Membrane proteins specified by herpes simplex viruses. III. Role of glycoprotein VP7(B2) in virion infectivity

Abstract

Experiments done with a temperature-sensitive mutant of herpes simplex virus type 1 (HSV-1) have revealed that one of the virion glycoproteins, designated VP7(B2), is apparently not required for the production of enveloped virus particles, whereas it does play a critical role in virion infectivity. The mutant, designated HSV-l[HFEM]tsB5, fails to accumulate VP7(B2) at nonpermissive temperature and produces virions that lack detectable quantities of this glycoprotein and that have very low specific infectivity. The poor infectivity of the virions is most readily explained by failure of penetration into the host cell rather than by failure of adsorption to cells because it was shown that the VP7(B2)-deficient virions can bind to cells and that polyethylene glycol, an agent known to promote membrane fusion, can significantly enhance infectivity of the adsorbed virions.

Inhibitors of protein glycosylation and glycoprotein processing in viral systems

“…These similarities between HSV and EBV suggest similarities in the proteins mediating fusion for EBV and HSV. As pointed out above, the HSV-specified glycoprotein B is involved in viral penetration (Sarmiento et al, 1979). The predicted sequence of the EBVspecified glycoprotein gp85 is in part similar to the sequence of the HSV glycoprotein B (Pellet et al, 1985;Baer et al, 1984).…”

“…In studies the HSV-1 induced syncytia formation, it has been found that both gB and gD have fusogenic properties (Noble et al, 1983;Manservigi et al, 1977;Little et al, 1981). At least gB seems to be essential for the penetration of HSV-I as temperature-sensitive, gB-negative mutants when produced at non-permissive temperature have the ability to adsorb to permissive cells, but cannot penetrate (Manservigi et al, 1977;Little et al, 1981;Sarmiento et al, 1979). The role of gD in penetration is unknown.…”

Virus Entry into Animal Cells

“…The gB protein is essential for entry and appears to be involved in fusion but not in binding (Ali et al, 1987). A role in fusion has also been suggested for the gD protein (Ali et al, 1987;Campadelli-Fiume et al, 1988;Fuller and Spear, 1987;Sarnieto et al, 1979). Morphological studies indicate that virions are internalized, and that this may occur in coated vesicles.…”

Effect of tunicamycin and monensin on biosynthesis, transport, and maturation of bovine herpesvirus type-1 glycoproteins

“…As such, they play an important role during viral infection in a number of ways. Not only do they recognize receptor sites for the attachment and penetration of a virus into the cell (Manservigi et u& 1977;Sarmiento et a& 1979), they are also the antigens that determine 'Author to whom reprint requests should be addressed. the host's immune response to the virus.…”