Memory deficits, gait ataxia and neuronal loss in the hippocampus and cerebellum in mice that are heterozygous for Pur-alpha

Abstract

Pur-alpha is a highly conserved sequence specific DNA and RNA binding protein with established roles in DNA replication, RNA translation, cell cycle regulation, and maintenance of neuronal differentiation. Prior studies have shown that mice lacking Pur-alpha (−/−) display decreased neurogenesis and impaired neuronal differentiation. We sought to examine for the first time, the behavioral phenotype and brain histopathology of mice that are heterozygous (+/−) for Pur-alpha. Standardized behavioral phenotyping revealed a decreased escape response to touch, limb and abdominal hypotonia, and gait abnormalities in heterozygous Pur-alpha (+/−) mice, compared to wild-type (+/+) littermates. Footprint pattern analyses showed wider-based steps, increased missteps and more outwardly rotated hindpaws in heterozygous Pur-alpha (+/−) mice, suggestive of cerebellar pathology. Barnes maze and novel object location testing revealed significant memory deficits in heterozygous Pur-alpha mice, suggestive of hippocampal pathology. Quantitative immunohistochemical assays of the vermal region of the cerebellum and CA1-3 regions of the hippocampus revealed reduced numbers of neurons in general, as well as reduced numbers of Pur-alpha+-immunopositive neurons and dendrites in heterozygous Pur-alpha mice, compared to wild type littermates. Past studies have implicated mutations in Pur-alpha in several diseases of brain development and neurodegeneration. When combined with these new findings, the Pur-alpha heterozygous knockout mice may provide an animal model in which to study mechanisms of and treatments for Pur-alpha related cognitive deficiencies and neuropathology.

PURA syndrome: clinical delineation and genotype-phenotype study in 32 individuals with review of published literature

“…Immunohistochemical assays displayed a reduced number of neurons in cerebellum and hippocampus of these animals, which is in line with their neurological, behavioural and cognitive phenotypes. 17 …”

Patient with a novel purine‐rich element binding protein A mutation

“…The development of dendrites is also abnormal in the cerebellum and hippocampus of Pura ‐/‐ mice (Khalili et al ). Recently, Barbe et al () showed that mice that are heterozygous for Pur‐alpha show memory deficits, gait ataxia and neuronal loss in the hippocampus and cerebellum.…”

Lumbar to sacral root rerouting to restore bladder function in a feline spinal cord injury model: Urodynamic and retrograde nerve tracing results from a pilot study

“…Sequential sections were mounted onto charged slides. Every third section was analyzed for presence of Fluorogold labeled neurons in ventral horns of spinal cord segments of L6‐S2, as previously described . The mean and standard error of estimated number of Fluorogold labeled neurons per volume (mm 3 ) of spinal cord segment is reported.…”

“…Every third section was analyzed for presence of Fluorogold labeled neurons in ventral horns of spinal cord segments of L6-S2, as previously described. 22 The mean and standard error of estimated number of Fluorogold labeled neurons per volume (mm 3 ) of spinal cord segment is reported. Adjacent sections were stained with hematoxylin and eosin (H&E) and examined for integrity of the spinal cord and anastomosis site using a Nikon bright-field microscope FIGURE 1 Design and a rerouted root example.…”