Membranes as Targets for Hyperthermic Cell Killing

Konings, Antonius W.T.

doi:10.1007/978-3-642-83263-5_2

Cited by 36 publications

(17 citation statements)

References 34 publications

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“…Recently we demonstrated in the same model that the increase in platinum concentration in normal tissues after the combined treatment remained behind of that in tumours. Except in the kidney, in the case of cDDP treatment combined with hyperthermia, no other severe increase in tissue platinum concentration resulting in toxicity could be observed (Los et al, 1991b(Los et al, , 1992 (Konings et al, 1988). Increasing the temperature may result in an increased permeability of the cell membrane (Arancia, 1989 For i.p.…”

Section: Discussionmentioning

confidence: 99%

Response of peritoneal solid tumours after intraperitoneal chemohyperthermia treatment with cisplatin or carboplatin

Los

Vugt²,

Pinedo³

1994

Br J Cancer

128

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The combination of heat and chemotherapy was studied in an intraperitoneal tumour model. Rats bearing peritoneal CC531 tumours (2-6 mm) were treated i.p. with cDDP or CBDCA [maximal tolerated dose (MTD)] in combination with regional hyperthermia (41.5 degrees C, 1 h) of the peritoneal cavity. The addition of hyperthermia to the i.p. treatment led to a decrease in the MTD of cDDP by 33.3% at 41.5 degrees C. This was due to increased nephrotoxicity. The MTD of CBDCA did not change as a result of hyperthermia treatment. The chemo-hyperthermia treatment resulted in more cDDP or CBDCA DNA adducts in peritoneal tumours after the combined treatment than after chemotherapy alone. The increased tumour platinum concentrations, rising from 1.3 micrograms Pt g-1 tumour at 37 degrees C to 5.4 micrograms Pt g-1 tumour at 41.5 degrees C for cDDP and from 0.2 microgram Pt g-1 tumor to 0.7 microgram Pt g-1 tumour at 41.5 degrees C for CBDCA, contributed considerably to the enhanced numbers of cDDP or CBDCA DNA adducts. As a result of the latter, i.p. chemotherapy combined with regional hyperthermia led to an increase in tumour growth delay (TGD) after increasing the temperature to 41.5 degrees C for cDDP and CBDCA (by 40 days for cDDP, 22 days for CBDCA). These data were in agreement with the in vitro findings, i.e. that higher temperatures led to increased cytotoxicity.

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Section: Discussionmentioning

confidence: 99%

Response of peritoneal solid tumours after intraperitoneal chemohyperthermia treatment with cisplatin or carboplatin

Los

Vugt²,

Pinedo³

1994

Br J Cancer

128

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“…It is known that elevated temperatures in crease the mobility and hence the fluidity of membrane molecules, leading to an increase in permeability [16,17]. The cellular uptake of various anticancer drugs with hyperthermia has been reported [18].…”

Section: Discussionmentioning

confidence: 99%

In vitro Effect of Hyperthermia on Chemoenhancement and Uptake of Cisplatin in Human Pharyngeal Carcinoma KB Cells

Ohtsubo

Saitô²,

Tanaka³

et al. 1997

Chemotherapy

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The purpose of this study was to assess the efficacy of hyperthermia (42 or 44 °C) as a modifier of cis-diamminedichloro-platinum (II) (CDDP) cytotoxicity and platinum incorporation in human pharyngeal carcinoma KB cells. To maximize the interactive effect, we examined the time sequence of high (above 43 °C) or low (below 43 °C) hyperthermia and CDDP. Within the dose range of CDDP studied, there was a marked synergism between the effects of heating at 44 °C and subsequent CDDP exposure for 5 h. Pretreatment at 44 °C for 30 min or at 42 ° C for 4 h enhanced CDDP cytotoxicity more than posttreatment at 44 °C for 30 min or at 42 °C for 4h. However, the chemoenhancement ratio of pretreatment at 44 ° C for 30 min was higher than that of pretreatment at 42 ° C for 4 h, although the thermal isotoxic dose decreased the cell count to 60% under both conditions. There was a significant increase in CDDP uptake after hyperthermia at 44 °C. These results indicate that high hyperthermia effectively enhances subsequent CDDP cytotoxicity in human pharyngeal carcinoma KB cells.

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“…Lowdose hyperthermia damage is generally considered to cause protein damage in a wide range of subcellular sites, such as the membrane (Konings, 1988), cytoskeleton (Welch and Suhan, 1985), mitochondria (Dickson and Calderwood, 1979) or nucleus (Welch and' Suhan, 1985), in addition to inhibiting protein synthesis in a dose-dependent manner (Black and Subjeck, 1989). Higher levels of heat treatments (45-98°C) induce DNA damage, such as DNA 'melting' (Lepock and Kruuv, 1992).…”

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confidence: 99%

A comet assay of DNA damage and repair in K562 cells after photodynamic therapy using haematoporphyrin derivative, methylene blue and meso-tetrahydroxyphenylchlorin

McNair

Marples²,

West³

et al. 1997

Br J Cancer

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Summary Single-cell electrophoresis (comet assay) has been used to evaluate DNA damage and repair in the human myeloid leukaemia cell line K562 after low-dose (predominantly sub-lethal) treatments of hyperthermia and photodynamic therapy (PDT). Three different photosensitizers were examined: haematoporphyrin derivative (HpD), methylene blue (MB) and meso-tetrahydroxyphenylchlorin (mTHPC). None of the drugs in the absence of light, nor in light alone, resulted in detectable DNA damage. However, a significant amount of DNA damage was detected immediately after treatment with haematoporphyrin derivative or methylene blue PDT compared with drug-only or lightonly treatments; no residual level of DNA damage was evident for either drug following a 4-h post-treatment incubation at 370C. No significant DNA damage was detected after meso-tetrahydroxyphenylchlorin PDT or hyperthermia either immediately or 4 h after treatment. We conclude that the alkaline comet assay can be applied as an effective screening assay for DNA damage induced by a range of laser therapies.

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Membranes as Targets for Hyperthermic Cell Killing

Cited by 36 publications

References 34 publications

Response of peritoneal solid tumours after intraperitoneal chemohyperthermia treatment with cisplatin or carboplatin

Response of peritoneal solid tumours after intraperitoneal chemohyperthermia treatment with cisplatin or carboplatin

In vitro Effect of Hyperthermia on Chemoenhancement and Uptake of Cisplatin in Human Pharyngeal Carcinoma KB Cells

A comet assay of DNA damage and repair in K562 cells after photodynamic therapy using haematoporphyrin derivative, methylene blue and meso-tetrahydroxyphenylchlorin

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