.Keywords: biopsy; goat; udder; ultrasonography. ResumoO objetivo deste estudo foi avaliar os efeitos dos procedimentos de biópsia mamária sobre a função da glândula de caprinos submetidos à lactação induzida. Dez cabras Canindé com idade de cerca de 3 anos foram utilizadas e a lactação foi induzida pelo uso de estrógeno, progesterona e prednisolona. Exames ultrassonográficos da glândula mamária, ordenha e análise das propriedades físico-químicas do leite foram realizados imediatamente antes (0 h) e 24, 48 e 72 h após cada procedimento de biópsia. As biópsias cirúrgicas de glândula mamária foram obtidas aos dias 5 e 26 da lactação. Mesmo após o início do processo de secagem, todos os animais continuaram produzindo leite por cinco meses. Nenhuma anormalidade foi observada na aparência ultrassonográfica das glândulas biopsiadas. Todas as 20 biópsias foram realizadas com sucesso e nenhuma inflamação ou infecção foi observada. Com relação às propriedades físico-químicas e volumes de leite produzido, nenhuma diferença significativa Cienc. anim. bras., Goiânia, v.18, 1-7, e-39077, 2017 2 SOUSA, F.C. et al.(P > 0,05) foi observada entre a glândula direita e esquerda, nem comparando resultados do momento 0 com 24, 48 e 72 h para as glândulas biopsiadas. A biópsia mamária não induz severas mudanças na produção e nas propriedades físico-químicas do leite nem na aparência ultrassonográfica da glândula mamária de caprinos em lactação.

show abstract

Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Mammary Biopsy in Induced Lactating Goats: Assessment of Post-Biopsy Milk Properties and Ultrasonographic Appearance

et al. 2017

View full text Add to dashboard Cite

show abstract

“…For evaluation of the mammary gland, ultrasonographic technique used was in direct contact (transcutaneous ultrasonography), according to Melo et al (2012). Mammary gland examinations were performed everyday immediately before milking, from the 1 st to the 17 th day of lactation.…”

Section: Ultrasonography Of Mammary Glandmentioning

confidence: 99%

Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats for hG-CSF during induced lactation

Sousa¹,

Melo

Batista

et al. 2016

SCA

View full text Add to dashboard Cite

In transgenic murine models, the study of certain organs or tissues can be performed after euthanasia of some specimens. However, this practice may not be economically feasible when applied to livestock such as transgenic goats. It is necessary to use minimally invasive methods to perform in vivo studies of organs that may be affected by disorders related to the activity of the transgene, particularly during milk production, when the recombinant protein is secreted. The aim of this study was to describe ultrasonographic findings of the liver, gallbladder, spleen, kidneys, and mammary glands in transgenic goats for evaluating the effect of human granulocyte-colony stimulating factor (hG-CSF) expression in milk during induced lactation. Six female Canindé goats-three transgenic (T) and three non-transgenic (NT)-were subjected to hormone therapy to induce lactation; ultrasonographic examinations of the liver, gallbladder, spleen, kidneys, and mammary gland were performed during both the hormonal therapy and the lactation period at different intervals depending on the organ being examined. On Day 16 (Day 1 = hormonal therapy initiation), all goats were lactating and presented healthy mammary glands, characterized by echogenic parenchyma showing a granular echotexture. Transgenic and nontransgenic goats were compared on the basis of measurements and ultrasound images obtained from each organ. No differences between T and NT animals were observed in the examined area for the liver, gallbladder, spleen, and kidneys. Liver and renal echogenicity and appearance of gallbladder and portal and hepatic veins were similar in all females. Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats did not show any difference from those in nontransgenic goats.Thus, these results suggest that the presence and expression of the transgene had no effect on the ultrasonographic findings of mammary gland and abdominal organs in the transgenic goats. Moreover, the findings shows that ultrasonography is a useful screening tool for clinical examination ResumoEm camundongos transgênicos, o estudo de certos órgãos ou tecidos pode ser viabilizado por meio da eutanásia de alguns exemplares. Entretanto, esta prática pode não ser economicamente viável quando aplicada a animais de produção, como caprinos transgênicos. É necessário utilizar métodos minimamente invasivos para estudar in vivo os órgãos que podem estar envolvidos em desordens relacionadas à atividade do transgene, principalmente durante a produção de leite, quando a proteína recombinante é secretada. O objetivo deste estudo foi descrever as características ultrassonográficas de fígado, vesícula biliar, baço, rins e glândula mamária de caprinos transgênicos expressando o fator estimulante de colônias de granulócitos humano (hG-CSF) no leite, durante lactação induzida. Seis fêmeas caprinas da raça Canindé: três não transgênicas (NT) e três transgênicas (T) foram submetidas a tratamento hormonal para indução da lactação; os exa...

show abstract

“…Ces dernières années, l'échographie a été appliquée pour examiner la structure et la pathologie mammaires chez les vaches in vivo (Buczinski et Descôteaux, 2009;Esselburn, 2012). Chez la chèvre laitière, des résultats importants ont été rapportés sur les flux de lait dans les citernes au moyen de sondes échographiques (Wojtowski et al, 2006;Fasulkov et al, 2010;Melo et al, 2012). L'observation par échographie de l'organisation interne et du flux de lait dans les citernes mammaires est d'utilité zootechnique importante en clinique, en production animale ou en recherche appliquée (Buczinski et Descôteaux, 2009;Fasulkov, 2012).…”

Section: Introductionunclassified

Imagerie échographique de la glande mammaire de la chèvre en lactation

Haro¹,

Zongo²,

Bazie³

et al. 2017

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

RESUME L'échographie est appliquée à la mamelle. L'objectif est d'apprécier l'anatomie interne et le flux de lait des citernes mammaires pendant la lactation chez la chèvre sahélienne. L'étude a porté sur 5 chèvres multipares, d'âge compris entre [36][37][38][39][40][41][42][43][44][45][46][47][48] mois et de poids moyen de 32,5±7,3 kilogrammes. Les examens ont été réalisés une fois par semaine pendant cinq semaines. Ils sont pratiqués sur des femelles séparées de leurs petits pendant au moins 12 heures. Le remplissage des citernes a été réalisé à 2, 4, 6 et 8 heures après la tétée. Sur la mamelle rasée, la sonde a été appliquée longitudinalement à l'angle du trayon. Le volume moyen de lait après 12 heures a été de 59,13±37,63 millilitres. Après la tétée le volume moyen de lait résiduel a été de 6,72±4,16 millilitres. Le remplissage des citernes au fil du temps après la tétée a été de 9,33±5,28, 15,01±8,10, 22,74±10,54, et 36,04±20,41 millilitres respectivement. La vitesse moyenne de remplissage des citernes mammaires a été de 3,66±2,25 millilitre /heure. La vitesse de remplissage a été plus élevée (5,26±1,97 millilitre /heure) pendant les quatre dernières heures. En somme, l'échographie a permis d'apprécier les flux de lait des citernes mammaires de la chèvre sahélienne en lactation. © 2017 International Formulae Group. All rights reserved.Mots clés: Chèvre sahélienne, échographie, citerne mammaire. Ultrasonographic imagery of mammary gland of the goat in lactation ABSTRACTUltrasonography is applied to the udder. The objective is to appreciate the internal anatomy and the milk flow of the mammary cisterns during lactation in the sahelian goat. The study related to 5 goats multipares, of age ranging between [36-48] month and of average weight of 32.5±7.3 kilogrammes. The tests were carried out once per week during five weeks. They were carried out on separate females the small ones during at least 12 hours. The filling of the cisterns was carried out at 2, 4, 6 and 8 hours after suckling. To the shaven udder, the probe was applied longitudinally to the angle of the teat. The average volume of milk after 12 hours was of 59.13±37.63 millilitres. After suckling the average volume of residual milk was of 6.72±4.16 millilitres. The filling of the cisterns to the wire of time after suckling was of 9.33±5.28, 15.01±8.10, 22.74±10.54, and 36.04±20.41 millilitres respectively. The mean velocity of filling of the mammary cisterns was of 3.66±2.25 millilitre / hour. The speed of filling was higher (5.26±1.97 millilitre / hour) during the four M. HARO et al. / Int. J. Biol. Chem. Sci. 11(3): 1307-1314, 2017 1308 last hours. All things considered, ultrasonography made it possible to appreciate milk flows of the mammary cisterns of the sahelian goat in lactation.

show abstract

Mensurações ultrassonográficas da cisterna da glândula mamária de caprino transgênico

Abstract: Comunicação [Communication]Mensurações ultrassonográficas da cisterna da glândula mamária de caprino transgênico [

Cited by 7 publications

References 5 publications

Mammary Biopsy in Induced Lactating Goats: Assessment of Post-Biopsy Milk Properties and Ultrasonographic Appearance

Mammary Biopsy in Induced Lactating Goats: Assessment of Post-Biopsy Milk Properties and Ultrasonographic Appearance

Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats for hG-CSF during induced lactation

Imagerie échographique de la glande mammaire de la chèvre en lactation

Contact Info

Product

Resources

About