Membrane type 1 matrix metalloproteinase is a crucial promoter of synovial invasion in human rheumatoid arthritis

Membrane-Type I Matrix Metalloproteinase-Dependent Regulation of Rheumatoid Arthritis Synoviocyte Function

“…Corroborating our findings, they also found that the tissue-invasive properties of isolated RA synoviocytes could be blocked by TlMP-2 but not TlMP-1 (53). Although the specific roles of secreted collagenases and MT-MMPs were not evaluated directly in their work, RA synoviocyte invasion was inhibited when cells were infected with an adenoviral vector encoding a MT1-MMP dominant-negative fragment previously reported to interfere with the catalytic activity of the wild-type enzyme (53). At this juncture, however, the utility of this indirect approach for implicating MT1-MMP can only be embraced cautiously because given that others have reported that the MT1-MMP fragment enhances, rather than inhibits, MT1-MMP proteolytic activity (54).…”

“…While our work was in preparation, Miller et al (53) reported that MT1-MMP is expressed in human RA pannus tissue, particularly within synoviocyte populations localized to the pannus-cartilage junction. Corroborating our findings, they also found that the tissue-invasive properties of isolated RA synoviocytes could be blocked by TlMP-2 but not TlMP-1 (53).…”

“…While our work was in preparation, Miller et al (53) reported that MT1-MMP is expressed in human RA pannus tissue, particularly within synoviocyte populations localized to the pannus-cartilage junction. Corroborating our findings, they also found that the tissue-invasive properties of isolated RA synoviocytes could be blocked by TlMP-2 but not TlMP-1 (53). Although the specific roles of secreted collagenases and MT-MMPs were not evaluated directly in their work, RA synoviocyte invasion was inhibited when cells were infected with an adenoviral vector encoding a MT1-MMP dominant-negative fragment previously reported to interfere with the catalytic activity of the wild-type enzyme (53).…”

Phosphorylation and mRNA Splicing of Collapsin Response Mediator Protein-2 Determine Inhibition of Rho-associated Protein Kinase (ROCK) II Function in Carcinoma Cell Migration and Invasion

“…Cell matrix type I-A (Nitta gelatin; Osaka, Japan) was prepared as described previously (37), and inserts were coated by the addition of 200 l of matrix solution and removal of 150 l, followed by 30-min incubation at 37°C in cell incubator prior to the assay. Serumstarved transfected SW620 cells were prepared as described previously (21).…”

Matrix metalloproteinase 13 is a new contributor to skeletal muscle regeneration and critical for myoblast migration

“…Control of MMP activity occurs at several levels: transcription, activation of the precursor zymogen, and inhibition by endogenous inhibitors (tissue inhibitors of metalloproteinases). Without regulation, active MMPs can cause extensive ECM remodeling and result in a spectrum of pathological conditions, including arthritis, cancer, atherosclerosis, and fibrosis (21,24,27). However, the absence of MMP activity results in unresolved repair in bone (16), stalled development (14), and impaired wound healing (reviewed in Ref.…”