Membrane-type 1 matrix metalloproteinase mRNA expression in colorectal cancer

Abstract

Preliminary results indicate that membrane-type 1 matrix metalloproteinase levels correlate with increasing tumor stage.

Effects of Estrogen on Messenger Ribonucleic Acid Expression Patterns of Extracellular Matrix Proteases in the Primate Colon

“…MMP-14 mRNA expres- sion has been localized to colon carcinoma tissue, and expression progressively increases with the transition from normal through adenoma to carcinoma. 19,26,43 In the present study, there was a trend toward decreased expression of MMP-14 with estrogen replacement in primate colon tissue; however, no statistical difference existed between groups (Fig. 3).…”

“…[16][17][18][19] Additionally, estrogen may increase expression of certain protease inhibitors, such as tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) or TIMP-2. We used a nonhuman primate model of in vivo estrogen ablation and replacement: oophorectomized female rhesus monkeys untreated (estrogen-deprived) or treated with physiologic concentrations of replacement estradiol for five months.…”

Increased Membrane Type 1 Matrix Metalloproteinase Expression from Adenoma to Colon Cancer

“…[9][10][11]13,[19][20][21][22][23][24][25][26] A correlation of increased MT1-MMP mRNA with advanced tumor stage has been demonstrated in colon cancer. 27 However, little is known about the expression of this enzyme in preinvasive colon lesions or whether there is any variation in MT1-MMP expression in node-negative (TxN0) colon cancer patients who have experienced recurrence within five years compared with those who have not. Because there is a clear association between MT1-MMP overexpression and increased invasiveness of tumor cells in experimental in vitro models, the purpose of the present study was to determine whether there is a progressive increase in the expression of MT1-MMP in humans when normal mucosal tissue is compared with adenoma, carcinoma in situ, and invasive cancer.…”

Tissue levels of active matrix metalloproteinase-2 and -9 in colorectal cancer

“…In colorectal cancer (CRC) most of the data are on mRNA, which is upregulated in tumour cells and/or tumour stroma, if compared to normal healthy tissue. For instance, upregulation of mRNA has been reported for MMP-2 (Pyke et al, 1993), MMP-7 (Mori et al, 1995), MMP-9 , MMP-11 (Thewes et al, 1996), MMP-12 (Yang et al, 2001) and MMP-14 (Sardinha et al, 2000). In the immunohistochemical detection of MMPs the focus has been on the analysis of MMP localisation.…”