Menstrual blood-derived mesenchymal stem cells differentiate into functional hepatocyte-like cells

Mou, Xiaozhou; Lin, Jian; Chen, Jin-yang; Li, Yifei; Wu, Xiaoxing; Xiang, Bingyu; Li, Caiyun; Ma, Juming; Xiang, Charlie

doi:10.1631/jzus.b1300081

Cited by 56 publications

(63 citation statements)

References 43 publications

(74 reference statements)

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“…However, some groups have reported positive expression of embryonic markers such as SSEA-4 and Nanog in MenSCs that were not found on MSCs from other sources (7–10). This raises the question whether these cells presenting earlier markers of stemness represent a more primitive progenitor than MSCs from other sources.…”

Section: Are Menscs Just Another Mscs Source?mentioning

confidence: 96%

The Promising Potential of Menstrual Stem Cells for Antenatal Diagnosis and Cell Therapy

et al. 2014

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Menstrual-derived stem cells (MenSCs) are a new source of mesenchymal stem cells isolated from the menstrual fluid. Currently, there is a growing interest in their clinical potential due to fact that they are multipotent, highly proliferative, and easy to obtain in a non-invasive manner. Sampling can be repeated periodically in a simplified and reproducible manner devoid of complications that no existing cell source can match. MenSCs are also free of ethical dilemmas, and display novel properties with regard to presently known adult derived stem cells. This review details their distinctive biological properties regarding immunophenotype and function, proliferation rate, differentiation potential, and paracrine effects mediated by secreted factors. Their possible role in antenatal diagnosis is also discussed. While more insight on their immunomodulatory and diagnostic properties is needed, the impact of clinical and epidemiological factors, such as age, use of contraceptives, or hormonal status still requires further investigations to properly assess their current and future use in clinical application and diagnosis.

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Section: Are Menscs Just Another Mscs Source?mentioning

confidence: 96%

The Promising Potential of Menstrual Stem Cells for Antenatal Diagnosis and Cell Therapy

et al. 2014

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“…MenSCs were isolated and provided by the State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University (Mou et al 2013, Wu et al 2014, Lai et al 2015. Briefly, human menstrual blood was collected The χ 2 test was applied.…”

Section: Menscs Culture and Labelingmentioning

confidence: 99%

Endometrial stem cells repair injured endometrium and induce angiogenesis via AKT and ERK pathways

et al. 2016

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Intrauterine adhesions are common acquired endometrial syndromes secondary to endometrial injury, with limited effective therapies. Recently, several studies have reported that bone marrow stem cells (BMSCs) could repair injured endometrium in animal experiments. However, the role of stem cells in endometrial injury repair and its therapeutic mechanisms remain unclear. Here, we established mouse endometrial injury model and examined the benefit of human endometrial mesenchymal stem cells derived from menstrual blood (MenSCs) in restoration of injured endometrium. Injured endometrium exhibited significantly accelerated restoration at Day 7 after MenSCs transplantation, with increased endometrial thickness and microvessel density. Moreover, the fertility of mice with injured endometrium was improved, with higher conception rate (53.57% vs 14.29%, P = 0.014) and larger embryo number (3.1 ± 0.6 vs 0.9 ± 0.7, P = 0.030) in MenSCs group than control group, while no difference was found in undamaged horns between two groups. Conditioned medium from MenSCs (MenSCs-CM) could decrease H 2 O 2 -induced apoptosis of human umbilical vein endothelial cells (HUVECs) and promote proliferation, migration and angiogenesis. Angiogenesis effect of MenSCs-CM was also confirmed in Matrigel plug assay in mice. Furthermore, we discovered that MenSCs-CM could activate AKT and ERK pathways and induce the overexpression of eNOS, VEGFA, VEGFR1, VEGFR2 and TIE2 in HUVECs, which are critical in MenSCs-CM-induced angiogenesis. Angiogenesis induced by MenSCs-CM could be reversed by inhibitors of AKT and/or ERK. Taken together, we concluded that MenSCs could restore injured endometrium and improve the fertility of the endometrial injury mice, which was partially attributed to angiogenesis induced by MenSCs.Reproduction (2016) 152 389-402

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“…EnSCs can be easily isolated from menstrual blood by noninvasive acquisition. The noncontroversial extraction is also autologous and thus avoiding critical ethical issues9. A large number of reports have shown the therapeutic potential of EnSCs in restoring tissue function, such as treating myocardial infarction10, ameliorating stroke11, and improving chemotherapy-induced premature ovarian failure/insufficiency12.…”

mentioning

confidence: 99%

Human endometrial mesenchymal stem cells exhibit intrinsic anti-tumor properties on human epithelial ovarian cancer cells

Wang

Zhang

et al. 2016

Sci Rep

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Epithelial ovarian cancer (EOC) is the most lethal tumor of all gynecologic tumors. There is no curative therapy for EOC thus far. The tumor-homing ability of adult mesenchymal stem cells (MSCs) provide the promising potential to use them as vehicles to transport therapeutic agents to the site of tumor. Meanwhile, studies have showed the intrinsic anti-tumor properties of MSCs against various kinds of cancer, including epithelial ovarian cancer. Human endometrial mesenchymal stem cells (EnSCs) derived from menstrual blood are a novel source for adult MSCs and exert restorative function in some diseases. Whether EnSCs endow innate anti-tumor properties on EOC cells has never been reported. By using tumor-bearing animal model and ex vivo experiments, we found that EnSCs attenuated tumor growth by inducing cell cycle arrest, promoting apoptosis, disturbing mitochondria membrane potential and decreasing pro-angiogenic ability in EOC cells in vitro and/or in vivo. Furthermore, EnSCs decreased AKT phosphorylation and promoted nuclear translocation of Forkhead box O-3a (FoxO3a) in EOC cells. Collectively, our findings elucidated the potential intrinsic anti-tumor properties of EnSCs on EOC cells in vivo and in vitro. This research provides a potential strategy for EnSC-based anti-cancer therapy against epithelial ovarian cancer.

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Menstrual blood-derived mesenchymal stem cells differentiate into functional hepatocyte-like cells

Cited by 56 publications

References 43 publications

The Promising Potential of Menstrual Stem Cells for Antenatal Diagnosis and Cell Therapy

The Promising Potential of Menstrual Stem Cells for Antenatal Diagnosis and Cell Therapy

Endometrial stem cells repair injured endometrium and induce angiogenesis via AKT and ERK pathways

Human endometrial mesenchymal stem cells exhibit intrinsic anti-tumor properties on human epithelial ovarian cancer cells

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