Membranotropic effect of 2(chloroethoxy)-para-N-dimethylaminophenyl phosphinylacetyl hydrazide (CAPAH)

Abstract

Membranostabilizing effect of CAPAH is demonstrated in vivo and in vitro. The preparation decreases experimental osmotic hemolysis and prevents elevation of blood enzymes in rats exposed to hypoxia. Infrared spectroscopy and electron paramagnetic resonance show that CAPAH is located in the superticial hydrophilic layer of modeled phosphatidylcholine membranes and interacts with lipid molecules.

31P NMR and FTIR studies on the interaction of phosphorylacetohydrazides with dipalmitoyl phosphatidylcholine model membranes

“…At present the phosphorylacetohydrazides we selected for this research are the only synthetic nootropic preparations having a phosphorylic group. Nevertheless, they render the same effect on the structural organization of the PC membranes [19][20][21]. In general, the results obtained by FTIR and 3lp NMR spectroscopies may be interpreted on the basis of the following model of the interaction of phosenazid and its hydrochloride salt with lipid membranes: the drug molecules penetrate the bilayer down to the glycerol backbone.…”

“…As a result, the membrane becomes more fluid. The symmetric disordering of both hydrophilic and hydrophobic regions of the membrane under the influence of phosphorylacetohydrazides is probably the cause of their membrane-stabilizing action revealed on the model of osmotic hemolysis of rat erythrocytes [19]. It is assumed [2,4] that new phospholipid-nootropic drug organization confers a better cell membrane viscosity leading to a better cell resistance to extemal aggression and promoting the processes of recording and keeping information in synapse membranes.…”