MEMS based fiber optical microendoscopes

Qiu, Zhen; Piyawattanametha, Wibool

doi:10.1016/j.displa.2014.12.001

Cited by 9 publications

(6 citation statements)

References 73 publications

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“…Although several axial-scanning solutions have been demonstrated for FCMs, these have not been sucessfully translated to in vivo neuronal recording in freely-moving animals due to various drawbacks in these methods. For example, MEMS mirrors can be modified to include an axial scanner, but this adds mechanical and optical complexity that limit miniaturizion 16 . Axial-scanning can also be accomplished using an electrically tunable lens (ETL) to adjust the focal length (FL) of the imaging system.…”

Section: Introductionmentioning

confidence: 99%

Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning

Ozbay

Futia

et al. 2018

Sci Rep

110

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We present a miniature head mounted two-photon fiber-coupled microscope (2P-FCM) for neuronal imaging with active axial focusing enabled using a miniature electrowetting lens. We show three-dimensional two-photon imaging of neuronal structure and record neuronal activity from GCaMP6s fluorescence from multiple focal planes in a freely-moving mouse. Two-color simultaneous imaging of GFP and tdTomato fluorescence is also demonstrated. Additionally, dynamic control of the axial scanning of the electrowetting lens allows tilting of the focal plane enabling neurons in multiple depths to be imaged in a single plane. Two-photon imaging allows increased penetration depth in tissue yielding a working distance of 450 μm with an additional 180 μm of active axial focusing. The objective NA is 0.45 with a lateral resolution of 1.8 μm, an axial resolution of 10 μm, and a field-of-view of 240 μm diameter. The 2P-FCM has a weight of only ~2.5 g and is capable of repeatable and stable head-attachment. The 2P-FCM with dynamic axial scanning provides a new capability to record from functionally distinct neuronal layers, opening new opportunities in neuroscience research.

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Section: Introductionmentioning

confidence: 99%

Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning

Ozbay

Futia

et al. 2018

Sci Rep

110

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“…A variety of scanning mechanisms have been employed in distal scanning CLEs, including piezoelectric fiber scanners [10], electromagnetically actuated resonant armature systems [7], and various microelectromechanical system (MEMS) mirrors [11], [12], but scanning MEMS mirrors are drawing the most attention. In past decades, electrostatic [13], [14], [15], [16], piezoelectric [17], [18], electromagnetic [19], and electrothermal [20], [21] MEMS mirrors have been applied to miniaturize confocal microscopes.…”

Section: Introductionmentioning

confidence: 99%

A MEMS Mirror-Based Confocal Laser Endomicroscope with Image Distortion Correction

et al. 2023

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Scanning MEMS mirrors can extend confocal laser microscopy into endoscopic applications, but the practical use of MEMS mirror-based confocal endomicroscopy is hindered partially by various image distortions such as barrel, fan-shaped and nonlinear distortions. In this work, the nonlinear scanning behaviors of an electrothermal MEMS mirror are analyzed and incorporated into an optical scanning model that takes all these three types of distortions into account. The model generates a 2D spatial mapping that can be applied to correct all of the image distortions in one step without a calibration board. To experimentally validate this method, a confocal laser endomicroscope employing a two-axis scanning electrothermal MEMS micromirror is designed and constructed, and confocal fluorescence images of a patterned micro-structure are obtained with the MEMS endomicroscope. The results show that the overall image distortion is reduced by at least one order of magnitude in the length direction.

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“…In the case of, e.g., spectral-domain OCT [4,5], the axial dimension of the probed volume is obtained through spectrally-resolved measurements, whereas the other two result from a 2D scan, hence justifying the conspicuous need for 2D scanners compatible with endoscopic requirements. Among them, endoscopic apparatuses face increasing challenges in reaching more and more remote and restricted areas (colonoscopy, esophagus, veins) [6], requiring drastic means to downsize the systems. In order to meet the specifications of miniaturization, MEMS are particularly appreciated for their compact design, simplicity of fabrication, integrability and low-cost [7,8].…”

Section: Introductionmentioning

confidence: 99%

Real-time Lissajous imaging with a low-voltage 2-axis MEMS scanner based on electrothermal actuation

Tanguy¹,

Gaiffe²,

Passilly³

et al. 2020

Opt. Express

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Laser scanning based on Micro-Electro-Mechanical Systems (MEMS) scanners has become very attractive for biomedical endoscopic imaging, such as confocal microscopy or Optical Coherence Tomography (OCT). These scanners are required to be fast to achieve real-time image reconstruction while working at low actuation voltage to comply with medical standards. In this context, we report a 2-axis Micro-Electro-Mechanical Systems (MEMS) electrothermal micro-scannercapable of imaging large fields of view at high frame rates, e.g. from 10 to 80 frames per second. For this purpose, Lissajous scan parameters are chosen to provide the optimal image quality within the scanner capabilities and the sampling rate limit, resulting from the limited A-scan rate of typical swept-sources used for OCT. Images of 233 px × 203 px and 53 px × 53 px at 10 fps and 61 fps, respectively, are experimentally obtained and demonstrate the potential of this micro-scannerfor high definition and high frame rate endoscopic Lissajous imaging.

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MEMS based fiber optical microendoscopes

Cited by 9 publications

References 73 publications

Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning

Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning

A MEMS Mirror-Based Confocal Laser Endomicroscope with Image Distortion Correction

Real-time Lissajous imaging with a low-voltage 2-axis MEMS scanner based on electrothermal actuation

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