Membrane Reorganization during Secretion in Tetrahymena

Satir, Birgit H.; Schooley, Caroline; Satir, Peter

doi:10.1038/235053a0

Cited by 106 publications

(44 citation statements)

References 7 publications

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“…The recent visualization of translational mobility of antigens on the surface of virus-fused heterokaryons (23), lymphocyte capping (40), lateral diffusion of antimuscle plasma membrane antibody (18), and rosette formation during mucocyst secretion (67), all suggest that membrane fluidity (70) affords the mechanism for the aggregation of particles into gap junctions. Since the gap junction is probably the site of electrotonic coupling and intercellular transport of larger molecules (5,66), the particles must be exposed to an environment that is successively hydrophilic, then hydrophobic, and ultimately hydrophilic again.…”

Section: Gap Junction Formation Within the Plasma Membranementioning

confidence: 99%

Assembly of Gap Junctions During Amphibian Neurulation

Decker

Friend

1974

The Journal of Cell Biology

242

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Sequential thin-section, tracer (K-pyroantimonate, lanthanum, ruthenium red, and horseradish peroxidase), and freeze-fracture studies were conducted on embryos and larvae of Rana pipiens to determine the steps involved in gap junction assembly during neurulation. The zonulae occludentes, which join contiguous neuroepithelial cells, fragment into solitary domains as the neural groove deepens. These plaque-like contacts also become permeable to a variety of tracers at this juncture. Where the ridges of these domains intersect, numerous 85-A participles apparently pile up against tight junctional remnants, creating arrays recognizable as gap junctions. With neural fold closure, the remaining tight junctional elements disappear and are replaced by macular gap junctions. Well below the junctional complex, gap junctions form independent of any visible, preexisting structure. Small, variegated clusters, containing 4-30 particles located in flat, particle-free regions, characterize this area. The number of particles within these arrays increases and they subsequently blend together into a polygonally packed aggregate resembling a gap junction. The assembly process in both apical and basal regions conforms with the concept of translational movement of particles within a fluid plasma membrane.

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Section: Gap Junction Formation Within the Plasma Membranementioning

confidence: 99%

Assembly of Gap Junctions During Amphibian Neurulation

Decker

Friend

1974

The Journal of Cell Biology

242

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“…THE MUCOCYSTS (Figure I c) are cortical organelles; they are shown in the intact and extruding form. Mucocysts have been studied extensively both structurally (140,3,143,144,122,123,120,92) and chemically (1, 50); however, the biogenesis of the organelles has not been revealed in detail. Tetrahymena contains about 1000 mucocysts; the extruded mucocyst material binds the cationic dye, alcian blue (90,92,100,138), and the possible role of this material in nutrient uptake has been discussed (92,97).…”

Section: The Cell Organellesmentioning

confidence: 99%

On cell organelles in Tetrahymena. With special reference to mitochondria and peroxisomes

Nilsson

1981

Carlsberg Res. Commun.

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In the ciliate, Tetrahymena pyriformis, the population of cell organelles shows variation during the culture cycle and when the cells are subjected to various treatments. New cell constituents may appear and the fine structure of mitochondria and peroxisomes may change. Such dynamic structural changes are described and reviewed in relation to physiological and biochemical studies on Tetrahymena. The cell organelles discussed are about I tam in diameter or smaller~ reference is also made to the nuc[eotar organization which is the most sensitive indicator of the physiologica~ state of the ceils. The presumed distribution of the various cell organelles in subcellular fractions of Tetrahymena is mentioned, since dependent on the pretreatment of the cells, especially the mitochondrial and peroxisomal fractions may be contaminated to varying degrees with other cell organelles~The purpose of the review is to demonstrate that a correlation exists between the structure and function of cell organelles, especially mitochondria and peroxisomes, and that the overall fine structure of a cell reflects its physiological state.

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“…Using these techniques, Satir and co-workers (33,34) observed membrane fusion during mucocyst secretion in Tetrahymena and proposed a model for membrane reorganization during fusion which is qualitatively different from that of Palade and Bruns. Briefly, a rosette of membrane particles (thought to represent protein-containing intramembrane structures) was formed before fusion.…”

mentioning

confidence: 99%

“…The formation of bilayer membrane diaphragms during fusion can be found in micrographs included in the freeze-fracture study of membrane fusion during mucocyst secretion (33,34)? Here, a rosette of particles (diameter -60 nm) appears on the region of the plasma membrane which is fusing with the secretory vesicle.…”

mentioning

confidence: 99%

Membrane fusion during secretion. A hypothesis based on electron microscope observation of Phytophthora Palmivora zoospores during encystment.

Silva¹,

Nogueira²

1977

The Journal of Cell Biology

151

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Interpretation of freeze-fracture and thin-section results shows that fusion of the peripheral vesicle with the plasmalemma of a Phytophthora palmivora zoospore occurs at several discrete sites and results in the formation and expansion of a particle-free bilayer membrane diaphragm and in the appearance of a polymorphic network of membrane-bounded tunnels, the lumina of which are continuous with the cytoplasm. The outer half of the bilayer membrane diaphragm appears continuous with the outer half of the plasma membrane; the inner half of the bilayer membrane diaphragm with the inner half of the peripheral vesicle membrane; and the inner half of the plasmalemma with the outer half of the peripheral vesicle membrane.Interpretation of our results leads us to formulate a hypothesis for a sequence of several intermediate stages involved in membrane fusion. The initial fusion event is viewed as a local catastrophe (Thorn, R. 1972. Stabilit6 Structurelle et Morphogen~se. W. A. Benjamin Inc., Reading, Mass.) involving the sudden reorganization of apposed elements of the inner half of the plasmalemma and the outer half of the peripheral vesicle membrane. Fusion of apposed components at the rim of the perimeter of fusion results in the formation of a toroid hemi-micelle which provides continuity between the inner half of the plasmalemma and the outer half of the peripheral vesicle membrane. Simultaneously, apposed components at the site of fusion may reorganize into an inverted membrane micelle. A bilayer membrane diaphragm is then formed by apposition and flowing of components form the outer half of the plasmalemma and the inner (exoplasmic) half of the peripheral vesicle membrane. The existence of large areas of membrane contact before fusion may lead to several fusion events and the formation of a polymorphic network of membrane-bound tunnels.Membrane fusion is a frequent and important event in the life of most eukaryotic cells (17,23,29,30). Among other cellular processes, merebrane fusion mediates secretion as a necessary step to the transfer of macromolecules contained within cytoplasmic vesicles to the cell periphery or

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Membrane Reorganization during Secretion in Tetrahymena

Cited by 106 publications

References 7 publications

Assembly of Gap Junctions During Amphibian Neurulation

Assembly of Gap Junctions During Amphibian Neurulation

On cell organelles in Tetrahymena. With special reference to mitochondria and peroxisomes

Membrane fusion during secretion. A hypothesis based on electron microscope observation of Phytophthora Palmivora zoospores during encystment.

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