Membrane protein biosensing with plasmonic nanopore arrays and pore-spanning lipid membranes

Im, Hyungsoon; Wittenberg, Nathan J.; Lesuffleur, Antoine; Lindquist, Nathan C.; Oh, Sang‐Hyun

doi:10.1039/c0sc00365d

Cited by 118 publications

(141 citation statements)

References 74 publications

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Section: Discussionmentioning

confidence: 99%

“…This mechanism is based on the following steps: yeast DNA glucose sensor cells become in contact with glucose molecule, ion channel in the yeast cell membrane becomes activated, then second messengers system for intracellular glucose transport (i.e. GLUT and Kinases) in the cytoplasm induce the metabolic cascade toward activation of the DNA sensor in the nucleus, following transcription of DNA sensor in the nucleus towards production of the fluorescence reporter protein, and finally the excitation of bio-photons for emission of fluorescence according to glucose concentration occurs [40][41][42].2D-DIGE and LC-MS/MS analysis have demonstrated that our genetic glucose sensor device is able to induce a differential response in a yeast DNA sensor, compared to a non-transformed yeast control, even when both yeast systems are grown under similar conditions and similar glucose concentrations. Proteins involved in, or related to, glucose metabolism were expressed at high levels.…”

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confidence: 99%

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Construct of DNA glucose sensor yeast plasmid for early detection of diabetes

Cuero¹,

Navia²,

Agudelo³

et al. 2017

Integr Obesity Diabetes

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This investigation was aimed at assembling different genetic building blocks to produce a focused DNA sensor to detect proteins related to glucose in blood, in order to diagnose early diabetes using synthetic biology and conventional molecular biology. A glucose DNA sensor was constructed in Saccharomyces cerevisiae using genomic and synthesized sequences and were tested in both, In vivo using human blood plasma sampled, and In vitro using cultural media. The results were based on fluorescence intensity of the DNA sensor and compared with clinical methods. The DNA sensor was highly sensitive when mixed with plasma samples from different patients (i.e., diabetic, pre-diabetic, and normal) showing high fluorescence, and was able to detect a wide concentration range of glucose equivalent to clinical glycaemia values. Expression of proteins pertaining to glucose metabolism production was determined by 2D-DIGE gel electrophoresis-maldi analysis. The glucose sensor produced results less than a minute after being mixed with a drop of a human blood sample. Our results highlight the advantages of using constructed DNA sensors to detect glucose in blood for early diagnosis of diabetes.The yeast DNA glucose sensor was assembled successfully with standardized genetic parts and was able to detect low levels equivalent to clinical glycaemia (<140 mg/ dL), as well as a higher equivalent level of glycaemia (>200 mg/dL). Thus, our sensor can be used for early diagnosis of diabetes or pre-diabetic conditions, thereby allowing for earlier clinical intervention. The direct correlation between levels of glucose and the intensity of fluorescence of the DNA sensor shows the advantage of using this technology in order to identify specific types of diabetic patients. Sometimes, it is difficult to establish accurate correlations with different methods.human blood [7]. These types of sensors are versatile analytical tools that offer advantages over classical analytical methods due to their inherent specificity, selectivity, and simplicity [5]. Most biological and cellular sensors use membrane and cellular proteins that recognize and take up biomarkers from a medium [6]. However, DNA has also been used in molecular biosensors [8,9].Biomarkers for detecting and diagnosing diseases include physical symptoms, mutated DNA and RNA, secreted proteins, processes such as cell death or proliferation, and serum concentrations of small molecules such as cholesterol or, in the case of diabetes, glucose [6].Currently, the most common methods to diagnose diabetes use enzymatic reactions to determine glucose levels [10,11]. These methods measure glucose in whole blood because erythrocytes contain high concentrations of glucose [12]. The methods include the use of glucose oxidase, hexokinase, and glucose dehydrogenase [13][14][15]. The products of these reactions with blood sugar can be determined using colorimetric and spectrophotometric assays or by measuring the electric current produced in the enzyme reactions; the latter is the approach used ...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Construct of DNA glucose sensor yeast plasmid for early detection of diabetes

Cuero¹,

Navia²,

Agudelo³

et al. 2017

Integr Obesity Diabetes

View full text Add to dashboard Cite

show abstract

“…Moreover, SangHyun Oh developed a plasmonic nanopore using a gold nanohole array to detect the incorporation of a transmembrane protein, α-haemolysin (α-HL), as shown in Fig. 5.17 [51]. α-HL is a water-soluble peptide monomer (33.2 kD) secreted from the pathogenic bacteria Staphylococcus aureus that binds to the plasma membranes of numerous mammalian cell types.…”

Section: Plasmonic Nanoporesmentioning

confidence: 99%

Interparticle Coupling-Enhanced Detection

Long

Jing

2014

SpringerBriefs in Molecular Science

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When the distances between two or more plasmonic nanoparticles are very small, the plasmon resonance scattering spectra are greatly enhanced and distinct colour changes occur due to the coupling of the particles. Similar to fluorescence resonance energy transfer, plasmonic coupling is also distance dependent. Thus, researchers have fabricated colorimetric sensors by modulating the distance between nanoparticles, which have been used in a wide variety of applications, including DNA hybridisation, heavy-metal-ion detection, and protein binding. In this chapter, we primarily focus on the coupling of single particles, which enables the single-molecule detection through enhanced sensitivity. Keywords Fundamentals of Plasmonic CouplingThe coupling of plasmonics enhances the resonance intensity significantly and enables variable sensitive biosensors [1][2][3][4][5][6][7][8][9][10][11]. The factors that affect the coupling of nanoparticles include their particle size, coupling number, distance, direction, and shape [12][13][14][15][16][17]. Lee calculated the influence of particle size, number, composition, and distance using the generalised multiparticle Mie formalism [18]. The satellites in this work were made of gold with a relative permeability μ = 1. Cores composed of either gold or glass were considered. The surrounding medium was assumed to be free space with a permittivity ε = μ = 1. The permittivity of the glass core was 2.25. As shown in Fig. 5.1a, as the satellite number increased, the scattering peak wavelength exhibited a nearly linear redshift. For r core = 50 nm, r sat = 10 nm, and d sat = 2 nm, the redshift was approximately 1 nm per satellite. Although these calculations may not accurately reflect absolute quantities, they reveal the trends in the peak wavelength as the number of satellites increases.

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“…Fluorescence is the most likely optical technique to be used in conjunction with a nanopore, but one could imagine that a suitably structured gold film, separated by a well-defined nanopore gap containing an analyte, might also be used for surface-enhanced Raman spectroscopy. 20,27,46,61,90,[267][268][269][305][306][307][308][309][310][311][312][313][314][315] Extensions to Nanopore Arrays. We conclude our discussion of the merging of physical-and optical-based nanopore sensing and manipulation approaches, with the consideration of nanopore arrays.…”

Section: Nanopore As Nanofluidic Cuvette and Optofluidic Devicementioning

confidence: 99%

“…[21][22][23][24][25][26][27][28][29] Extension of this single pore design to many pores allows the same thin-film platform to function as a high-throughput filter, with implications also for nanoplasmonics. 60,61 There are excellent reviews focusing on these two particular implementations alone, [21][22][23][24][25][26][27][28][29]38,40 and a recent book chapter reviewing both in a unifying context of nanofluidics. 20 We hope the present review will encourage an appreciation of the breadth of application of even an ostensibly prosaic free-standing thin-film structure across a host of spectroscopy, microscopy, and sensing techniques.…”

Section: Introductionmentioning

confidence: 99%

Through a Window, Brightly: A Review of Selected Nanofabricated Thin-Film Platforms for Spectroscopy, Imaging, and Detection

Dwyer

Harb

2017

Appl Spectrosc

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We present a review of the use of selected nanofabricated thin films to deliver a host of capabilities and insights spanning bioanalytical and biophysical chemistry, materials science, and fundamental molecular-level research. We discuss approaches where thin films have been vital, enabling experimental studies using a variety of optical spectroscopies across the visible and infrared spectral range, electron microscopies, and related techniques such as electron energy loss spectroscopy, X-ray photoelectron spectroscopy, and single molecule sensing. We anchor this broad discussion by highlighting two particularly exciting exemplars: a thin-walled nanofluidic sample cell concept that has advanced the discovery horizons of ultrafast spectroscopy and of electron microscopy investigations of in-liquid samples; and a unique class of thin-film-based nanofluidic devices, designed around a nanopore, with expansive prospects for single molecule sensing. Free-standing, low-stress silicon nitride membranes are a canonical structural element for these applications, and we elucidate the fabrication and resulting features-including mechanical stability, optical properties, X-ray and electron scattering properties, and chemical natureof this material in this format. We also outline design and performance principles and include a discussion of underlying material preparations and properties suitable for understanding the use of alternative thin-film materials such as graphene.

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Membrane protein biosensing with plasmonic nanopore arrays and pore-spanning lipid membranes

Cited by 118 publications

References 74 publications

Construct of DNA glucose sensor yeast plasmid for early detection of diabetes

Construct of DNA glucose sensor yeast plasmid for early detection of diabetes

Interparticle Coupling-Enhanced Detection

Through a Window, Brightly: A Review of Selected Nanofabricated Thin-Film Platforms for Spectroscopy, Imaging, and Detection

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