Membrane Transport in Single Cells

Abstract

The sections in this article are: Examples of Preparations of Single Cells Large Cells that Can Be Studied Individually Blood Cells Cells Prepared by Dissociating Intact Tissue Permanent Cell Lines and Expression Systems Methods for Measuring Transport Tracer Influx and Efflux: Definition of Flux Definition of Permeability Coefficient Unstirred Layers Multiple Intracellular Compartments Optical Methods for Measuring Transport Ion‐Sensitive Microelectrodes Membrane Potential Nuclear Magnetic Resonance Transport Mechanisms Rationale for Investigating Transport Mechanisms Catalytic Cycles Anion Exchanger Red Blood Cell Anion Transport Electrically Silent Obligatory Exchange Kinetic Mechanism of Anion Exchange Intrinsic Asymmetries Electrical Properties of the Exchange Pathway Sodium Pump Electrically Silent and Electrogenic Na Pump Modes Pump Stoichiometry Kinetic Mechanism of the Na Pump Electrical Properties of the Na Pump Summary of Transport Mechanisms Integrative Transport Studies in Single Cells: Cell‐Volume Regulation Pump‐Leak Paradigm Volume‐Sensitive Transport Processes Detection of Cell Volume Transduction of Volume Signal to Transport Proteins