Meniscus Transplantation Using Treated Xenogeneic Meniscal Tissue: Viability and Chondroprotection Study in Rabbits

Jiang, Dong; Zhao, Lianming; Tian, Ming; Zhang, Jiying; Yu, Jia-Kuo

doi:10.1016/j.arthro.2012.01.001

Cited by 33 publications

(28 citation statements)

References 37 publications

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“…Transplantation also faces numerous challenges, including integrating the allograft with host tissue, obtaining limited donor tissue, preserving and processing donor tissue, achieving a match between donor and host meniscal size, fixing the grafted tissue, and avoiding infection and immune rejection [5]. The recently described procedure of meniscal xenogeneic transplantation shows promise for avoiding some of these problems, but much work is needed before it can be applied in the clinic [6]. …”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization, and Multipotent Differentiation of Mesenchymal Stem Cells Derived from Meniscal Debris

Xie

et al. 2016

Stem Cells International

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This study aimed to culture and characterize mesenchymal stem cells derived from meniscal debris. Cells in meniscal debris from patients with meniscal injury were isolated by enzymatic digestion, cultured in vitro to the third passage, and analyzed by light microscopy to observe morphology and growth. Third-passage cultures were also analyzed for immunophenotype and ability to differentiate into osteogenic, adipogenic, and chondrogenic lineages. After 4-5 days in culture, cells showed a long fusiform shape and adhered to the plastic walls. After 10–12 days, cell clusters and colonies were observed. Third-passage cells showed uniform morphology and good proliferation. They expressed CD44, CD90, and CD105 but were negative for CD34 and CD45. Cultures induced to differentiate via osteogenesis became positive for Alizarin Red staining as well as alkaline phosphatase activity. Cultures induced to undergo adipogenesis were positive for Oil Red O staining. Cultures induced to undergo chondrogenesis were positive for staining with Toluidine Blue, Alcian Blue, and type II collagen immunohistochemistry, indicating cartilage-specific matrix. These results indicate that the cells we cultured from meniscal debris are mesenchymal stem cells capable of differentiating along three lineages. These stem cells may be valuable source for meniscal regeneration.

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Section: Introductionmentioning

confidence: 99%

Isolation, Characterization, and Multipotent Differentiation of Mesenchymal Stem Cells Derived from Meniscal Debris

Xie

et al. 2016

Stem Cells International

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“…By week twenty-four, tissue integration increased and foreign body response was not statistically significant between the groups. Both allograft and xenograft groups exhibited degeneration of the AC, but considerably decreased the rate of AC degeneration [60] .…”

Section: Permanent Scaffoldsmentioning

confidence: 92%

“…An unrelated study by Jiang et al tested whether a treated xenogeneic scaffold could adequately serve as a total meniscus replacement in an animal model [60] . The study obtained the medial menisci of 24 porcine (xenograft) and 24 rabbits (allograft), irradiated the menisci with gamma irradiation, and froze the meniscus at -80˚C for six to fourteen days.…”

Section: Permanent Scaffoldsmentioning

confidence: 99%

Tissue engineering of the meniscus: Scaffolds for meniscus repair and replacement

2015

Musculoskelet Regen

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The menisci homogenously distribute load throughout the knee joint. Partial or total removal of the menisci significantly alters the biomechanical properties of the knee, leading to osteoarthritis and articular cartilage degeneration. Many synthetic and natural materials have been tested to find a scaffold that can withstand the natural mechanical forces on the joint. However, currently there are few meniscus tissue replacements available for clinical use. This review addresses tissue engineering of the meniscus, with a primary focus on tissue-engineered scaffolds as current and future models for meniscus replacement. Our review also discusses implant fixation, an area which we believe must be improved in order for meniscal replacements to be successful in vivo.

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“…Ideal candidates for MAT are young to middle-aged patients presenting with moderate to severe pain post-meniscectomy11). There are several types of allografts available when performing an MAT, including fresh-frozen meniscal allografts with or without bone plugs, non-irradiated or irradiated12,13). Despite its efficacy, a recent study has encouraged the exploration of new preservation techniques aimed at decreasing the apoptosis-mediated cell loss that occurs in cryopreservation14).…”

Section: Introductionmentioning

confidence: 99%

Meniscal Transplants and Scaffolds: A Systematic Review of the Literature

Dangelmajer

Familiari

et al. 2017

Knee Surg Relat Res

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The reported incidence of meniscal tears is approximately 61 per 100,000. In instances where preservation of the native meniscus is no longer a feasible option, meniscal allograft transplantation (MAT) and implants or scaffolds may be considered. The goal of this review was to compare the success and failure rates of two techniques, MAT and meniscal scaffolds, and make an inference which treatment is more preferable at the present time and future. Studies that met inclusion criteria were assessed for technique used, type of transplant used, number of procedures included in the study, mean age of patients, mean follow-up time, number of failures, failure rate, and reported reoperation rate. Fifteen studies for the MAT group and 7 studies for the meniscal scaffold group were identified. In this selection of studies, the average failure rate in the MAT group was 18.7% and average reoperation rate was 31.3%. The average failure rate in the meniscal scaffold group was 5.6%, and average reoperation rate was 6.9%. It appears that although MAT is associated with high reoperation and failure rates, the limited number of studies on both MAT and scaffolds and mainly short-term results of scaffold studies make it difficult to make an objective comparison.

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Meniscus Transplantation Using Treated Xenogeneic Meniscal Tissue: Viability and Chondroprotection Study in Rabbits

Cited by 33 publications

References 37 publications

Isolation, Characterization, and Multipotent Differentiation of Mesenchymal Stem Cells Derived from Meniscal Debris

Isolation, Characterization, and Multipotent Differentiation of Mesenchymal Stem Cells Derived from Meniscal Debris

Tissue engineering of the meniscus: Scaffolds for meniscus repair and replacement

Meniscal Transplants and Scaffolds: A Systematic Review of the Literature

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