Membrane Stabilization of Biodegradable Polymersomes

Katz, Joshua S.; Levine, Dalia H.; Davis, K.; Bates, Frank S.; Hammer, Daniel A.; Burdick, Jason A.

doi:10.1021/la803769q

Cited by 33 publications

(28 citation statements)

References 31 publications

(53 reference statements)

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“…Vesicles made from biodegradable and bioresorbable polymers have wide appeal for in vivo use as they can facilitate both sustained release of therapeutic drugs and imaging (19,20). Polymersomes made through self assembly of the diblock copolymer polyethyleneoxide (PEO)-b-polycaprolactone (PCL) hydrolytically degrade at the PCL block ester linkage (21).…”

Section: Resultsmentioning

confidence: 99%

Sensing membrane stress with near IR-emissive porphyrins

Kamat

Liao

Moses

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Probes embedded within a structure can enable prediction of material behavior or failure. Carefully assembled composites that respond intelligently to physical changes within a material could be useful as intrinsic sensors. Molecular rotors are one such tool that can respond optically to physical environmental changes. Here, we propose to use molecular rotors within a polymersome membrane to report membrane stress. Using supermolecular porphyrin-based fluorophores as rotors, we characterize changes in the optical emission of these near-infrared (NIR) emissive probes embedded within the hydrophobic core of the polymersome membrane. The configuration of entrapped fluorophore depends on the available space within the membrane; in response to increased volume, emission is blue shifted. We used this feature to study how shifts in fluorescence correlate to membrane integrity, imparted by membrane stress. We monitored changes in emission of these porphyrinbased fluorophores resulting from membrane stress produced through a range of physical and chemical perturbations, including surfactant-induced lysis, hydrolytic lysis, thermal degradation, and applied stress by micropipette aspiration. This paper comprehensively illustrates the potential for supermolecular porphyrin-based fluorophores to detect intrinsic physical changes in a wide variety of environments, and suggests how molecular rotors may be used in soft materials science and biology as sensors.fluorescent stress sensor | rheology | soft matter

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Section: Resultsmentioning

confidence: 99%

Sensing membrane stress with near IR-emissive porphyrins

Kamat

Liao

Moses

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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“…This cross-linking method is used extensively in the formation of hydrogels 37 but has been sparingly used to modify polymer vesicles. 38 By optimizing this method, we ensured minimal damage to proteins or functional groups attached to the polymer from free-radicals in solution.…”

Section: Resultsmentioning

confidence: 99%

Effects of membrane rheology on leuko-polymersome adhesion to inflammatory ligands

Robbins¹,

Lee²,

Katz³

et al. 2011

Soft Matter

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Summary A strategy for treating inflammatory disease is to create micro-particles with the adhesive properties of leukocytes. The underlying rheology of deformable adhesive microspheres would be an important factor in the adhesive performance of such particles. In this work the effect of particle deformability on the selectin-mediated rolling of polymer vesicles (polymersomes) is evaluated. The rheology of the polymersome membrane was modulated by cross-linking unsaturated side-chains within the hydrophobic core of the membrane. Increased membrane rigidity resulted in decreased rates of particle recruitment rather than decreased average rolling velocities. Reflective interference contrast microscopy of rolling vesicles confirmed that neither flaccid nor rigid vesicles sustained close contacts with the substrate during rolling adhesion. A variable-shear rate parallel-plate flow chamber was employed to evaluate individual vesicles rolling on substrates under different flow conditions. Analysis of the trajectories of single flaccid vesicles revealed several distinct populations of rolling vesicles; however, some of these populations disappear when the vesicle membranes are made rigid. This work shows that membrane mechanics affects the capture, but not the rolling dynamics, of adherent leuko-polymersomes

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“…At different time intervals, 0.1 mL aliquots of the mixture were withdrawn, diluted with 1.0 mL of Tris buffer, and then the fluorescence (F i ) was measured (excitation maximum, 490 nm; emission maximum, 520 nm). To obtain the maximal fluorescence (F T ), 0.05 mL of a freshly eluted fraction was disrupted with 3% (w/v) Triton X-100, 30,43 the final concentration of which was 0.1% (w/v), followed by diluting to 3.575 mL with Tris buffer before recording the fluorescence. The extent of release (%) over time was calculated from (F i /F T )×100.…”

Section: 폴리머 제37권 제3호 2013년mentioning

confidence: 99%

Characterization and Release Behavior of Polymersomes of PEG-Poly(fumaric-sebacic acids)-PEG Triblock Copolymer in Aqueous Solution

Pourhosseini¹,

Saboury²,

Najafi³

et al. 2013

Polymer Korea

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Polymersomes made of biodegradable triblock copolymers based on poly(fumaric acid-co-sebacoyl chloride)/ PEG (PEG-co-P(FA/SC)-co-PEG) were prepared and studied in aqueous solutions. TEM confirmed the formation of vesicles in aqueous media. Aggregation behavior of the copolymers was studied by fluorescence spectroscopy of 8-anilino-1-naphthalenesulfonic acid, and the critical aggregation concentration (c.a.c.) of the copolymer was found to be ~26.2 µM indicating desirable stability of the vesicles. Dynamic light scattering revealed that the size of the vesicles was distributed within the range of 170-270 nm. Turbidity measurements confirmed the relative short-term stability of the polymersomes. Carboxyfluorescein, a hydrophilic compound, was simply encapsulated in the vesicles during polymersome preparation. The release of encapsulant from the polymersomes at 25 and 37 o C lasted about 3 weeks, and the rate of release followed a first-order kinetics. The release is speculated to be primarily carried out through diffusion. These results confirm that these polymersomes are promising as controlled-release carriers of various drugs.

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Membrane Stabilization of Biodegradable Polymersomes

Cited by 33 publications

References 31 publications

Sensing membrane stress with near IR-emissive porphyrins

Sensing membrane stress with near IR-emissive porphyrins

Effects of membrane rheology on leuko-polymersome adhesion to inflammatory ligands

Characterization and Release Behavior of Polymersomes of PEG-Poly(fumaric-sebacic acids)-PEG Triblock Copolymer in Aqueous Solution

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