Membrane reinforcement in giant hybrid polymer lipid vesicles achieved by controlling the polymer architecture

Fauquignon, Martin; Ibarboure, Emmanuel; Meins, Jean-François Le

doi:10.1039/d0sm01581d

Cited by 14 publications

(46 citation statements)

References 43 publications

(39 reference statements)

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“…This phenomenon was also observed with diblock copolymers PDMS27-b-PEO17 and PDMS23-b-PEO13 when forming GHUVs. (29) This particular shape of GHUVs can provide insight into the boundary energy between the polymer and the lipid phases, also known as line tension. In Figure 2, a schematic of the hybrid polymer/lipid membrane with lateral phase separation is shown for clarification.…”

Section: Resultsmentioning

confidence: 99%

“…Finally, this could explain the differences in phase diagrams that have been established in previous studies for GHUVs obtained with a mixture of POPC and these diblock and triblock copolymers. (18,19) For example, for a same lipid composition of 30 wt.% a majority of GHUVs made from triblock copolymers PEO8-b-PDMS22-b-PEO8 show micrometer-size domains whereas only 10% of the GHUVs present a phase separation in the case of diblock PDMS23-b-PEO13. (18,19) Given the higher line tension for PEO8-b-PDMS22-b-PEO8/POPC, the system tends to form lipid microdomains more easily when increasing the lipid fraction.…”

Section: Figure 4 Example Of the Evolution Of The Suction Pressure As A Function Of The Geometrical Parameter (1/a) For The Hybrid Systemmentioning

confidence: 99%

“…(18,19) For example, for a same lipid composition of 30 wt.% a majority of GHUVs made from triblock copolymers PEO8-b-PDMS22-b-PEO8 show micrometer-size domains whereas only 10% of the GHUVs present a phase separation in the case of diblock PDMS23-b-PEO13. (18,19) Given the higher line tension for PEO8-b-PDMS22-b-PEO8/POPC, the system tends to form lipid microdomains more easily when increasing the lipid fraction. These domains are formed by the coalescence of nanodomains, in analogy to what has been observed with multiphase lipid vesicles, (39) to limit the boundary energy cost.…”

Section: Figure 4 Example Of the Evolution Of The Suction Pressure As A Function Of The Geometrical Parameter (1/a) For The Hybrid Systemmentioning

confidence: 99%

“…Giant hybrid unilamellar vesicles (GHUVs) obtained with triblock copolymers present stable budded lipid domains, as demonstrated in a previous study. (18) These budded vesicles were also obtained with diblock copolymers, (19) allowing line tension measurement by the micropipette technique. We will show and discuss the importance of block copolymer architecture and hydrophobic length mismatch at the polymer/lipid boundaries on the line tension and the consequences in terms of membrane structuration.…”

Section: Introductionmentioning

confidence: 99%

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Hybrid polymer/lipid vesicles: Influence of polymer architecture and molar mass on line tension

Fauquignon¹,

Ibarboure²,

Meins³

2022

Biophysical Journal

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Hybrid polymer/lipid vesicles are self-assembled structures that have been the subject of an increasing number of studies in recent years. They are particularly promising tools in the development of cell membrane models as they offer the possibility to fine-tune their membrane structure by adjusting the distribution of components (presence or absence of "raft like" lipid domains) which is of prime importance to control their membrane properties. Line tension in multiphase membranes is known to be a key parameter on membrane structuration but remains unexplored, either experimentally or by computer modelling for hybrid polymer /lipid vesicles. In this study we were able to measure the line tension on different budded hybrid vesicles, using micropipette aspiration technique, and show the influence of the molar mass and the architecture of block copolymers on line tension and its consequences for membrane structuration.

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Section: Resultsmentioning

confidence: 99%

Section: Figure 4 Example Of the Evolution Of The Suction Pressure As A Function Of The Geometrical Parameter (1/a) For The Hybrid Systemmentioning

confidence: 99%

Section: Figure 4 Example Of the Evolution Of The Suction Pressure As A Function Of The Geometrical Parameter (1/a) For The Hybrid Systemmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hybrid polymer/lipid vesicles: Influence of polymer architecture and molar mass on line tension

Fauquignon¹,

Ibarboure²,

Meins³

2022

Biophysical Journal

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“…Various molecular properties of the polymer blocks such as polydispersity, charge, block ratio and length, and molecular weight are essential to induce the supramolecular assemblies formed thereof. The addition of biomolecules changes the properties of the polymer membranes, the integration of phospholipids can change membrane mechanical properties [ 5 , 6 , 7 ], and membrane proteins can alter membrane permeability [ 8 ] and even provide the desired functionality via their intrinsic bioactivity [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Current Perspectives on Synthetic Compartments for Biomedical Applications

Heuberger

Korpidou

Eggenberger

et al. 2022

IJMS

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Nano- and micrometer-sized compartments composed of synthetic polymers are designed to mimic spatial and temporal divisions found in nature. Self-assembly of polymers into compartments such as polymersomes, giant unilamellar vesicles (GUVs), layer-by-layer (LbL) capsules, capsosomes, or polyion complex vesicles (PICsomes) allows for the separation of defined environments from the exterior. These compartments can be further engineered through the incorporation of (bio)molecules within the lumen or into the membrane, while the membrane can be decorated with functional moieties to produce catalytic compartments with defined structures and functions. Nanometer-sized compartments are used for imaging, theranostic, and therapeutic applications as a more mechanically stable alternative to liposomes, and through the encapsulation of catalytic molecules, i.e., enzymes, catalytic compartments can localize and act in vivo. On the micrometer scale, such biohybrid systems are used to encapsulate model proteins and form multicompartmentalized structures through the combination of multiple compartments, reaching closer to the creation of artificial organelles and cells. Significant progress in therapeutic applications and modeling strategies has been achieved through both the creation of polymers with tailored properties and functionalizations and novel techniques for their assembly.

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Controlling plasmonic suprastructures through self-assembly of gold nanoparticles with hybrid copolymer-lipid vesicles

Cardellini,

Balestri,

Comparini

et al. 2024

Journal of Colloid and Interface Science

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Membrane reinforcement in giant hybrid polymer lipid vesicles achieved by controlling the polymer architecture

Abstract: Physical properties of membranes of hybrid polymer lipid vesicles are so far relatively unknown. Since their discovery a decade ago, many studies have aimed to show their high potential in...

Cited by 14 publications

References 43 publications

Hybrid polymer/lipid vesicles: Influence of polymer architecture and molar mass on line tension

Hybrid polymer/lipid vesicles: Influence of polymer architecture and molar mass on line tension

Current Perspectives on Synthetic Compartments for Biomedical Applications

Controlling plasmonic suprastructures through self-assembly of gold nanoparticles with hybrid copolymer-lipid vesicles

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