“…A particular highlight is the prokaryotic holo-translocon complex (HTL), a seven subunit transmembrane multiprotein assembly consisting of the heterotrimeric core translocon, SecYEG, and its accessory proteins SecD, SecF, YidC and YajC. HTL is a long elusive complex that was, for the first time, successfully produced recombinantly by ACEMBL [63,64]. HTL catalyzes the transport of protein substrates through and into membranes, making use of the proton motive force (PMF) [63].…”